FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169359706> ?p ?o ?g. }

• W2169359706 abstract "Small-bodied freshwater fish are commonly used in regulatory testing for endocrine disrupting compounds (EDCs) but most lack a sensitive and quantifiable androgen-specific biomarker. Brook stickleback (Culaea inconstans) are a North American freshwater fish whose males produce an androgen-regulated glycoprotein in the kidney called spiggin. Although spiggin induction in females has been used as an androgen-specific biomarker of exposure in other stickleback species it has not been characterized in brook stickleback. Therefore, our objective was to develop a bioassay using brook stickleback to measure estrogenic and androgenic responses and establish the sensitivity of traditional and novel biomarkers of exposure. We first developed and optimized a qPCR assay to measure spiggin and vitellogenin transcript levels in kidney and liver tissue, respectively. Basal levels were differentially expressed in mature wild-caught male and female brook stickleback. To determine their sensitivity to EDCs, fish were exposed to nominal concentrations of 1, 10 and 100ng/L of 17α-methyltestosterone (MT) or 17α-ethinylestradiol (EE2) for 21days (sampled at 7 and 21days) under semi-static renewal conditions. MT and EE2 exposure induced spiggin and vitellogenin transcripts in female kidneys and male livers, respectively. Exposure to EE2 also increased hepatosomatic index in both sexes and decreased gonadosomatic index in females. Histopathological alterations were observed in the kidney of EE2-exposed fish and an increase in kidney epithelium cell height occurred in MT-exposed females. Given the sensitivity of these endpoints, the brook stickleback is a promising new freshwater fish model for EDC evaluation and a potential bioindicator for EDCs in North American freshwater environments." @default.
• W2169359706 created "2016-06-24" @default.
• W2169359706 creator A5053723634 @default.
• W2169359706 creator A5087052555 @default.
• W2169359706 date "2016-02-01" @default.
• W2169359706 modified "2023-09-24" @default.
• W2169359706 title "Biomarker responses to estrogen and androgen exposure in the brook stickleback (Culaea inconstans): A new bioindicator species for endocrine disrupting compounds" @default.
• W2169359706 cites W1586027995 @default.
• W2169359706 cites W1964266781 @default.
• W2169359706 cites W1968766422 @default.
• W2169359706 cites W1972630755 @default.
• W2169359706 cites W1973885169 @default.
• W2169359706 cites W1976705881 @default.
• W2169359706 cites W1978102412 @default.
• W2169359706 cites W1981420434 @default.
• W2169359706 cites W1982690673 @default.
• W2169359706 cites W1985666350 @default.
• W2169359706 cites W1994780605 @default.
• W2169359706 cites W1996850472 @default.
• W2169359706 cites W1998320916 @default.
• W2169359706 cites W2001252079 @default.
• W2169359706 cites W2006797518 @default.
• W2169359706 cites W2009409294 @default.
• W2169359706 cites W2012912649 @default.
• W2169359706 cites W2015036377 @default.
• W2169359706 cites W2026488872 @default.
• W2169359706 cites W2031179297 @default.
• W2169359706 cites W2039122086 @default.
• W2169359706 cites W2039420003 @default.
• W2169359706 cites W2044959308 @default.
• W2169359706 cites W2045516972 @default.
• W2169359706 cites W2054106173 @default.
• W2169359706 cites W2056663411 @default.
• W2169359706 cites W2056915102 @default.
• W2169359706 cites W2059883297 @default.
• W2169359706 cites W2062115282 @default.
• W2169359706 cites W2062403446 @default.
• W2169359706 cites W2063077146 @default.
• W2169359706 cites W2065899361 @default.
• W2169359706 cites W2074183900 @default.
• W2169359706 cites W2076223439 @default.
• W2169359706 cites W2079695346 @default.
• W2169359706 cites W2090778700 @default.
• W2169359706 cites W2093137134 @default.
• W2169359706 cites W2100153828 @default.
• W2169359706 cites W2100623909 @default.
• W2169359706 cites W2102045566 @default.
• W2169359706 cites W2119621367 @default.
• W2169359706 cites W2123175072 @default.
• W2169359706 cites W2126914119 @default.
• W2169359706 cites W2129106173 @default.
• W2169359706 cites W2129925090 @default.
• W2169359706 cites W2134034608 @default.
• W2169359706 cites W2137368494 @default.
• W2169359706 cites W2139631347 @default.
• W2169359706 cites W2143251305 @default.
• W2169359706 cites W2143853738 @default.
• W2169359706 cites W2144542356 @default.
• W2169359706 cites W2149199417 @default.
• W2169359706 cites W2153201071 @default.
• W2169359706 cites W2160109681 @default.
• W2169359706 cites W2169412126 @default.
• W2169359706 cites W2170796164 @default.
• W2169359706 cites W2325100872 @default.
• W2169359706 cites W4252030322 @default.
• W2169359706 cites W4252569230 @default.
• W2169359706 cites W69722208 @default.
• W2169359706 doi "https://doi.org/10.1016/j.cbpc.2015.10.013" @default.
• W2169359706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26545489" @default.
• W2169359706 hasPublicationYear "2016" @default.
• W2169359706 type Work @default.
• W2169359706 sameAs 2169359706 @default.
• W2169359706 citedByCount "5" @default.
• W2169359706 countsByYear W21693597062016 @default.
• W2169359706 countsByYear W21693597062017 @default.
• W2169359706 countsByYear W21693597062020 @default.
• W2169359706 countsByYear W21693597062021 @default.
• W2169359706 crossrefType "journal-article" @default.
• W2169359706 hasAuthorship W2169359706A5053723634 @default.
• W2169359706 hasAuthorship W2169359706A5087052555 @default.
• W2169359706 hasConcept C126322002 @default.
• W2169359706 hasConcept C134018914 @default.
• W2169359706 hasConcept C2777911890 @default.
• W2169359706 hasConcept C2778938320 @default.
• W2169359706 hasConcept C2779078696 @default.
• W2169359706 hasConcept C2779376097 @default.
• W2169359706 hasConcept C2780558290 @default.
• W2169359706 hasConcept C2780862370 @default.
• W2169359706 hasConcept C2781197716 @default.
• W2169359706 hasConcept C2908647359 @default.
• W2169359706 hasConcept C2909208804 @default.
• W2169359706 hasConcept C46699223 @default.
• W2169359706 hasConcept C505870484 @default.
• W2169359706 hasConcept C55493867 @default.
• W2169359706 hasConcept C67283656 @default.
• W2169359706 hasConcept C71315377 @default.
• W2169359706 hasConcept C71924100 @default.
• W2169359706 hasConcept C86803240 @default.
• W2169359706 hasConcept C90856448 @default.
• W2169359706 hasConcept C99454951 @default.

• next