FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169283404> ?p ?o ?g. }

• W2169283404 endingPage "1045" @default.
• W2169283404 startingPage "1038" @default.
• W2169283404 abstract "<h3>Abstract</h3> Lipidoid nanoparticles (LNPs) are the delivery platform in Onpattro, the first FDA-approved siRNA drug. LNPs are also the carriers in the Pfizer-BioNTech and Moderna COVID-19 mRNA vaccines. While these applications have demonstrated that LNPs effectively deliver nucleic acids to hepatic and muscle cells, it is unclear if LNPs could be used for delivery of siRNA to neural cells, which are notoriously challenging delivery targets. Therefore, the purpose of this study was to determine if LNPs could efficiently deliver siRNA to neurons. Because of their potential utility in either applications in the central nervous system and the peripheral nervous system, we used both cortical neurons and sensory neurons. We prepared siRNA-LNPs using C12-200, a benchmark ionizable cationic lipidoid along with helper lipids. We demonstrated using dynamic light scattering that the inclusion of both siRNA and PEG-lipid provided a stabilizing effect to the LNP particle diameters and polydispersity indices by minimizing aggregation. We found that siRNA-LNPs were safely tolerated by primary dorsal root ganglion neurons. Flow cytometry analysis revealed that Cy5 siRNA delivered via LNPs into rat primary cortical neurons showed uptake levels similar to Lipofectamine RNAiMAX—the gold standard commercial transfection agent. However, LNPs demonstrated a superior safety profile whereas the Lipofectamine-mediated uptake was concomitant with significant toxicity. Fluorescence microscopy demonstrated a time-dependent increase in the uptake of LNP-delivered Cy5 siRNA in a human cortical neuron cell line. Overall, our results suggest that LNPs are a viable platform that can be optimized for delivery of therapeutic siRNAs to neural cells." @default.
• W2169283404 created "2016-06-24" @default.
• W2169283404 creator A5008445936 @default.
• W2169283404 creator A5009721344 @default.
• W2169283404 creator A5017256940 @default.
• W2169283404 creator A5029712397 @default.
• W2169283404 creator A5088167656 @default.
• W2169283404 date "2004-04-01" @default.
• W2169283404 modified "2023-10-12" @default.
• W2169283404 title "Septicemia in the United States Dialysis Population, 1991 to 1999" @default.
• W2169283404 cites W182931159 @default.
• W2169283404 cites W1957241200 @default.
• W2169283404 cites W1964580483 @default.
• W2169283404 cites W1977905350 @default.
• W2169283404 cites W1984450372 @default.
• W2169283404 cites W2018466091 @default.
• W2169283404 cites W2027988203 @default.
• W2169283404 cites W2030328539 @default.
• W2169283404 cites W2049514151 @default.
• W2169283404 cites W2049927822 @default.
• W2169283404 cites W2058610272 @default.
• W2169283404 cites W2067951404 @default.
• W2169283404 cites W2068949553 @default.
• W2169283404 cites W2082236694 @default.
• W2169283404 cites W2101396131 @default.
• W2169283404 cites W2107117652 @default.
• W2169283404 cites W2129162902 @default.
• W2169283404 cites W2171844927 @default.
• W2169283404 cites W2316376271 @default.
• W2169283404 cites W2324841714 @default.
• W2169283404 cites W2487377689 @default.
• W2169283404 cites W61657103 @default.
• W2169283404 doi "https://doi.org/10.1097/01.asn.0000119144.95922.c4" @default.
• W2169283404 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15034107" @default.
• W2169283404 hasPublicationYear "2004" @default.
• W2169283404 type Work @default.
• W2169283404 sameAs 2169283404 @default.
• W2169283404 citedByCount "147" @default.
• W2169283404 countsByYear W21692834042012 @default.
• W2169283404 countsByYear W21692834042013 @default.
• W2169283404 countsByYear W21692834042014 @default.
• W2169283404 countsByYear W21692834042015 @default.
• W2169283404 countsByYear W21692834042016 @default.
• W2169283404 countsByYear W21692834042017 @default.
• W2169283404 countsByYear W21692834042018 @default.
• W2169283404 countsByYear W21692834042019 @default.
• W2169283404 countsByYear W21692834042020 @default.
• W2169283404 countsByYear W21692834042021 @default.
• W2169283404 countsByYear W21692834042022 @default.
• W2169283404 countsByYear W21692834042023 @default.
• W2169283404 crossrefType "journal-article" @default.
• W2169283404 hasAuthorship W2169283404A5008445936 @default.
• W2169283404 hasAuthorship W2169283404A5009721344 @default.
• W2169283404 hasAuthorship W2169283404A5017256940 @default.
• W2169283404 hasAuthorship W2169283404A5029712397 @default.
• W2169283404 hasAuthorship W2169283404A5088167656 @default.
• W2169283404 hasBestOaLocation W21692834041 @default.
• W2169283404 hasConcept C104317684 @default.
• W2169283404 hasConcept C12554922 @default.
• W2169283404 hasConcept C185592680 @default.
• W2169283404 hasConcept C2908647359 @default.
• W2169283404 hasConcept C40767141 @default.
• W2169283404 hasConcept C49923114 @default.
• W2169283404 hasConcept C54009773 @default.
• W2169283404 hasConcept C55493867 @default.
• W2169283404 hasConcept C71924100 @default.
• W2169283404 hasConcept C86803240 @default.
• W2169283404 hasConcept C92087593 @default.
• W2169283404 hasConcept C95444343 @default.
• W2169283404 hasConcept C99454951 @default.
• W2169283404 hasConceptScore W2169283404C104317684 @default.
• W2169283404 hasConceptScore W2169283404C12554922 @default.
• W2169283404 hasConceptScore W2169283404C185592680 @default.
• W2169283404 hasConceptScore W2169283404C2908647359 @default.
• W2169283404 hasConceptScore W2169283404C40767141 @default.
• W2169283404 hasConceptScore W2169283404C49923114 @default.
• W2169283404 hasConceptScore W2169283404C54009773 @default.
• W2169283404 hasConceptScore W2169283404C55493867 @default.
• W2169283404 hasConceptScore W2169283404C71924100 @default.
• W2169283404 hasConceptScore W2169283404C86803240 @default.
• W2169283404 hasConceptScore W2169283404C92087593 @default.
• W2169283404 hasConceptScore W2169283404C95444343 @default.
• W2169283404 hasConceptScore W2169283404C99454951 @default.
• W2169283404 hasIssue "4" @default.
• W2169283404 hasLocation W21692834041 @default.
• W2169283404 hasLocation W21692834042 @default.
• W2169283404 hasOpenAccess W2169283404 @default.
• W2169283404 hasPrimaryLocation W21692834041 @default.
• W2169283404 hasRelatedWork W1975032155 @default.
• W2169283404 hasRelatedWork W1996714420 @default.
• W2169283404 hasRelatedWork W2081573568 @default.
• W2169283404 hasRelatedWork W2352826163 @default.
• W2169283404 hasRelatedWork W2360482012 @default.
• W2169283404 hasRelatedWork W2362933455 @default.
• W2169283404 hasRelatedWork W2364959409 @default.
• W2169283404 hasRelatedWork W2367063708 @default.
• W2169283404 hasRelatedWork W2383357664 @default.
• W2169283404 hasRelatedWork W2903284256 @default.

• next