Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169721738> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2169721738 endingPage "251" @default.
- W2169721738 startingPage "247" @default.
- W2169721738 abstract "The innate immune system uses Toll-like receptors (TLR) to detect the presence of pathogen patterns thus allowing for rapid host defense responses. Stimulation of TLR results in inflammatory response and regulatory cytokine production affecting acquired immunity. The aim of the study was an evaluation of TLR2 and TLR4 expression on the surface of human colon cancer cells in primary culture with or without autologous peripheral blood mononuclear cells. Surgical specimens of colon cancer were processed to obtain cancer cells. Cancer cells separation was conducted first by mechanical tissue disintegration and than by gradient centrifugation to obtain 95 % cell confluence. By staining the isolated cells the pathologist determined them as adenocarcinoma. Colon cancer cells were then co-cultured in 24 h culture alone or together with autologous lymphocytes. Reverse-transcription polymerase chain reaction was performed for detection of TLR2 and TLR4 mRNA in colon cancer and normal colon epithelial cells using commercially available primers. Resting as well as phytohemagglutinin or lipopolysaccharide (LPS) stimulated cells were tested. Receptor proteins on cancer cells were examined by immunohistochemistry. TLR4 mRNA was detected in cancer cells. Autologous lymphocytes do not exert any effect on these receptors expression. TLR4 mRNA expression was not observed in normal colon epithelial cells. TLR2 mRNA was present on LPS stimulated cancer cells as well as on resting and stimulated lymphocytes. Expression of TLR2 and TLR4 receptor proteins on colon cancer cells were confirmed by immunohistochemistry. TLR4 may be responsible for uncontrolled tumor growth under LPS stimulation in human colon environment." @default.
- W2169721738 created "2016-06-24" @default.
- W2169721738 creator A5036241194 @default.
- W2169721738 creator A5044000443 @default.
- W2169721738 creator A5044075217 @default.
- W2169721738 creator A5081197121 @default.
- W2169721738 date "2014-01-05" @default.
- W2169721738 modified "2023-10-07" @default.
- W2169721738 title "Expression of Toll-Like Receptors on Human Rectal Adenocarcinoma Cells" @default.
- W2169721738 cites W1982297066 @default.
- W2169721738 cites W2003639759 @default.
- W2169721738 cites W2023896765 @default.
- W2169721738 cites W2036519065 @default.
- W2169721738 cites W2037580272 @default.
- W2169721738 cites W2051198779 @default.
- W2169721738 cites W2052115777 @default.
- W2169721738 cites W2053043145 @default.
- W2169721738 cites W2054538838 @default.
- W2169721738 cites W2065054017 @default.
- W2169721738 cites W2073921028 @default.
- W2169721738 cites W2080128768 @default.
- W2169721738 cites W2095348902 @default.
- W2169721738 cites W2113769081 @default.
- W2169721738 cites W2121797213 @default.
- W2169721738 doi "https://doi.org/10.1007/s00005-013-0260-z" @default.
- W2169721738 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4024133" @default.
- W2169721738 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24390484" @default.
- W2169721738 hasPublicationYear "2014" @default.
- W2169721738 type Work @default.
- W2169721738 sameAs 2169721738 @default.
- W2169721738 citedByCount "6" @default.
- W2169721738 countsByYear W21697217382017 @default.
- W2169721738 countsByYear W21697217382018 @default.
- W2169721738 countsByYear W21697217382019 @default.
- W2169721738 crossrefType "journal-article" @default.
- W2169721738 hasAuthorship W2169721738A5036241194 @default.
- W2169721738 hasAuthorship W2169721738A5044000443 @default.
- W2169721738 hasAuthorship W2169721738A5044075217 @default.
- W2169721738 hasAuthorship W2169721738A5081197121 @default.
- W2169721738 hasBestOaLocation W21697217381 @default.
- W2169721738 hasConcept C121608353 @default.
- W2169721738 hasConcept C136449434 @default.
- W2169721738 hasConcept C142724271 @default.
- W2169721738 hasConcept C153911025 @default.
- W2169721738 hasConcept C170493617 @default.
- W2169721738 hasConcept C203014093 @default.
- W2169721738 hasConcept C2776070231 @default.
- W2169721738 hasConcept C2776709828 @default.
- W2169721738 hasConcept C2781182431 @default.
- W2169721738 hasConcept C2781236682 @default.
- W2169721738 hasConcept C502942594 @default.
- W2169721738 hasConcept C526805850 @default.
- W2169721738 hasConcept C54355233 @default.
- W2169721738 hasConcept C55493867 @default.
- W2169721738 hasConcept C71924100 @default.
- W2169721738 hasConcept C86803240 @default.
- W2169721738 hasConcept C8891405 @default.
- W2169721738 hasConcept C96232424 @default.
- W2169721738 hasConceptScore W2169721738C121608353 @default.
- W2169721738 hasConceptScore W2169721738C136449434 @default.
- W2169721738 hasConceptScore W2169721738C142724271 @default.
- W2169721738 hasConceptScore W2169721738C153911025 @default.
- W2169721738 hasConceptScore W2169721738C170493617 @default.
- W2169721738 hasConceptScore W2169721738C203014093 @default.
- W2169721738 hasConceptScore W2169721738C2776070231 @default.
- W2169721738 hasConceptScore W2169721738C2776709828 @default.
- W2169721738 hasConceptScore W2169721738C2781182431 @default.
- W2169721738 hasConceptScore W2169721738C2781236682 @default.
- W2169721738 hasConceptScore W2169721738C502942594 @default.
- W2169721738 hasConceptScore W2169721738C526805850 @default.
- W2169721738 hasConceptScore W2169721738C54355233 @default.
- W2169721738 hasConceptScore W2169721738C55493867 @default.
- W2169721738 hasConceptScore W2169721738C71924100 @default.
- W2169721738 hasConceptScore W2169721738C86803240 @default.
- W2169721738 hasConceptScore W2169721738C8891405 @default.
- W2169721738 hasConceptScore W2169721738C96232424 @default.
- W2169721738 hasIssue "3" @default.
- W2169721738 hasLocation W21697217381 @default.
- W2169721738 hasLocation W21697217382 @default.
- W2169721738 hasLocation W21697217383 @default.
- W2169721738 hasLocation W21697217384 @default.
- W2169721738 hasOpenAccess W2169721738 @default.
- W2169721738 hasPrimaryLocation W21697217381 @default.
- W2169721738 hasRelatedWork W1897355343 @default.
- W2169721738 hasRelatedWork W1914689199 @default.
- W2169721738 hasRelatedWork W1993663150 @default.
- W2169721738 hasRelatedWork W2029376832 @default.
- W2169721738 hasRelatedWork W2033318041 @default.
- W2169721738 hasRelatedWork W2047730062 @default.
- W2169721738 hasRelatedWork W2359392350 @default.
- W2169721738 hasRelatedWork W2399867088 @default.
- W2169721738 hasRelatedWork W2793472432 @default.
- W2169721738 hasRelatedWork W4206957485 @default.
- W2169721738 hasVolume "62" @default.
- W2169721738 isParatext "false" @default.
- W2169721738 isRetracted "false" @default.
- W2169721738 magId "2169721738" @default.
- W2169721738 workType "article" @default.