Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169242581> ?p ?o ?g. }
- W2169242581 endingPage "e47414" @default.
- W2169242581 startingPage "e47414" @default.
- W2169242581 abstract "Subtotal cystectomy (STC; surgical removal of ∼75% of the rat urinary bladder) elicits a robust proliferative response resulting in complete structural and functional bladder regeneration within 8-weeks. The goal of these studies was to characterize the early cellular response that mediates this regenerative phenomenon, which is unique among mammalian organ systems. STC was performed on eighteen 12-week-old female Fischer F344 rats. At 1, 3, 5 and 7-days post-STC, the bladder was harvested 2-hours after intraperitoneal injection of bromodeoxyuridine (BrdU). Fluorescent BrdU labeling was quantified in cells within the urothelium, lamina propria (LP), muscularis propria (MP) and serosa. Cell location was confirmed with fluorescently co-labeled cytokeratin, vimentin or smooth muscle actin (SMA), to identify urothelial, interstitial and smooth muscle cells, respectively. Expression of sonic hedgehog (Shh), Gli-1 and bone morphogenic factor-4 (BMP-4) were evaluated with immunochemistry. Three non-operated rats injected with BrdU served as controls. Less than 1% of cells in the bladder wall were labeled with BrdU in control bladders, but this percentage significantly increased by 5-8-fold at all time points post-STC. The spatiotemporal characteristics of the proliferative response were defined by a significantly higher percentage of BrdU-labeled cells within the urothelium at 1-day than in the MP and LP. A time-dependent shift at 3 and 5-days post-STC revealed significantly fewer BrdU-labeled cells in the MP than LP or urothelium. By 7-days the percentage of BrdU-labeled cells was similar among urothelium, LP and MP. STC also caused an increase in immunostaining for Shh, Gli-1 and BMP-4. In summary, the early stages of functional bladder regeneration are characterized by time-dependent changes in the location of the proliferating cell population, and expression of several evolutionarily conserved developmental signaling proteins. This report extends previous observations and further establishes the rodent bladder as an excellent model for studying novel aspects of mammalian organ regeneration." @default.
- W2169242581 created "2016-06-24" @default.
- W2169242581 creator A5014135786 @default.
- W2169242581 creator A5019373727 @default.
- W2169242581 creator A5029251378 @default.
- W2169242581 creator A5047859811 @default.
- W2169242581 creator A5090669869 @default.
- W2169242581 date "2012-10-12" @default.
- W2169242581 modified "2023-10-18" @default.
- W2169242581 title "Characterization of the Early Proliferative Response of the Rodent Bladder to Subtotal Cystectomy: A Unique Model of Mammalian Organ Regeneration" @default.
- W2169242581 cites W1482082078 @default.
- W2169242581 cites W1548038310 @default.
- W2169242581 cites W1562053774 @default.
- W2169242581 cites W1563645938 @default.
- W2169242581 cites W1631250427 @default.
- W2169242581 cites W1976470802 @default.
- W2169242581 cites W1983321673 @default.
- W2169242581 cites W1985516216 @default.
- W2169242581 cites W1994437017 @default.
- W2169242581 cites W1995002140 @default.
- W2169242581 cites W1997956750 @default.
- W2169242581 cites W1998358446 @default.
- W2169242581 cites W1998420703 @default.
- W2169242581 cites W1998801099 @default.
- W2169242581 cites W2000435035 @default.
- W2169242581 cites W2000476463 @default.
- W2169242581 cites W2005579212 @default.
- W2169242581 cites W2015274943 @default.
- W2169242581 cites W2017107177 @default.
- W2169242581 cites W2029719395 @default.
- W2169242581 cites W2036510473 @default.
- W2169242581 cites W2038430559 @default.
- W2169242581 cites W2042435168 @default.
- W2169242581 cites W2052578215 @default.
- W2169242581 cites W2059100129 @default.
- W2169242581 cites W2059291562 @default.
- W2169242581 cites W2069235384 @default.
- W2169242581 cites W2070147440 @default.
- W2169242581 cites W2074445743 @default.
- W2169242581 cites W2074967040 @default.
- W2169242581 cites W2076560206 @default.
- W2169242581 cites W2098102805 @default.
- W2169242581 cites W2101938395 @default.
- W2169242581 cites W2111513766 @default.
- W2169242581 cites W2117884018 @default.
- W2169242581 cites W2128065481 @default.
- W2169242581 cites W2130848408 @default.
- W2169242581 cites W2141842727 @default.
- W2169242581 cites W2147426059 @default.
- W2169242581 cites W2152056221 @default.
- W2169242581 cites W2161736629 @default.
- W2169242581 cites W2167430913 @default.
- W2169242581 cites W2240049172 @default.
- W2169242581 cites W2247149472 @default.
- W2169242581 cites W2285466600 @default.
- W2169242581 cites W2412440009 @default.
- W2169242581 cites W2412946031 @default.
- W2169242581 cites W2412967351 @default.
- W2169242581 cites W28865656 @default.
- W2169242581 cites W2124224924 @default.
- W2169242581 cites W2466668933 @default.
- W2169242581 doi "https://doi.org/10.1371/journal.pone.0047414" @default.
- W2169242581 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3470577" @default.
- W2169242581 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23077610" @default.
- W2169242581 hasPublicationYear "2012" @default.
- W2169242581 type Work @default.
- W2169242581 sameAs 2169242581 @default.
- W2169242581 citedByCount "22" @default.
- W2169242581 countsByYear W21692425812013 @default.
- W2169242581 countsByYear W21692425812014 @default.
- W2169242581 countsByYear W21692425812015 @default.
- W2169242581 countsByYear W21692425812017 @default.
- W2169242581 countsByYear W21692425812018 @default.
- W2169242581 countsByYear W21692425812019 @default.
- W2169242581 countsByYear W21692425812020 @default.
- W2169242581 countsByYear W21692425812021 @default.
- W2169242581 countsByYear W21692425812022 @default.
- W2169242581 countsByYear W21692425812023 @default.
- W2169242581 crossrefType "journal-article" @default.
- W2169242581 hasAuthorship W2169242581A5014135786 @default.
- W2169242581 hasAuthorship W2169242581A5019373727 @default.
- W2169242581 hasAuthorship W2169242581A5029251378 @default.
- W2169242581 hasAuthorship W2169242581A5047859811 @default.
- W2169242581 hasAuthorship W2169242581A5090669869 @default.
- W2169242581 hasBestOaLocation W21692425811 @default.
- W2169242581 hasConcept C105702510 @default.
- W2169242581 hasConcept C121608353 @default.
- W2169242581 hasConcept C126894567 @default.
- W2169242581 hasConcept C142724271 @default.
- W2169242581 hasConcept C16685009 @default.
- W2169242581 hasConcept C171056886 @default.
- W2169242581 hasConcept C203014093 @default.
- W2169242581 hasConcept C204232928 @default.
- W2169242581 hasConcept C2775910329 @default.
- W2169242581 hasConcept C2777310793 @default.
- W2169242581 hasConcept C2777540882 @default.
- W2169242581 hasConcept C2778260397 @default.
- W2169242581 hasConcept C2779762690 @default.