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- W2169992493 abstract "A rapid polymerase chain reaction (PCR) method was developed for detection of low numbers of Campylobacter jejuni. In this method, PCR was run directly from lysed C. jejuni cells and used a short denaturing and annealing time, a rapid transition, and an increased number of cycles to obtain good sensitivity. Only 3 h were required for PCR and 1 h for electrophoresis. Additional time for DNA isolation and DNA hybridization was not needed. This system was positive for C. jejuni but negative for C. coli and C. fetus. the method detected as few as two cells in original liquid ofC. jejuni in pure cultures. an internal probe hybridization test confirmed that the PCR products were from C. jejuni." @default.
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- W2169992493 date "1992-07-01" @default.
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- W2169992493 title "A RAPID PCR METHOD FOR DIRECT DETECTION of LOW NUMBERS of CAMPYLOBACTER JEJUNI" @default.
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- W2169992493 doi "https://doi.org/10.1111/j.1745-4581.1992.tb00074.x" @default.
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