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• W2169376598 abstract "The in vivo measurement of metabolic flux by 13 C-based metabolic flux analysis ( 13 C-MFA) provides valuable information regarding cell physiology. Bioinformatics tools have been developed to estimate metabolic flux distributions from the results of tracer isotopic labeling experiments using a 13 C-labeled carbon source. Metabolic flux is determined by nonlinear fitting of a metabolic model to the isotopic labeling enrichment of intracellular metabolites measured by mass spectrometry. Whereas 13 C-MFA is conventionally performed under isotopically constant conditions, isotopically nonstationary 13 C metabolic flux analysis (INST- 13 C-MFA) has recently been developed for flux analysis of cells with photosynthetic activity and cells at a quasi-steady metabolic state (e.g., primary cells or microorganisms under stationary phase). Here, the development of a novel open source software for INST- 13 C-MFA on the Windows platform is reported. OpenMebius (Open source software for Metabolic flux analysis) provides the function of autogenerating metabolic models for simulating isotopic labeling enrichment from a user-defined configuration worksheet. Analysis using simulated data demonstrated the applicability of OpenMebius for INST- 13 C-MFA. Confidence intervals determined by INST- 13 C-MFA were less than those determined by conventional methods, indicating the potential of INST- 13 C-MFA for precise metabolic flux analysis. OpenMebius is the open source software for the general application of INST- 13 C-MFA." @default.
• W2169376598 created "2016-06-24" @default.
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• W2169376598 date "2014-01-01" @default.
• W2169376598 modified "2023-09-25" @default.
• W2169376598 title "OpenMebius: An Open Source Software for Isotopically Nonstationary¹³C-Based Metabolic Flux Analysis" @default.
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• W2169376598 doi "https://doi.org/10.1155/2014/627014" @default.
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