FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169581649> ?p ?o ?g. }

• W2169581649 endingPage "1539" @default.
• W2169581649 startingPage "1539" @default.
• W2169581649 abstract "Calla lilies are ornamental plants of major economic importance in Taiwan. They are grown in the central and northern areas of the island, and ≈3 million stems are shipped annually. Calla lilies are susceptible to several viruses (1). Infections by Cucumber mosaic virus, Dasheen mosaic virus, Turnip mosaic virus, and Watermelon silver mottle virus were reported in Taiwan. Recently, virus-like symptoms including yellow mottling, light yellow spot, yellow ringspot, and mosaic were observed on leaves of field-grown calla lilies from Changhua County, located in central Taiwan. In March 2001, a virus culture was isolated from diseased calla lilies and established in Chenopodium quinoa Willd. and Nicotiana benthamiana Domin. When inoculated with the virus, healthy calla lilies developed chlorotic spots that enlarged and fused to form large, yellow patches on inoculated leaves. Symptoms were similar to those on the naturally infected plants observed in the fields. The virus induced chlorotic local lesions on C. quinoa, C. ficifolium Sm., C. amaranticolor Coste & Reyn, Cucurbita moschata Duchesne ex Poir, Lisianthus russellianum (Don.) Griseb, Phaseolus angularis Wight, Vigna angularis Willd., and V. radiata (L.) Wilczek. In addition to the localized chlorotic spots on inoculated leaves, systemic invasion of the virus was also observed 8 to 10 days postinoculation in Dianthus caryophyllus L., D. chinensis L., and Glycine max Merr. In N. benthamiana, the only symptom observed was systemic wilting. Examination of 2% of uranyl-acetate-stained samples using electron microscopy revealed the presence of spherical particles ≈34 to 35 nm in diameter in crude extracts of leaves of diseased calla lilies, or infected C. quinoa. Similar particles were also observed in the cytoplasm but not in the nuclei in ultrathin sections of virus-infected leaf tissues of C. quinoa and N. benthamiana. Differential centrifugation followed by sucrose density gradient centrifugation of tissue extracts of infected C. quinoa yielded virions with similar size. Sodium dodesyl sulfate polyacrylamide gel electrophoresis of the purified virus showed a single structural polypeptide ith a M r of 41.6 kDa. The viral antigen reacted positively with its homologous antiserum and an antiserum against Carnation mottle virus (CarMV; Agdia, Inc., Elkhart, IN) in double antibody sandwich enzyme-linked immunosorbent assay. Using primers 5′-CTCCATGGTCATGGAA(A/G)ATAAA GGAGAA and 3′-CAACAAATATCCTACACTGTCCTAGGTG specific to the coat protein (CP) gene of CarMV, an expected viral CP gene product of 1.05 kb was amplified by reverse transcription-polymerase chain reaction from total RNA isolated from infected N. benthamiana. Comparisons of the 1,047-nucleotide CP gene with those of 15 CarMV isolates available in GenBank showed 94.6 to 98.2% nucleotide identity and 94.8 to 96.8% amino acid identity. Results from current studies indicate that the virus infecting calla lilies is an isolate of CarMV. To our knowledge, this is the first report of CarMV infection in calla lilies. The occurrence of CarMV in calla lilies has direct implication for the economically important nursery and floral industry in Taiwan. Reference: (1) F. W. Zettler and R. D. Hartman. Dieffenbachia, Caladium, and Zantedeschia. Pages 464–470 in: Virus and Virus-Like Diseases of Bulb and Flower Crops. G. Loebenstein, R. H. Lawson, and A. A. Brunt, eds. John Wiley and Sons, West Sussex, U.K., 1995." @default.
• W2169581649 created "2016-06-24" @default.
• W2169581649 creator A5027053389 @default.
• W2169581649 creator A5028153449 @default.
• W2169581649 creator A5038351003 @default.
• W2169581649 creator A5046467160 @default.
• W2169581649 creator A5053206821 @default.
• W2169581649 date "2003-12-01" @default.
• W2169581649 modified "2023-10-13" @default.
• W2169581649 title "First Report of <i>Carnation mottle virus</i> in Calla lily (<i>Zantedeschia</i> spp.)" @default.
• W2169581649 doi "https://doi.org/10.1094/pdis.2003.87.12.1539c" @default.
