FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169667134> ?p ?o ?g. }

• W2169667134 endingPage "e18704" @default.
• W2169667134 startingPage "e18704" @default.
• W2169667134 abstract "Normal urinary bladder function requires bidirectional molecular communication between urothelium, detrusor smooth muscle and sensory neurons and one of the key mediators involved in this intercellular signaling is ATP. Ectonucleotidases dephosphorylate nucleotides and thus regulate ligand exposure to P2X and P2Y purinergic receptors. Little is known about the role of these enzymes in mammalian bladder despite substantial literature linking bladder diseases to aberrant purinergic signaling. We therefore examined the expression and distribution of ectonucleotidases in the mouse bladder since mice offer the advantage of straightforward genetic modification for future studies.RT-PCR demonstrated that eight members of the ectonucleoside triphosphate diphosphohydrolase (NTPD) family, as well as 5'-nucleotidase (NT5E) are expressed in mouse bladder. NTPD1, NTPD2, NTPD3, NTPD8 and NT5E all catalyze extracellular nucleotide dephosphorylation and in concert achieve stepwise conversion of extracellular ATP to adenosine. Immunofluorescent localization with confocal microscopy revealed NTPD1 in endothelium of blood vessels in the lamina propria and in detrusor smooth muscle cells, while NTPD2 was expressed in cells localized to a region of the lamina propria adjacent to detrusor and surrounding muscle bundles in the detrusor. NTPD3 was urothelial-specific, occurring on membranes of intermediate and basal epithelial cells but did not appear to be present in umbrella cells. Immunoblotting confirmed NTPD8 protein in bladder and immunofluorescence suggested a primary localization to the urothelium. NT5E was present exclusively in detrusor smooth muscle in a pattern complementary with that of NTPD1 suggesting a mechanism for providing adenosine to P1 receptors on the surface of myocytes.Ectonucleotidases exhibit highly cell-specific expression patterns in bladder and therefore likely act in a coordinated manner to regulate ligand availability to purinergic receptors. This is the first study to determine the expression and location of ectonucleotidases within the mammalian urinary bladder." @default.
• W2169667134 created "2016-06-24" @default.
• W2169667134 creator A5041621533 @default.
• W2169667134 creator A5060216484 @default.
• W2169667134 creator A5087929660 @default.
• W2169667134 date "2011-04-14" @default.
• W2169667134 modified "2023-09-25" @default.
• W2169667134 title "Expression and Distribution of Ectonucleotidases in Mouse Urinary Bladder" @default.
• W2169667134 cites W1565285014 @default.
• W2169667134 cites W1575614472 @default.
• W2169667134 cites W1581192104 @default.
• W2169667134 cites W1623121663 @default.
• W2169667134 cites W1767633497 @default.
• W2169667134 cites W1858172004 @default.
• W2169667134 cites W1963639903 @default.
• W2169667134 cites W1965914047 @default.
• W2169667134 cites W1980503559 @default.
• W2169667134 cites W1980721328 @default.
• W2169667134 cites W1981985054 @default.
• W2169667134 cites W1983509502 @default.
• W2169667134 cites W1984717720 @default.
• W2169667134 cites W1986755791 @default.
• W2169667134 cites W1991807289 @default.
• W2169667134 cites W1992371893 @default.
• W2169667134 cites W2006419030 @default.
• W2169667134 cites W2010783115 @default.
• W2169667134 cites W2023889413 @default.
• W2169667134 cites W2026423422 @default.
• W2169667134 cites W2028886123 @default.
• W2169667134 cites W2052986896 @default.
• W2169667134 cites W2053902290 @default.
• W2169667134 cites W2055604814 @default.
• W2169667134 cites W2057772772 @default.
• W2169667134 cites W2059965072 @default.
• W2169667134 cites W2063060988 @default.
• W2169667134 cites W2072024311 @default.
• W2169667134 cites W2073451266 @default.
• W2169667134 cites W2076617795 @default.
• W2169667134 cites W2077930885 @default.
• W2169667134 cites W2082021100 @default.
• W2169667134 cites W2083368511 @default.
• W2169667134 cites W2085789947 @default.
• W2169667134 cites W2089363276 @default.
• W2169667134 cites W2095988220 @default.
• W2169667134 cites W2099076586 @default.
• W2169667134 cites W2099689816 @default.
• W2169667134 cites W2101859335 @default.
• W2169667134 cites W2109675166 @default.
• W2169667134 cites W2113959251 @default.
• W2169667134 cites W2120534464 @default.
• W2169667134 cites W2123492226 @default.
• W2169667134 cites W2127651585 @default.
• W2169667134 cites W2134499166 @default.
• W2169667134 cites W2138664829 @default.
• W2169667134 cites W2139775312 @default.
• W2169667134 cites W2139978055 @default.
• W2169667134 cites W2144592553 @default.
• W2169667134 cites W2146264886 @default.
• W2169667134 cites W2148212424 @default.
• W2169667134 cites W2150371994 @default.
• W2169667134 cites W2158668257 @default.
• W2169667134 cites W2161777760 @default.
• W2169667134 cites W2164522021 @default.
• W2169667134 cites W2165730698 @default.
• W2169667134 doi "https://doi.org/10.1371/journal.pone.0018704" @default.
• W2169667134 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3077397" @default.
• W2169667134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21533188" @default.
• W2169667134 hasPublicationYear "2011" @default.
• W2169667134 type Work @default.
• W2169667134 sameAs 2169667134 @default.
• W2169667134 citedByCount "46" @default.
• W2169667134 countsByYear W21696671342012 @default.
• W2169667134 countsByYear W21696671342013 @default.
• W2169667134 countsByYear W21696671342014 @default.
• W2169667134 countsByYear W21696671342015 @default.
• W2169667134 countsByYear W21696671342016 @default.
• W2169667134 countsByYear W21696671342018 @default.
• W2169667134 countsByYear W21696671342019 @default.
• W2169667134 countsByYear W21696671342020 @default.
• W2169667134 countsByYear W21696671342021 @default.
• W2169667134 countsByYear W21696671342022 @default.
• W2169667134 countsByYear W21696671342023 @default.
• W2169667134 crossrefType "journal-article" @default.
• W2169667134 hasAuthorship W2169667134A5041621533 @default.
• W2169667134 hasAuthorship W2169667134A5060216484 @default.
• W2169667134 hasAuthorship W2169667134A5087929660 @default.
• W2169667134 hasBestOaLocation W21696671341 @default.
• W2169667134 hasConcept C126322002 @default.
• W2169667134 hasConcept C134018914 @default.
• W2169667134 hasConcept C160225129 @default.
• W2169667134 hasConcept C170493617 @default.
• W2169667134 hasConcept C172590197 @default.
• W2169667134 hasConcept C185592680 @default.
• W2169667134 hasConcept C2776991684 @default.
• W2169667134 hasConcept C2777310793 @default.
• W2169667134 hasConcept C2777540882 @default.
• W2169667134 hasConcept C2778938600 @default.
• W2169667134 hasConcept C2779762690 @default.

• next