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- W2170126950 abstract "The GP5 gene of porcine reproductive and respiratory syndrome virus(PRRSV) CH-1a strain was amplified from the PRRSV genome by RT-PCR,and cloned into the transfer plasmid pSC11 to produce pSC11-GP5.Recombinant vaccinia virus containing the GP5 gene(rWR-PRRSV-GP5) was generated through homologous recombination between pSC11-GP5 and vaccinia virus WR strain in TK-143 cells,purified by plaque assay and identified by PCR.Expression of the GP5 protein was detected by SDS-PAGE.Antigenicity of the recombinant GP5 protein was confirmed by indirect immunofluorescence assay(IFA).Inoculation to mice with rWR-PRRSV-GP5 induced specific humoral immune responses against PRRSV." @default.
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- W2170126950 date "2009-01-01" @default.
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- W2170126950 title "Expression of PRRSV GP5 in recombinant vaccinia virus." @default.
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