FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169735044> ?p ?o ?g. }

• W2169735044 endingPage "2613" @default.
• W2169735044 startingPage "2604" @default.
• W2169735044 abstract "The recently identified CprK branch of the CRP (cyclic AMP receptor protein)-FNR (fumarate and nitrate reduction regulator) family of transcriptional regulators includes proteins that activate the transcription of genes encoding proteins involved in reductive dehalogenation of chlorinated aromatic compounds. Here we report the characterization of the CprK1 protein from Desulfitobacterium hafniense, an anaerobic low-G+C gram-positive bacterium that is capable of reductive dechlorination of 3-chloro-4-hydroxyphenylacetic acid (Cl-OHPA). The gene encoding CprK1 was cloned and functionally overexpressed in Escherichia coli, and the protein was subsequently purified to homogeneity. To investigate the interaction of CprK1 with three of its predicted binding sequences (dehaloboxes), we performed in vitro DNA-binding assays (electrophoretic mobility shift assays) as well as in vivo promoter probe assays. Our results show that CprK1 binds its target dehaloboxes with high affinity (dissociation constant, 90 nM) in the presence of Cl-OHPA and that transcriptional initiation by CprK1 is influenced by deviations in the dehaloboxes from the consensus TTAAT----ATTAA sequence. A mutant CprK1 protein was created by a Val-->Glu substitution at a conserved position in the recognition alpha-helix that gained FNR-type DNA-binding specificity, recognizing the TTGAT----ATCAA sequence (FNR box) instead of the dehaloboxes. CprK1 was subject to oxidative inactivation in vitro, most likely caused by the formation of an intermolecular disulfide bridge between Cys11 and Cys200. The possibility of redox regulation of CprK1 by a thiol-disulfide exchange reaction was investigated by using two Cys-->Ser mutants. Our results indicate that a Cys11-Cys200 disulfide bridge does not appear to play a physiological role in the regulation of CprK1." @default.
• W2169735044 created "2016-06-24" @default.
• W2169735044 creator A5010586842 @default.
• W2169735044 creator A5017248921 @default.
• W2169735044 creator A5019252372 @default.
• W2169735044 creator A5033938158 @default.
• W2169735044 creator A5034147460 @default.
• W2169735044 creator A5068718584 @default.
• W2169735044 creator A5069967673 @default.
• W2169735044 creator A5077803226 @default.
• W2169735044 date "2006-04-01" @default.
• W2169735044 modified "2023-10-12" @default.
• W2169735044 title "Characterization of CprK1, a CRP/FNR-Type Transcriptional Regulator of Halorespiration from <i>Desulfitobacterium hafniense</i>" @default.
• W2169735044 cites W1572249037 @default.
• W2169735044 cites W1589237644 @default.
• W2169735044 cites W1594974221 @default.
• W2169735044 cites W1981092872 @default.
• W2169735044 cites W1981577753 @default.
• W2169735044 cites W1991135410 @default.
• W2169735044 cites W2007013592 @default.
• W2169735044 cites W2007180283 @default.
• W2169735044 cites W2011788811 @default.
• W2169735044 cites W2013617142 @default.
• W2169735044 cites W2016901528 @default.
• W2169735044 cites W2016963768 @default.
• W2169735044 cites W2030566353 @default.
• W2169735044 cites W2032583557 @default.
• W2169735044 cites W2036822952 @default.
• W2169735044 cites W2045203179 @default.
• W2169735044 cites W2060913542 @default.
• W2169735044 cites W2065310225 @default.
• W2169735044 cites W2068315188 @default.
• W2169735044 cites W2075704290 @default.
• W2169735044 cites W2076985939 @default.
• W2169735044 cites W2077100554 @default.
• W2169735044 cites W2078559705 @default.
• W2169735044 cites W2080688660 @default.
• W2169735044 cites W2082783701 @default.
• W2169735044 cites W2082964210 @default.
• W2169735044 cites W2092906794 @default.
• W2169735044 cites W2096586793 @default.
• W2169735044 cites W2098089372 @default.
• W2169735044 cites W2100314338 @default.
• W2169735044 cites W2101415751 @default.
• W2169735044 cites W2118325780 @default.
• W2169735044 cites W2126818765 @default.
• W2169735044 cites W2127699503 @default.
• W2169735044 cites W2127809748 @default.
• W2169735044 cites W2128635872 @default.
• W2169735044 cites W2139171269 @default.
• W2169735044 cites W2156362640 @default.
• W2169735044 cites W2177265400 @default.
• W2169735044 cites W4246590763 @default.
• W2169735044 cites W4293247451 @default.
• W2169735044 cites W98049954 @default.
• W2169735044 doi "https://doi.org/10.1128/jb.188.7.2604-2613.2006" @default.
• W2169735044 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1428393" @default.
• W2169735044 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16547048" @default.
• W2169735044 hasPublicationYear "2006" @default.
• W2169735044 type Work @default.
• W2169735044 sameAs 2169735044 @default.
• W2169735044 citedByCount "39" @default.
• W2169735044 countsByYear W21697350442012 @default.
• W2169735044 countsByYear W21697350442013 @default.
• W2169735044 countsByYear W21697350442014 @default.
• W2169735044 countsByYear W21697350442015 @default.
• W2169735044 countsByYear W21697350442016 @default.
• W2169735044 countsByYear W21697350442017 @default.
• W2169735044 countsByYear W21697350442019 @default.
• W2169735044 countsByYear W21697350442020 @default.
• W2169735044 countsByYear W21697350442021 @default.
• W2169735044 countsByYear W21697350442022 @default.
• W2169735044 countsByYear W21697350442023 @default.
• W2169735044 crossrefType "journal-article" @default.
• W2169735044 hasAuthorship W2169735044A5010586842 @default.
• W2169735044 hasAuthorship W2169735044A5017248921 @default.
• W2169735044 hasAuthorship W2169735044A5019252372 @default.
• W2169735044 hasAuthorship W2169735044A5033938158 @default.
• W2169735044 hasAuthorship W2169735044A5034147460 @default.
• W2169735044 hasAuthorship W2169735044A5068718584 @default.
• W2169735044 hasAuthorship W2169735044A5069967673 @default.
• W2169735044 hasAuthorship W2169735044A5077803226 @default.
• W2169735044 hasBestOaLocation W21697350442 @default.
• W2169735044 hasConcept C104317684 @default.
• W2169735044 hasConcept C107824862 @default.
• W2169735044 hasConcept C143065580 @default.
• W2169735044 hasConcept C153911025 @default.
• W2169735044 hasConcept C167625842 @default.
• W2169735044 hasConcept C183978625 @default.
• W2169735044 hasConcept C547475151 @default.
• W2169735044 hasConcept C552990157 @default.
• W2169735044 hasConcept C55493867 @default.
• W2169735044 hasConcept C86339819 @default.
• W2169735044 hasConcept C86803240 @default.
• W2169735044 hasConcept C94966510 @default.
• W2169735044 hasConceptScore W2169735044C104317684 @default.
• W2169735044 hasConceptScore W2169735044C107824862 @default.
• W2169735044 hasConceptScore W2169735044C143065580 @default.

• next