FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169187316> ?p ?o ?g. }

• W2169187316 endingPage "5359" @default.
• W2169187316 startingPage "5351" @default.
• W2169187316 abstract "The budded virus (BV) of the Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) infects insect cells and transduces mammalian cells mainly through the endocytosis pathway. However, this study revealed that the treatment of the virus bound to Sf9 cells at low pH could efficiently rescue the infectivity of AcMNPV in the presence of endocytosis pathway inhibitors. A colocalization assay of the major capsid protein VP39 with the early endosome marker EEA1 showed that at low pH, AcMNPV entered Sf9 cells via an endosome-independent pathway. Using a fluorescent probe (R18), we showed that at low pH, the viral nucleocapsid entered Sf9 cells via direct fusion at the cell surface. By using the myosin-specific inhibitor 2,3-butanedione monoxime (BDM) and the microtubule inhibitor nocodazole, the low pH-triggered direct fusion was demonstrated to be dependent on myosin-like proteins and independent of microtubules. The reverse transcription-PCR of the IE1 gene as a marker for viral entry showed that the kinetics of AcMNPV in cells triggered by low pH was similar to that of the normal entry via endocytosis. The low pH-mediated infection assay and VP39 and EEA1 colocalization assay also demonstrated that AcMNPV could efficiently transduce mammalian cells via direct membrane fusion at the cell surface. More importantly, we found that a low-pH trigger could significantly improve the transduction efficiency of AcMNPV in mammalian cells, leading to the potential application of this method when using baculovirus as a vector for heterologous gene expression and for gene therapy." @default.
• W2169187316 created "2016-06-24" @default.
• W2169187316 creator A5010514395 @default.
• W2169187316 creator A5057120703 @default.
• W2169187316 creator A5074683992 @default.
• W2169187316 creator A5077451991 @default.
• W2169187316 creator A5080868447 @default.
• W2169187316 date "2010-05-15" @default.
• W2169187316 modified "2023-09-27" @default.
• W2169187316 title "<i>Autographa californica</i> Multicapsid Nucleopolyhedrovirus Efficiently Infects Sf9 Cells and Transduces Mammalian Cells via Direct Fusion with the Plasma Membrane at Low pH" @default.
• W2169187316 cites W108460990 @default.
• W2169187316 cites W1503858009 @default.
• W2169187316 cites W1574015045 @default.
• W2169187316 cites W1903799742 @default.
• W2169187316 cites W1964315952 @default.
• W2169187316 cites W1964637148 @default.
• W2169187316 cites W1965229979 @default.
• W2169187316 cites W1982781424 @default.
• W2169187316 cites W1986690418 @default.
• W2169187316 cites W1990917234 @default.
• W2169187316 cites W1993905851 @default.
• W2169187316 cites W2006286994 @default.
• W2169187316 cites W2014128640 @default.
• W2169187316 cites W2015201004 @default.
• W2169187316 cites W2015636028 @default.
• W2169187316 cites W2021345893 @default.
• W2169187316 cites W2022339063 @default.
• W2169187316 cites W2029033007 @default.
• W2169187316 cites W2033955212 @default.
• W2169187316 cites W2036378627 @default.
• W2169187316 cites W2036864390 @default.
• W2169187316 cites W2037390556 @default.
• W2169187316 cites W2041625983 @default.
• W2169187316 cites W2046147812 @default.
• W2169187316 cites W2050878437 @default.
• W2169187316 cites W2057537794 @default.
• W2169187316 cites W2064805716 @default.
• W2169187316 cites W2064977208 @default.
• W2169187316 cites W2075470662 @default.
• W2169187316 cites W2076003033 @default.
• W2169187316 cites W2079357909 @default.
• W2169187316 cites W2080374377 @default.
• W2169187316 cites W2080663615 @default.
• W2169187316 cites W2086627002 @default.
• W2169187316 cites W2089974427 @default.
• W2169187316 cites W2093578697 @default.
• W2169187316 cites W2103202783 @default.
• W2169187316 cites W2105395703 @default.
• W2169187316 cites W2116519871 @default.
• W2169187316 cites W2118155405 @default.
• W2169187316 cites W2125545448 @default.
• W2169187316 cites W2139196607 @default.
• W2169187316 cites W2139556812 @default.
• W2169187316 cites W2139932658 @default.
• W2169187316 cites W2146374988 @default.
• W2169187316 cites W2150770791 @default.
• W2169187316 cites W2158898242 @default.
• W2169187316 cites W2166673821 @default.
• W2169187316 cites W2184846754 @default.
• W2169187316 cites W2321130193 @default.
• W2169187316 cites W2346421521 @default.
• W2169187316 doi "https://doi.org/10.1128/jvi.02517-09" @default.
• W2169187316 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2863812" @default.
• W2169187316 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20219938" @default.
• W2169187316 hasPublicationYear "2010" @default.
• W2169187316 type Work @default.
• W2169187316 sameAs 2169187316 @default.
• W2169187316 citedByCount "49" @default.
• W2169187316 countsByYear W21691873162012 @default.
• W2169187316 countsByYear W21691873162013 @default.
• W2169187316 countsByYear W21691873162014 @default.
• W2169187316 countsByYear W21691873162015 @default.
• W2169187316 countsByYear W21691873162016 @default.
• W2169187316 countsByYear W21691873162017 @default.
• W2169187316 countsByYear W21691873162018 @default.
• W2169187316 countsByYear W21691873162019 @default.
• W2169187316 countsByYear W21691873162020 @default.
• W2169187316 countsByYear W21691873162021 @default.
• W2169187316 countsByYear W21691873162022 @default.
• W2169187316 countsByYear W21691873162023 @default.
• W2169187316 crossrefType "journal-article" @default.
• W2169187316 hasAuthorship W2169187316A5010514395 @default.
• W2169187316 hasAuthorship W2169187316A5057120703 @default.
• W2169187316 hasAuthorship W2169187316A5074683992 @default.
• W2169187316 hasAuthorship W2169187316A5077451991 @default.
• W2169187316 hasAuthorship W2169187316A5080868447 @default.
• W2169187316 hasBestOaLocation W21691873161 @default.
• W2169187316 hasConcept C102747710 @default.
• W2169187316 hasConcept C103878061 @default.
• W2169187316 hasConcept C104317684 @default.
• W2169187316 hasConcept C123894998 @default.
• W2169187316 hasConcept C140704245 @default.
• W2169187316 hasConcept C142613039 @default.
• W2169187316 hasConcept C142669718 @default.
• W2169187316 hasConcept C1491633281 @default.
• W2169187316 hasConcept C15152581 @default.
• W2169187316 hasConcept C153911025 @default.
• W2169187316 hasConcept C159047783 @default.

• next