Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169650147> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2169650147 endingPage "431" @default.
- W2169650147 startingPage "428" @default.
- W2169650147 abstract "The often-encountered problem of disrupting bacteria for the purpose of extracting soluble protein has generated various methods. Many require specialized equipment. Very often, especially during preliminary studies, investigators need a simple, fast, and inexpensive method for cell disruption that preserves biological activity. This paper compares some simple and inexpensive methods for cell disruption, such as bead-vortexing, freezing-thawing, French pressing, and sonication. It also provides some tips to increase protein yield and preserve biological activity. If performed under optimal conditions, bead-vortexing gives protein yields that are comparable to French pressing and sonication. It also preserves the activities of labile enzymes and releases periplasmic enzymes. Vortexing with glass beads appears to be the simplest method for cell disruption." @default.
- W2169650147 created "2016-06-24" @default.
- W2169650147 creator A5009276853 @default.
- W2169650147 creator A5085808655 @default.
- W2169650147 date "2002-07-31" @default.
- W2169650147 modified "2023-10-18" @default.
- W2169650147 title "Disrupting Escherichia coli: A Comparison of Methods" @default.
- W2169650147 cites W1965121007 @default.
- W2169650147 cites W1989801923 @default.
- W2169650147 cites W1990264387 @default.
- W2169650147 cites W2122486223 @default.
- W2169650147 doi "https://doi.org/10.5483/bmbrep.2002.35.4.428" @default.
- W2169650147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12297004" @default.
- W2169650147 hasPublicationYear "2002" @default.
- W2169650147 type Work @default.
- W2169650147 sameAs 2169650147 @default.
- W2169650147 citedByCount "37" @default.
- W2169650147 countsByYear W21696501472012 @default.
- W2169650147 countsByYear W21696501472013 @default.
- W2169650147 countsByYear W21696501472014 @default.
- W2169650147 countsByYear W21696501472015 @default.
- W2169650147 countsByYear W21696501472016 @default.
- W2169650147 countsByYear W21696501472017 @default.
- W2169650147 countsByYear W21696501472018 @default.
- W2169650147 countsByYear W21696501472019 @default.
- W2169650147 countsByYear W21696501472021 @default.
- W2169650147 countsByYear W21696501472022 @default.
- W2169650147 crossrefType "journal-article" @default.
- W2169650147 hasAuthorship W2169650147A5009276853 @default.
- W2169650147 hasAuthorship W2169650147A5085808655 @default.
- W2169650147 hasBestOaLocation W21696501471 @default.
- W2169650147 hasConcept C104317684 @default.
- W2169650147 hasConcept C104628117 @default.
- W2169650147 hasConcept C109386097 @default.
- W2169650147 hasConcept C127413603 @default.
- W2169650147 hasConcept C134121241 @default.
- W2169650147 hasConcept C159985019 @default.
- W2169650147 hasConcept C183696295 @default.
- W2169650147 hasConcept C185592680 @default.
- W2169650147 hasConcept C191897082 @default.
- W2169650147 hasConcept C192562407 @default.
- W2169650147 hasConcept C201663137 @default.
- W2169650147 hasConcept C26268613 @default.
- W2169650147 hasConcept C43617362 @default.
- W2169650147 hasConcept C523546767 @default.
- W2169650147 hasConcept C54355233 @default.
- W2169650147 hasConcept C547475151 @default.
- W2169650147 hasConcept C55493867 @default.
- W2169650147 hasConcept C86803240 @default.
- W2169650147 hasConcept C93021684 @default.
- W2169650147 hasConceptScore W2169650147C104317684 @default.
- W2169650147 hasConceptScore W2169650147C104628117 @default.
- W2169650147 hasConceptScore W2169650147C109386097 @default.
- W2169650147 hasConceptScore W2169650147C127413603 @default.
- W2169650147 hasConceptScore W2169650147C134121241 @default.
- W2169650147 hasConceptScore W2169650147C159985019 @default.
- W2169650147 hasConceptScore W2169650147C183696295 @default.
- W2169650147 hasConceptScore W2169650147C185592680 @default.
- W2169650147 hasConceptScore W2169650147C191897082 @default.
- W2169650147 hasConceptScore W2169650147C192562407 @default.
- W2169650147 hasConceptScore W2169650147C201663137 @default.
- W2169650147 hasConceptScore W2169650147C26268613 @default.
- W2169650147 hasConceptScore W2169650147C43617362 @default.
- W2169650147 hasConceptScore W2169650147C523546767 @default.
- W2169650147 hasConceptScore W2169650147C54355233 @default.
- W2169650147 hasConceptScore W2169650147C547475151 @default.
- W2169650147 hasConceptScore W2169650147C55493867 @default.
- W2169650147 hasConceptScore W2169650147C86803240 @default.
- W2169650147 hasConceptScore W2169650147C93021684 @default.
- W2169650147 hasIssue "4" @default.
- W2169650147 hasLocation W21696501471 @default.
- W2169650147 hasLocation W21696501472 @default.
- W2169650147 hasOpenAccess W2169650147 @default.
- W2169650147 hasPrimaryLocation W21696501471 @default.
- W2169650147 hasRelatedWork W1971075660 @default.
- W2169650147 hasRelatedWork W1979061422 @default.
- W2169650147 hasRelatedWork W2124791093 @default.
- W2169650147 hasRelatedWork W2389526001 @default.
- W2169650147 hasRelatedWork W2742791959 @default.
- W2169650147 hasRelatedWork W2808518912 @default.
- W2169650147 hasRelatedWork W2822351394 @default.
- W2169650147 hasRelatedWork W2925365648 @default.
- W2169650147 hasRelatedWork W3014995365 @default.
- W2169650147 hasRelatedWork W4221049100 @default.
- W2169650147 hasVolume "35" @default.
- W2169650147 isParatext "false" @default.
- W2169650147 isRetracted "false" @default.
- W2169650147 magId "2169650147" @default.
- W2169650147 workType "article" @default.