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• W2169192338 endingPage "316" @default.
• W2169192338 startingPage "308" @default.
• W2169192338 abstract "Deoxyribozymes, or DNAzymes, are DNA molecules with enzymatic activity. DNAzymes with ribonuclease activity have various potential applications in biomedical and bioanalytical fields; however, most constructs have limited turnover despite optimization of reaction conditions and DNAzyme structures. A cationic comb-type copolymer accelerates DNA hybridization and strand exchange rates, and we hypothesized that the copolymer would enhance deoxyribozyme activity by promoting turnover. The copolymer did not change DNAzyme activity under single-turnover conditions, suggesting that the copolymer affects neither the folding structure of DNAzyme nor the association of a divalent cation, a catalytic cofactor, to DNAzyme. The copolymer enhanced activity of the evaluated DNAzyme over a wide temperature range under multiple-turnover conditions. The copolymer increased the DNAzyme kcat/KM by fifty-fold at 50 °C, the optimal temperature for the DNAzyme in the absence of the copolymer. The acceleration effect was most significant when the reaction temperature was slightly higher than the melting temperature of the enzyme/substrate complex; acceleration of two orders of magnitude was observed. We concluded that the copolymer accelerated the turnover step without influencing the chemical cleavage step. In contrast to the copolymer, a cationic surfactant, CTAB, strongly inhibited the DNAzyme activity under either single- or multiple-turnover conditions." @default.
• W2169192338 created "2016-06-24" @default.
• W2169192338 creator A5006462131 @default.
• W2169192338 creator A5016713165 @default.
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• W2169192338 date "2015-01-01" @default.
• W2169192338 modified "2023-10-10" @default.
• W2169192338 title "Enhancement of deoxyribozyme activity by cationic copolymers" @default.
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• W2169192338 doi "https://doi.org/10.1039/c4bm00256c" @default.
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• W2169192338 hasPublicationYear "2015" @default.
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