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- W2169852161 abstract "Three transposon mutagenesis systems used in Erwinia carotovora subsp. carotovora and E. carotovora subsp. atroseptica are outlined. These include systems based on pJB4JI and bacteriophage λ suicide vectors. The use of one of these systems to isolate a series of Tn5 mutants of E. carotovora subsp. carotovora is described briefly. One cys::Tn5 mutant was characterised in detail and designated 15:29. The cloning of the Tn5 mutation from 15:29 and the construction of a clone carrying the corresponding wild-type gene is described. This is presented as an example of the use of transposons to clone any gene with a selectable phenotype." @default.
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- W2169852161 date "1987-01-01" @default.
- W2169852161 modified "2023-09-27" @default.
- W2169852161 title "The Application of Transposons to the Cloning of Genes from Erwinia Carotovora" @default.
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- W2169852161 doi "https://doi.org/10.1007/978-94-009-3555-6_37" @default.
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