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• W2170069059 abstract "The effects of estradiol (E2) and E2 withdrawal on tissue concentrations of GnRH receptor mRNA were assessed in orchidectomized sheep (wethers). Wethers received E2 (in 20% ethanol-saline [vehicle]) by continuous infusion at rates of 0.31, 1.25, 5, or 20 μg/h (n = 12 animals per group). Control animals received vehicle alone. Anterior pituitary tissue was collected at the end of the 48-h infusion period. Steady-state levels of GnRH receptor mRNA were quantified by solution hybridization. Tissue levels of mRNA were significantly increased above control levels (0.5 ± 0.1 pg/μg RNA) by E2 infusion at 1.25 μg/h (1.4 ± 0.2 pg/μg RNA). Maximal tissue concentrations of GnRH receptor mRNA were induced by E2 delivery at 5 μg/h (2.4 ± 0.3 pg/μg RNA). In a second study, wethers (n = 6 animals per group) received 5 μg E2/h for 24 h. Pituitary tissue was collected at the end of the infusion period or at 12, 24, or 48 h after cessation of E2 delivery. Infusion of E2 induced a 6-fold increase in GnRH receptor mRNA (0.4 ± 0.1 and 2.9 ± 0.6 pg/μg RNA in control and E2-treated groups, respectively). Cessation of E2 delivery resulted in rapid reduction in steady-state levels of GnRH receptor mRNA. Tissue concentrations of receptor mRNA returned to pretreatment levels within 12 h of E2 withdrawal (0.6 ± 0.1 pg/μg RNA). In a third study, the concentration of GnRH receptor mRNA was determined in pituitary tissue collected during preovulatory surge-like secretion induced in E2-infused wethers by episodic delivery of high-amplitude GnRH (1600 ng GnRH per hourly pulse). Estradiol (5 μg/ml in 10% ethanol-saline) was delivered as a continuous infusion. Episodic delivery of GnRH was initiated 24 h after E2 infusion was begun, and concurrent administration of E2 and episodic GnRH was continued to slaughter. Anterior pituitary tissue was collected at 0, 3, 6, 12, or 24 h after beginning circhoral delivery of GnRH (n = 6 wethers per group). As noted above, continuous infusion of E2 for 24 h significantly increased tissue concentrations of GnRH receptor mRNA. However, steady-state levels of GnRH receptor mRNA were returned to pretreatment levels after 3 h of circhoral delivery of GnRH (0.4 ± 0.1 pg/μg RNA). Taken together, these data demonstrate that physiological concentrations of E2 increase steady-state levels of GnRH receptor mRNA in a dose-dependent manner. In addition, continued estrogenic support is required to maintain enhanced tissue concentrations of GnRH receptor mRNA. Finally, high-amplitude GnRH stimulation induces down-regulation of tissue levels of GnRH receptor mRNA. These data suggest that the dynamic changes in tissue concentrations of GnRH receptor mRNA during the periovulatory period may be due to the inductive effects of gonadal steroids from the developing follicle and to the combined suppressive effects of the increased GnRH stimulation and E2 withdrawal that are associated with the gonadotropin surge." @default.
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• W2170069059 title "Concentrations of Gonadotropin-Releasing Hormone (GnRH) Receptor Messenger Ribonucleic Acid in Pituitary Tissue of Orchidectomized Sheep: Effect of Estradiol and GnRH1" @default.
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• W2170069059 doi "https://doi.org/10.1095/biolreprod54.2.407" @default.
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