FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169558257> ?p ?o ?g. }

• W2169558257 abstract "Hypoxia Inducible Factor (HIF) regulates a cascade of transcriptional events in response to decreased oxygenation, acting from the cellular to the physiological level. This response is evolutionarily conserved, allowing the use of zebrafish (Danio rerio) as a model for studying the hypoxic response. Activation of the hypoxic response can be achieved in zebrafish by homozygous null mutation of the von Hippel-Lindau (vhl) tumour suppressor gene. Previous work from our lab has focused on the phenotypic characterisation of this mutant, establishing the links between vhl mutation, the hypoxic response and cancer. To further develop fish as a model for studying hypoxic signalling, we examine the transcriptional profile of the vhl mutant with respect to Hif-1α. As our approach uses embryos consisting of many cell types, it has the potential to uncover additional HIF regulated genes that have escaped detection in analogous mammalian cell culture studies. We performed high-density oligonucleotide microarray analysis of the gene expression changes in von Hippel-Lindau mutant zebrafish, which identified up-regulation of well-known hypoxia response genes and down-regulation of genes primarily involved in lipid processing. To identify the dependency of these transcriptional changes on HIF, we undertook Chromatin Immunoprecipitation linked next generation sequencing (ChIP-seq) for the transcription factor Hypoxia Inducible Factor 1α (HIF-1α). We identified HIF-1α binding sites across the genome, with binding sites showing enrichment for an RCGTG motif, showing conservation with the mammalian hypoxia response element. Transcriptome analysis of vhl mutant embryos detected activation of key hypoxia response genes seen in human cell models of hypoxia, but also suppression of many genes primarily involved in lipid processing. ChIP-seq analysis of Hif-1α binding sites unveiled an unprecedented number of loci, with a high proportion containing a canonical hypoxia response element. Whether these sites are functional remains unknown, nevertheless their frequent location near transcriptional start sites suggests functionality, and will allow for investigation into the potential hypoxic regulation of genes in their vicinity. We expect that our data will be an excellent starting point for analysis of both fish and mammalian gene regulation by HIF." @default.
• W2169558257 created "2016-06-24" @default.
• W2169558257 creator A5001706384 @default.
• W2169558257 creator A5042903453 @default.
• W2169558257 creator A5043126763 @default.
• W2169558257 creator A5047153002 @default.
• W2169558257 creator A5057418855 @default.
• W2169558257 creator A5063068496 @default.
• W2169558257 date "2015-11-11" @default.
• W2169558257 modified "2023-10-07" @default.
• W2169558257 title "Genome-wide mapping of Hif-1α binding sites in zebrafish" @default.
• W2169558257 cites W1597321356 @default.
• W2169558257 cites W1749022239 @default.
• W2169558257 cites W1965408671 @default.
• W2169558257 cites W1965827991 @default.
• W2169558257 cites W1969697457 @default.
• W2169558257 cites W1969734439 @default.
• W2169558257 cites W1976288322 @default.
• W2169558257 cites W1990607537 @default.
• W2169558257 cites W1996715681 @default.
• W2169558257 cites W1999670368 @default.
• W2169558257 cites W2000457934 @default.
• W2169558257 cites W2003764772 @default.
• W2169558257 cites W2005868568 @default.
• W2169558257 cites W2009085603 @default.
• W2169558257 cites W2009175965 @default.
• W2169558257 cites W2010160635 @default.
• W2169558257 cites W2010394923 @default.
• W2169558257 cites W2011076742 @default.
• W2169558257 cites W2013444425 @default.
• W2169558257 cites W2015644147 @default.
• W2169558257 cites W2016160962 @default.
• W2169558257 cites W2020968632 @default.
• W2169558257 cites W2027705428 @default.
• W2169558257 cites W2031778182 @default.
• W2169558257 cites W2033781392 @default.
• W2169558257 cites W2033803279 @default.
• W2169558257 cites W2033958147 @default.
• W2169558257 cites W2036822611 @default.
• W2169558257 cites W2037429185 @default.
• W2169558257 cites W2054987125 @default.
• W2169558257 cites W2058412949 @default.
• W2169558257 cites W2063076489 @default.
• W2169558257 cites W2063669037 @default.
• W2169558257 cites W2070007868 @default.
• W2169558257 cites W2071270368 @default.
• W2169558257 cites W2071278881 @default.
• W2169558257 cites W2075054245 @default.
• W2169558257 cites W2075706344 @default.
• W2169558257 cites W2079121632 @default.
• W2169558257 cites W2081098333 @default.
• W2169558257 cites W2085609942 @default.
• W2169558257 cites W2087161387 @default.
• W2169558257 cites W2088499209 @default.
• W2169558257 cites W2089346177 @default.
• W2169558257 cites W2090627731 @default.
• W2169558257 cites W2095793094 @default.
• W2169558257 cites W2097569535 @default.
• W2169558257 cites W2098134455 @default.
• W2169558257 cites W2098902167 @default.
• W2169558257 cites W2099070401 @default.
• W2169558257 cites W2099117463 @default.
• W2169558257 cites W2100649970 @default.
• W2169558257 cites W2101511754 @default.
• W2169558257 cites W2106133610 @default.
• W2169558257 cites W2108244474 @default.
• W2169558257 cites W2110015307 @default.
• W2169558257 cites W2118030557 @default.
• W2169558257 cites W2118148854 @default.
• W2169558257 cites W2125505202 @default.
• W2169558257 cites W2127255821 @default.
• W2169558257 cites W2131473358 @default.
• W2169558257 cites W2132869125 @default.
• W2169558257 cites W2133465414 @default.
• W2169558257 cites W2133664945 @default.
• W2169558257 cites W2134476131 @default.
• W2169558257 cites W2139543010 @default.
• W2169558257 cites W2140607197 @default.
• W2169558257 cites W2144625075 @default.
• W2169558257 cites W2146512944 @default.
• W2169558257 cites W2149783560 @default.
• W2169558257 cites W2149870009 @default.
• W2169558257 cites W2153899459 @default.
• W2169558257 cites W2157705760 @default.
• W2169558257 cites W2158217645 @default.
• W2169558257 cites W2158435332 @default.
• W2169558257 cites W2158821085 @default.
• W2169558257 cites W2161216917 @default.
• W2169558257 cites W2164216268 @default.
• W2169558257 cites W2166777888 @default.
• W2169558257 cites W2168086696 @default.
• W2169558257 cites W2170009799 @default.
• W2169558257 cites W2171808845 @default.
• W2169558257 doi "https://doi.org/10.1186/s12864-015-2169-x" @default.
• W2169558257 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4642629" @default.
• W2169558257 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26559940" @default.
• W2169558257 hasPublicationYear "2015" @default.
• W2169558257 type Work @default.
• W2169558257 sameAs 2169558257 @default.
• W2169558257 citedByCount "29" @default.

• next