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- W2169063908 endingPage "3449" @default.
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- W2169063908 abstract "SUMMARY Cauliflower mosaic virus (CaMV) is a DNA plant virus that replicates its genome through an RNA intermediate. The cytoplasmic step of CaMV DNA replication was studied using a fraction consisting of purified viroplasms, which are virus-specific inclusion bodies accumulating in the infected plant cells. The isolated viroplasms retain a DNA polymerase activity able to synthesize CaMV DNA from endogenous templates. A further characterization of the viral DNA sequences produced in the isolated inclusion bodies indicates that newly synthesized DNA, mostly of polarity opposite to that of viral RNA, is single-stranded and partly associated with RNA by base-pairing. In addition to an RNA-dependent DNA polymerase activity, RNA molecules, which presumably originate from the viral RNA template used for reverse transcription, are found to accumulate in the purified inclusion bodies. Furthermore, a small DNA molecule strongly labelled in the purified fraction has been characterized and corresponds to the CaMV reverse transcription intermediate sa-DNA. These results provide further evidence that the reverse transcription of CaMV RNA occurs in the viroplasms. Additional data are presented which suggest that CaMV replication could occur in virion-related particles." @default.
- W2169063908 created "2016-06-24" @default.
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- W2169063908 date "1989-12-01" @default.
- W2169063908 modified "2023-10-18" @default.
- W2169063908 title "Further Evidence that Viroplasms Are the Site of Cauliflower Mosaic Virus Genome Replication by Reverse Transcription during Viral Infection" @default.
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- W2169063908 doi "https://doi.org/10.1099/0022-1317-70-12-3439" @default.
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