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• W2169369815 abstract "Treatment of cells with DNA damaging agents leads to induction of a variety of genes involved in different cellular processes. We have applied a lacZ -based gene trap strategy to search for new mammalian genes induced by genotoxic stress. A population of 32 × 10 3neor clones stably transfected with a gene trap vector was obtained, stained with fluorescein di-β-D-galactopyranoside and analyzed by flow activated cell sorting and replica plating. This strategy allowed isolation of 30 neor ‘putative inducible’ cell lines expressing lacZ only after a DNA damaging treatment. For three clones the site of integration and the degree of inducibility after UV treatment were determined by Southern blot and β-galactosidase measurement respectively. One cell line (clone VI) showed a single integration site and a reproducible 3-fold induction of β-galactosidase activity following UV irradiation. Fused transcripts were isolated from induced cells and a portion of the trapped gene was amplified by rapid amplification of cDNA ends. Sequence analysis and comparison with available gene and protein databanks revealed that the gene was novel." @default.
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• W2169369815 date "1997-12-01" @default.
• W2169369815 modified "2023-09-25" @default.
• W2169369815 title "A gene trap approach to isolate mammalian genes involved in the cellular response to genotoxic stress" @default.
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• W2169369815 doi "https://doi.org/10.1093/nar/25.23.4803" @default.
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