• W2169581649 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30812409" @default.
• W2169581649 hasPublicationYear "2003" @default.
• W2169581649 type Work @default.
• W2169581649 sameAs 2169581649 @default.
• W2169581649 citedByCount "9" @default.
• W2169581649 countsByYear W21695816492013 @default.
• W2169581649 countsByYear W21695816492014 @default.
• W2169581649 countsByYear W21695816492015 @default.
• W2169581649 countsByYear W21695816492017 @default.
• W2169581649 countsByYear W21695816492019 @default.
• W2169581649 countsByYear W21695816492022 @default.
• W2169581649 crossrefType "journal-article" @default.
• W2169581649 hasAuthorship W2169581649A5027053389 @default.
• W2169581649 hasAuthorship W2169581649A5028153449 @default.
• W2169581649 hasAuthorship W2169581649A5038351003 @default.
• W2169581649 hasAuthorship W2169581649A5046467160 @default.
• W2169581649 hasAuthorship W2169581649A5053206821 @default.
• W2169581649 hasConcept C109110057 @default.
• W2169581649 hasConcept C144027150 @default.
• W2169581649 hasConcept C159047783 @default.
• W2169581649 hasConcept C159654299 @default.
• W2169581649 hasConcept C180032290 @default.
• W2169581649 hasConcept C203014093 @default.
• W2169581649 hasConcept C2522874641 @default.
• W2169581649 hasConcept C2775891814 @default.
• W2169581649 hasConcept C2776513250 @default.
• W2169581649 hasConcept C2777820912 @default.
• W2169581649 hasConcept C2778415121 @default.
• W2169581649 hasConcept C2780562374 @default.
• W2169581649 hasConcept C2993082939 @default.
• W2169581649 hasConcept C542903549 @default.
• W2169581649 hasConcept C59822182 @default.
• W2169581649 hasConcept C74103781 @default.
• W2169581649 hasConcept C86803240 @default.
• W2169581649 hasConceptScore W2169581649C109110057 @default.
• W2169581649 hasConceptScore W2169581649C144027150 @default.
• W2169581649 hasConceptScore W2169581649C159047783 @default.
• W2169581649 hasConceptScore W2169581649C159654299 @default.
• W2169581649 hasConceptScore W2169581649C180032290 @default.
• W2169581649 hasConceptScore W2169581649C203014093 @default.
• W2169581649 hasConceptScore W2169581649C2522874641 @default.
• W2169581649 hasConceptScore W2169581649C2775891814 @default.
• W2169581649 hasConceptScore W2169581649C2776513250 @default.
• W2169581649 hasConceptScore W2169581649C2777820912 @default.
• W2169581649 hasConceptScore W2169581649C2778415121 @default.
• W2169581649 hasConceptScore W2169581649C2780562374 @default.
• W2169581649 hasConceptScore W2169581649C2993082939 @default.
• W2169581649 hasConceptScore W2169581649C542903549 @default.
• W2169581649 hasConceptScore W2169581649C59822182 @default.
• W2169581649 hasConceptScore W2169581649C74103781 @default.
• W2169581649 hasConceptScore W2169581649C86803240 @default.
• W2169581649 hasIssue "12" @default.
• W2169581649 hasLocation W21695816491 @default.
• W2169581649 hasLocation W21695816492 @default.
• W2169581649 hasOpenAccess W2169581649 @default.
• W2169581649 hasPrimaryLocation W21695816491 @default.
• W2169581649 hasRelatedWork W1957602806 @default.
• W2169581649 hasRelatedWork W2009110608 @default.
• W2169581649 hasRelatedWork W2020750486 @default.
• W2169581649 hasRelatedWork W2033762348 @default.
• W2169581649 hasRelatedWork W2050992416 @default.
• W2169581649 hasRelatedWork W2077796902 @default.
• W2169581649 hasRelatedWork W2321055576 @default.
• W2169581649 hasRelatedWork W2979095783 @default.
• W2169581649 hasRelatedWork W2990706406 @default.
• W2169581649 hasRelatedWork W3092492335 @default.
• W2169581649 hasVolume "87" @default.
• W2169581649 isParatext "false" @default.
• W2169581649 isRetracted "false" @default.
• W2169581649 magId "2169581649" @default.
• W2169581649 workType "article" @default.