FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2169986504> ?p ?o ?g. }

• W2169986504 endingPage "2049" @default.
• W2169986504 startingPage "2039" @default.
• W2169986504 abstract "ABSTRACT Several type II restriction-modification gene complexes can force their maintenance on their host bacteria by killing cells that have lost them in a process called postsegregational killing or genetic addiction. It is likely to proceed by dilution of the modification enzyme molecule during rounds of cell division following the gene loss, which exposes unmethylated recognition sites on the newly replicated chromosomes to lethal attack by the remaining restriction enzyme molecules. This process is in apparent contrast to the process of the classical types of postsegregational killing systems, in which built-in metabolic instability of the antitoxin allows release of the toxin for lethal action after the gene loss. In the present study, we characterize a mutant form of the EcoRII gene complex that shows stronger capacity in such maintenance. This phenotype is conferred by an L80P amino acid substitution (T239C nucleotide substitution) mutation in the modification enzyme. This mutant enzyme showed decreased DNA methyltransferase activity at a higher temperature in vivo and in vitro than the nonmutated enzyme, although a deletion mutant lacking the N-terminal 83 amino acids did not lose activity at either of the temperatures tested. Under a condition of inhibited protein synthesis, the activity of the L80P mutant was completely lost at a high temperature. In parallel, the L80P mutant protein disappeared more rapidly than the wild-type protein. These results demonstrate that the capability of a restriction-modification system in forcing maintenance on its host can be modulated by a region of its antitoxin, the modification enzyme, as in the classical postsegregational killing systems." @default.
• W2169986504 created "2016-06-24" @default.
• W2169986504 creator A5001805312 @default.
• W2169986504 creator A5022775273 @default.
• W2169986504 creator A5035038653 @default.
• W2169986504 creator A5072068712 @default.
• W2169986504 creator A5087006540 @default.
• W2169986504 date "2008-03-15" @default.
• W2169986504 modified "2023-10-03" @default.
• W2169986504 title "Maintenance Forced by a Restriction-Modification System Can Be Modulated by a Region in Its Modification Enzyme Not Essential for Methyltransferase Activity" @default.
• W2169986504 cites W1559209718 @default.
• W2169986504 cites W1584174944 @default.
• W2169986504 cites W1604915411 @default.
• W2169986504 cites W1803661918 @default.
• W2169986504 cites W1804450209 @default.
• W2169986504 cites W1820567505 @default.
• W2169986504 cites W1847830247 @default.
• W2169986504 cites W1858120728 @default.
• W2169986504 cites W1900133438 @default.
• W2169986504 cites W1904427748 @default.
• W2169986504 cites W1917829332 @default.
• W2169986504 cites W1969460313 @default.
• W2169986504 cites W1971374692 @default.
• W2169986504 cites W1976920382 @default.
• W2169986504 cites W1979509787 @default.
• W2169986504 cites W1979976157 @default.
• W2169986504 cites W1980606854 @default.
• W2169986504 cites W1985347638 @default.
• W2169986504 cites W1993932057 @default.
• W2169986504 cites W2002781072 @default.
• W2169986504 cites W2003803301 @default.
• W2169986504 cites W2005528894 @default.
• W2169986504 cites W2005586161 @default.
• W2169986504 cites W2008528292 @default.
• W2169986504 cites W2012060327 @default.
• W2169986504 cites W2016299826 @default.
• W2169986504 cites W2021197152 @default.
• W2169986504 cites W2022224323 @default.
• W2169986504 cites W2025104601 @default.
• W2169986504 cites W2030094346 @default.
• W2169986504 cites W2034256916 @default.
• W2169986504 cites W2036425633 @default.
• W2169986504 cites W2041486332 @default.
• W2169986504 cites W2043602826 @default.
• W2169986504 cites W2044796250 @default.
• W2169986504 cites W2045921519 @default.
• W2169986504 cites W2055565613 @default.
• W2169986504 cites W2056345505 @default.
• W2169986504 cites W2057044419 @default.
• W2169986504 cites W2057527668 @default.
• W2169986504 cites W2060756371 @default.
• W2169986504 cites W2072086082 @default.
• W2169986504 cites W2072564711 @default.
• W2169986504 cites W2073506102 @default.
• W2169986504 cites W2075518131 @default.
• W2169986504 cites W2076911571 @default.
• W2169986504 cites W2077368990 @default.
• W2169986504 cites W2079726617 @default.
• W2169986504 cites W2090102680 @default.
• W2169986504 cites W2091915227 @default.
• W2169986504 cites W2100878259 @default.
• W2169986504 cites W2101497433 @default.
• W2169986504 cites W2105753010 @default.
• W2169986504 cites W2106150306 @default.
• W2169986504 cites W2108142974 @default.
• W2169986504 cites W2115328763 @default.
• W2169986504 cites W2127643819 @default.
• W2169986504 cites W2128580246 @default.
• W2169986504 cites W2132417094 @default.
• W2169986504 cites W2146346646 @default.
• W2169986504 cites W2155759817 @default.
• W2169986504 cites W2159284171 @default.
• W2169986504 cites W2161247547 @default.
• W2169986504 cites W2170718546 @default.
• W2169986504 cites W2171967661 @default.
• W2169986504 cites W311268221 @default.
• W2169986504 doi "https://doi.org/10.1128/jb.01319-07" @default.
• W2169986504 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2258900" @default.
• W2169986504 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18192396" @default.
• W2169986504 hasPublicationYear "2008" @default.
• W2169986504 type Work @default.
• W2169986504 sameAs 2169986504 @default.
• W2169986504 citedByCount "22" @default.
• W2169986504 countsByYear W21699865042012 @default.
• W2169986504 countsByYear W21699865042013 @default.
• W2169986504 countsByYear W21699865042014 @default.
• W2169986504 countsByYear W21699865042016 @default.
• W2169986504 countsByYear W21699865042017 @default.
• W2169986504 countsByYear W21699865042018 @default.
• W2169986504 countsByYear W21699865042019 @default.
• W2169986504 countsByYear W21699865042020 @default.
• W2169986504 countsByYear W21699865042022 @default.
• W2169986504 countsByYear W21699865042023 @default.
• W2169986504 crossrefType "journal-article" @default.
• W2169986504 hasAuthorship W2169986504A5001805312 @default.
• W2169986504 hasAuthorship W2169986504A5022775273 @default.
• W2169986504 hasAuthorship W2169986504A5035038653 @default.
• W2169986504 hasAuthorship W2169986504A5072068712 @default.

• next