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• W2169577566 abstract "Fission yeast centromeric repeats are transcribed into small interfering RNA (siRNA) precursors (pre-siRNAs), which are processed by Dicer to direct heterochromatin formation. Recently, Rpb1 and Rpb2 subunits of RNA polymerase II (RNA Pol II) were shown to mediate RNA interference (RNAi)-directed chromatin modification but did not affect pre-siRNA levels. Here we show that another Pol II subunit, Rpb7 has a specific role in pre-siRNA transcription. We define a centromeric pre-siRNA promoter from which initiation is exquisitely sensitive to the rpb7-G150D mutation. In contrast to other Pol II subunits, Rpb7 promotes pre-siRNA transcription required for RNAi-directed chromatin silencing." @default.
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• W2169577566 date "2005-10-01" @default.
• W2169577566 modified "2023-10-12" @default.
• W2169577566 title "RNA Pol II subunit Rpb7 promotes centromeric transcription and RNAi-directed chromatin silencing" @default.
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• W2169577566 doi "https://doi.org/10.1101/gad.344205" @default.
• W2169577566 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1240039" @default.
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