FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169762912> ?p ?o ?g. }

• W2169762912 endingPage "411" @default.
• W2169762912 startingPage "405" @default.
• W2169762912 abstract "Barrier function and shape changes of endothelial cells (EC) are regulated by phosphorylation/dephosphorylation of key signaling and contractile elements. EC contraction results in intercellular gap formation and loss of the selective vascular barrier to circulating macromolecules. EC dysfunction elicited by thrombin was found to correlate with actin microfilament redistribution. It is known that calcineurin (Cn) is involved in thrombin-induced EC dysfunction because inhibition of Cn potentiates PKC activity and the phosphorylation state of EC myosin light chain is also affected by Cn activity. Immunofluorescent detection of Cn catalytic subunit (CnA) isoforms coexpressed with GFP was visualized on paraformaldehyde (PFA) fixed bovine pulmonary artery endothelial cells (BPAEC). Actin microfilaments were stained with Texas Red-phalloidin. Cytotoxic effects of transfections or treatments and the efficiency of transfections were assessed by flow cytometry. Treatment of BPAEC with Cn inhibitors (cyclosporin A and FK506) hindered recovery of the cells from thrombin-induced EC dysfunction. Inhibition of Cn in the absence of thrombin had no effect on cytoskeletal actin filaments. We detected attenuated thrombin-induced stress fiber formation and changes in cell shape only when cells were transfected with constitutively active CnA and not with various CnA isoforms. Flow cytometry (FCM) analysis has proved that cytotoxic effect of treatments is negligible. We observed that Cn is involved in the recovery from thrombin-induced EC dysfunction. Inhibition of Cn caused prolonged contractile effect, while overexpression of constitutively active CnA resulted in reduced thrombin-induced stress fiber formation." @default.
• W2169762912 created "2016-06-24" @default.
• W2169762912 creator A5005762288 @default.
• W2169762912 creator A5025640951 @default.
• W2169762912 creator A5052491243 @default.
• W2169762912 creator A5060290731 @default.
• W2169762912 creator A5069120220 @default.
• W2169762912 creator A5077174799 @default.
• W2169762912 creator A5087059351 @default.
• W2169762912 date "2009-02-20" @default.
• W2169762912 modified "2023-10-15" @default.
• W2169762912 title "Role of calcineurin in thrombin‐mediated endothelial cell contraction" @default.
• W2169762912 cites W1493538154 @default.
• W2169762912 cites W1621759679 @default.
• W2169762912 cites W1892570697 @default.
• W2169762912 cites W1913765963 @default.
• W2169762912 cites W1974755678 @default.
• W2169762912 cites W1984754431 @default.
• W2169762912 cites W1986394439 @default.
• W2169762912 cites W1991163286 @default.
• W2169762912 cites W2011228288 @default.
• W2169762912 cites W2015106062 @default.
• W2169762912 cites W2017842551 @default.
• W2169762912 cites W2021087951 @default.
• W2169762912 cites W2041512157 @default.
• W2169762912 cites W2052608599 @default.
• W2169762912 cites W2054599718 @default.
• W2169762912 cites W2057602456 @default.
• W2169762912 cites W2060189631 @default.
• W2169762912 cites W2061757955 @default.
• W2169762912 cites W2065631148 @default.
• W2169762912 cites W2070656766 @default.
• W2169762912 cites W2081358368 @default.
• W2169762912 cites W2084147384 @default.
• W2169762912 cites W2089076186 @default.
• W2169762912 cites W2092619158 @default.
• W2169762912 cites W2128273788 @default.
• W2169762912 cites W2134824520 @default.
• W2169762912 cites W2138608519 @default.
• W2169762912 cites W2145315072 @default.
• W2169762912 cites W2152907506 @default.
• W2169762912 cites W2155461712 @default.
• W2169762912 cites W2161049379 @default.
• W2169762912 cites W2172196619 @default.
• W2169762912 cites W2172210768 @default.
• W2169762912 cites W2178456244 @default.
• W2169762912 cites W2179375563 @default.
• W2169762912 cites W2218341073 @default.
• W2169762912 cites W2278791596 @default.
• W2169762912 cites W2343603404 @default.
• W2169762912 cites W2901833066 @default.
• W2169762912 cites W4211142920 @default.
• W2169762912 doi "https://doi.org/10.1002/cyto.a.20707" @default.
• W2169762912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3884896" @default.
• W2169762912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19235203" @default.
• W2169762912 hasPublicationYear "2009" @default.
• W2169762912 type Work @default.
• W2169762912 sameAs 2169762912 @default.
• W2169762912 citedByCount "4" @default.
• W2169762912 countsByYear W21697629122012 @default.
• W2169762912 countsByYear W21697629122019 @default.
• W2169762912 crossrefType "journal-article" @default.
• W2169762912 hasAuthorship W2169762912A5005762288 @default.
• W2169762912 hasAuthorship W2169762912A5025640951 @default.
• W2169762912 hasAuthorship W2169762912A5052491243 @default.
• W2169762912 hasAuthorship W2169762912A5060290731 @default.
• W2169762912 hasAuthorship W2169762912A5069120220 @default.
• W2169762912 hasAuthorship W2169762912A5077174799 @default.
• W2169762912 hasAuthorship W2169762912A5087059351 @default.
• W2169762912 hasBestOaLocation W21697629121 @default.
• W2169762912 hasConcept C11960822 @default.
• W2169762912 hasConcept C125705527 @default.
• W2169762912 hasConcept C126322002 @default.
• W2169762912 hasConcept C128057223 @default.
• W2169762912 hasConcept C142669718 @default.
• W2169762912 hasConcept C1491633281 @default.
• W2169762912 hasConcept C151166631 @default.
• W2169762912 hasConcept C153911025 @default.
• W2169762912 hasConcept C185592680 @default.
• W2169762912 hasConcept C189040839 @default.
• W2169762912 hasConcept C203014093 @default.
• W2169762912 hasConcept C2777292125 @default.
• W2169762912 hasConcept C2781099445 @default.
• W2169762912 hasConcept C2911091166 @default.
• W2169762912 hasConcept C2993400109 @default.
• W2169762912 hasConcept C55493867 @default.
• W2169762912 hasConcept C71924100 @default.
• W2169762912 hasConcept C8035138 @default.
• W2169762912 hasConcept C86803240 @default.
• W2169762912 hasConcept C89560881 @default.
• W2169762912 hasConcept C95444343 @default.
• W2169762912 hasConceptScore W2169762912C11960822 @default.
• W2169762912 hasConceptScore W2169762912C125705527 @default.
• W2169762912 hasConceptScore W2169762912C126322002 @default.
• W2169762912 hasConceptScore W2169762912C128057223 @default.
• W2169762912 hasConceptScore W2169762912C142669718 @default.
• W2169762912 hasConceptScore W2169762912C1491633281 @default.
• W2169762912 hasConceptScore W2169762912C151166631 @default.

• next