FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169733090> ?p ?o ?g. }

• W2169733090 endingPage "1277" @default.
• W2169733090 startingPage "1267" @default.
• W2169733090 abstract "A cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Spinacia oleracea leaf library and used to express a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kDa. Soluble protein was purified to homogeneity from S. frugiperda cells infected with recombinant baculovirus harboring the isolated cDNA. The soluble protein had a molecular mass of 320 kDa, estimated by gel filtration chromatography, and␣a␣subunit size of 90.8 kDa. The purified protein had␣activity of␣both 6-phosphofructo-2-kinase (specific␣activity 10.4–15.9 nmol·min−1·mg protein−1) and fructose-2,6-bisphosphatase (specific activity 1.65–1.75 nmol·min−1·mg protein−1). The 6-phosphofructo-2-kinase activity was activated by inorganic phosphate, and inhibited by 3-carbon phosphorylated metabolites and pyrophosphate. In the presence of phosphate, 3-phosphoglycerate was a mixed inhibitor with respect to both fructose 6-phosphate and ATP. Fructose-2,6-bisphosphatase activity was sensitive to product inhibition; inhibition by inorganic phosphate was uncompetitive, whereas inhibition by fructose 6-phosphate was mixed. These kinetic properties support the view that the level of fructose 2,6-bisphosphate in leaves is determined by the relative concentrations of hexose phosphates, three-carbon phosphate esters and inorganic phosphate in the cytosol through reciprocal modulation of 6-phosphofructo-2-kinase and fructose-2,6-bisphosphatase activities of the bifunctional enzyme." @default.
• W2169733090 created "2016-06-24" @default.
• W2169733090 creator A5024467630 @default.
• W2169733090 creator A5064104793 @default.
• W2169733090 date "2002-02-15" @default.
• W2169733090 modified "2023-09-27" @default.
• W2169733090 title "Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves" @default.
• W2169733090 cites W1508384599 @default.
• W2169733090 cites W1570064176 @default.
• W2169733090 cites W1596417761 @default.
• W2169733090 cites W1750662224 @default.
• W2169733090 cites W1968129408 @default.
• W2169733090 cites W1976462829 @default.
• W2169733090 cites W1988080353 @default.
• W2169733090 cites W1990133090 @default.
• W2169733090 cites W1991461352 @default.
• W2169733090 cites W1992485982 @default.
• W2169733090 cites W2000081372 @default.
• W2169733090 cites W2003805523 @default.
• W2169733090 cites W2004231513 @default.
• W2169733090 cites W2012841352 @default.
• W2169733090 cites W2013706919 @default.
• W2169733090 cites W2022186084 @default.
• W2169733090 cites W2025616008 @default.
• W2169733090 cites W2026051255 @default.
• W2169733090 cites W2035682770 @default.
• W2169733090 cites W2038163496 @default.
• W2169733090 cites W2058834003 @default.
• W2169733090 cites W2070669901 @default.
• W2169733090 cites W2087070363 @default.
• W2169733090 cites W2094691257 @default.
• W2169733090 cites W2123693761 @default.
• W2169733090 cites W2132293500 @default.
• W2169733090 cites W21404215 @default.
• W2169733090 cites W2140842418 @default.
• W2169733090 cites W2143917905 @default.
• W2169733090 cites W2146956832 @default.
• W2169733090 cites W2482068925 @default.
• W2169733090 cites W4293247451 @default.
• W2169733090 cites W832020001 @default.
• W2169733090 doi "https://doi.org/10.1046/j.1432-1033.2002.02771.x" @default.
• W2169733090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11856361" @default.
• W2169733090 hasPublicationYear "2002" @default.
• W2169733090 type Work @default.
• W2169733090 sameAs 2169733090 @default.
• W2169733090 citedByCount "20" @default.
• W2169733090 countsByYear W21697330902015 @default.
• W2169733090 countsByYear W21697330902018 @default.
• W2169733090 countsByYear W21697330902022 @default.
• W2169733090 countsByYear W21697330902023 @default.
• W2169733090 crossrefType "journal-article" @default.
• W2169733090 hasAuthorship W2169733090A5024467630 @default.
• W2169733090 hasAuthorship W2169733090A5064104793 @default.
• W2169733090 hasBestOaLocation W21697330901 @default.
• W2169733090 hasConcept C109110057 @default.
• W2169733090 hasConcept C122245378 @default.
• W2169733090 hasConcept C14728290 @default.
• W2169733090 hasConcept C150757390 @default.
• W2169733090 hasConcept C153911025 @default.
• W2169733090 hasConcept C159047783 @default.
• W2169733090 hasConcept C181199279 @default.
• W2169733090 hasConcept C185592680 @default.
• W2169733090 hasConcept C189909181 @default.
• W2169733090 hasConcept C20251656 @default.
• W2169733090 hasConcept C2522874641 @default.
• W2169733090 hasConcept C2776625791 @default.
• W2169733090 hasConcept C2776829962 @default.
• W2169733090 hasConcept C2776970464 @default.
• W2169733090 hasConcept C2777284241 @default.
• W2169733090 hasConcept C2777833472 @default.
• W2169733090 hasConcept C2780054949 @default.
• W2169733090 hasConcept C55493867 @default.
• W2169733090 hasConcept C58431802 @default.
• W2169733090 hasConcept C86803240 @default.
• W2169733090 hasConcept C96294017 @default.
• W2169733090 hasConceptScore W2169733090C109110057 @default.
• W2169733090 hasConceptScore W2169733090C122245378 @default.
• W2169733090 hasConceptScore W2169733090C14728290 @default.
• W2169733090 hasConceptScore W2169733090C150757390 @default.
• W2169733090 hasConceptScore W2169733090C153911025 @default.
• W2169733090 hasConceptScore W2169733090C159047783 @default.
• W2169733090 hasConceptScore W2169733090C181199279 @default.
• W2169733090 hasConceptScore W2169733090C185592680 @default.
• W2169733090 hasConceptScore W2169733090C189909181 @default.
• W2169733090 hasConceptScore W2169733090C20251656 @default.
• W2169733090 hasConceptScore W2169733090C2522874641 @default.
• W2169733090 hasConceptScore W2169733090C2776625791 @default.
• W2169733090 hasConceptScore W2169733090C2776829962 @default.
• W2169733090 hasConceptScore W2169733090C2776970464 @default.
• W2169733090 hasConceptScore W2169733090C2777284241 @default.
• W2169733090 hasConceptScore W2169733090C2777833472 @default.
• W2169733090 hasConceptScore W2169733090C2780054949 @default.
• W2169733090 hasConceptScore W2169733090C55493867 @default.
• W2169733090 hasConceptScore W2169733090C58431802 @default.
• W2169733090 hasConceptScore W2169733090C86803240 @default.
• W2169733090 hasConceptScore W2169733090C96294017 @default.
• W2169733090 hasIssue "4" @default.
• W2169733090 hasLocation W21697330901 @default.

• next