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• W2170081920 abstract "DNA probes with conjugated minor groove binder (MGB) groups form extremely stable duplexes with single-stranded DNA targets, allowing shorter probes to be used for hybridization based assays. In this paper, sequence specificity of 3'-MGB probes was explored. In comparison with unmodified DNA, MGB probes had higher melting temperature (T(m)) and increased specificity, especially when a mismatch was in the MGB region of the duplex. To exploit these properties, fluorogenic MGB probes were prepared and investigated in the 5'-nuclease PCR assay (real-time PCR assay, TaqMan assay). A 12mer MGB probe had the same T(m)(65 degrees C) as a no-MGB 27mer probe. The fluorogenic MGB probes were more specific for single base mismatches and fluorescence quenching was more efficient, giving increased sensitivity. A/T rich duplexes were stabilized more than G/C rich duplexes, thereby leveling probe T(m)and simplifying design. In summary, MGB probes were more sequence specific than standard DNA probes, especially for single base mismatches at elevated hybridization temperatures." @default.
• W2170081920 created "2016-06-24" @default.
• W2170081920 creator A5049533479 @default.
• W2170081920 date "2000-01-15" @default.
• W2170081920 modified "2023-10-05" @default.
• W2170081920 title "3'-Minor groove binder-DNA probes increase sequence specificity at PCR extension temperatures" @default.
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• W2170081920 doi "https://doi.org/10.1093/nar/28.2.655" @default.
• W2170081920 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/102528" @default.
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