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- W2169455209 abstract "Abstract In field studies of plant–insect herbivore interactions it is often difficult to establish which herbivore has fed on a particular plant. We investigated the suitability of three different 15 N‐labeled nitrogen compounds (ammonium, nitrate, and glycine) for indirect marking of three grasshopper species [ Omocestus viridulus (L.), Chorthippus parallelus (Zett.), and Chorthippus biguttulus (L.) (Orthoptera: Acrididae)] through labeling their food plants in the field. In two short‐term experiments grassland plots of 1 m 2 were separately labeled with either one of the different nitrogen compounds. Grasshoppers were caged on three food‐plant species [ Dactylis glomerata L., Holcus lanatus L. (Poaceae), and Trifolium repens L. (Fabaceae)] present in these plots for 72 h. Significantly enriched δ 15 N values in grasshoppers were found in all plant/grasshopper combinations. Enrichment in grasshoppers was positively correlated with the enrichment of plants and labeling with nitrate resulted in highest 15 N enrichment. In a long‐term experiment, individuals of C. biguttulus were placed in a cage covering an area of 1 m 2 for 37 days, with sampling of grasshoppers at regular intervals. δ 15 N values of the grasshopper and a common food plant, D. glomerata, increased steadily over time, up to 40‐fold by the end of the experiment. Our results demonstrate that 15 N‐labeling of plants is an appropriate tool for the investigation of insect–plant interactions under natural conditions." @default.
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- W2169455209 date "2005-08-15" @default.
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- W2169455209 title "Testing the efficiency of three 15N-labeled nitrogen compounds for indirect labeling of grasshoppers via plants in the field" @default.
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- W2169455209 doi "https://doi.org/10.1111/j.1570-7458.2005.00327.x" @default.
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