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• W2170170036 abstract "ObjectiveInvestigation of the effects of diallyl sulfide (DAS), a garlic sulfur compound, on joint tissue inflammatory responses induced by monosodium urate (MSU) crystals and interleukin-1β (IL-1β).DesignThe HIG-82 synovial cell line was used to establish the experimental model and DAS regime. Primary cultures of articular chondrocytes and synovial fibroblasts obtained from patients undergoing joint replacement for osteoarthritis were used in experimental studies. Cyclooxygenase (COX) expression following MSU crystal and IL-1β stimulation with/without DAS co-incubation was assessed by reverse transcription-polymerase chain reaction (RT-PCR), western blotting, and immunocytochemistry and nuclear factor-kappa B (NF-κB) activation determined by electrophoretic mobility shift assay. Prostaglandin E2 (PGE2) production was measured by enzyme-linked immunosorbent assay (ELISA). DAS effects on COX gene expression in an MSU crystal-induced acute arthritis in rats were assessed by RT-PCR.ResultsMSU crystals upregulated COX-2 expression in HIG-82 cells and this was inhibited by co-incubation with DAS. DAS inhibited MSU crystal and IL-1β induced elevation of COX-2 expression in primary synovial cells and chondrocytes. Production of PGE2 induced by crystals was suppressed by DAS and celecoxib. MSU crystals had no effect on expression of COX-1 in synovial cells. NF-κB was activated by MSU crystals and this was blocked by DAS. Increased expression of COX-2 in synovium following intraarticular injection of MSU crystals in a rat model was inhibited by co-administration of DAS.ConclusionsDAS prevents IL-1β and MSU crystal induced COX-2 upregulation in synovial cells and chondrocytes and ameliorates crystal induced synovitis potentially through a mechanism involving NF-κB. Anti-inflammatory actions of DAS may be of value in treatment of joint inflammation. Investigation of the effects of diallyl sulfide (DAS), a garlic sulfur compound, on joint tissue inflammatory responses induced by monosodium urate (MSU) crystals and interleukin-1β (IL-1β). The HIG-82 synovial cell line was used to establish the experimental model and DAS regime. Primary cultures of articular chondrocytes and synovial fibroblasts obtained from patients undergoing joint replacement for osteoarthritis were used in experimental studies. Cyclooxygenase (COX) expression following MSU crystal and IL-1β stimulation with/without DAS co-incubation was assessed by reverse transcription-polymerase chain reaction (RT-PCR), western blotting, and immunocytochemistry and nuclear factor-kappa B (NF-κB) activation determined by electrophoretic mobility shift assay. Prostaglandin E2 (PGE2) production was measured by enzyme-linked immunosorbent assay (ELISA). DAS effects on COX gene expression in an MSU crystal-induced acute arthritis in rats were assessed by RT-PCR. MSU crystals upregulated COX-2 expression in HIG-82 cells and this was inhibited by co-incubation with DAS. DAS inhibited MSU crystal and IL-1β induced elevation of COX-2 expression in primary synovial cells and chondrocytes. Production of PGE2 induced by crystals was suppressed by DAS and celecoxib. MSU crystals had no effect on expression of COX-1 in synovial cells. NF-κB was activated by MSU crystals and this was blocked by DAS. Increased expression of COX-2 in synovium following intraarticular injection of MSU crystals in a rat model was inhibited by co-administration of DAS. DAS prevents IL-1β and MSU crystal induced COX-2 upregulation in synovial cells and chondrocytes and ameliorates crystal induced synovitis potentially through a mechanism involving NF-κB. Anti-inflammatory actions of DAS may be of value in treatment of joint inflammation." @default.
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• W2170170036 date "2009-01-01" @default.
• W2170170036 modified "2023-10-14" @default.
• W2170170036 title "Inhibition of cyclooxygenase 2 expression by diallyl sulfide on joint inflammation induced by urate crystal and IL-1β" @default.
• W2170170036 cites W1912102954 @default.
• W2170170036 cites W1922069783 @default.
• W2170170036 cites W1943303117 @default.
• W2170170036 cites W1975650473 @default.
• W2170170036 cites W1978397248 @default.
• W2170170036 cites W1981366899 @default.
• W2170170036 cites W1998447128 @default.
• W2170170036 cites W2000828983 @default.
• W2170170036 cites W2003678590 @default.
• W2170170036 cites W2012573869 @default.
• W2170170036 cites W202378911 @default.
• W2170170036 cites W2031114460 @default.
• W2170170036 cites W2032806251 @default.
• W2170170036 cites W2045002237 @default.
• W2170170036 cites W2047066575 @default.
• W2170170036 cites W2047334057 @default.
• W2170170036 cites W2053866696 @default.
• W2170170036 cites W2063347675 @default.
• W2170170036 cites W2063355095 @default.
• W2170170036 cites W2064358604 @default.
• W2170170036 cites W2066868344 @default.
• W2170170036 cites W2070881538 @default.
• W2170170036 cites W2072339114 @default.
• W2170170036 cites W2073856508 @default.
• W2170170036 cites W2076411929 @default.
• W2170170036 cites W2085834708 @default.
• W2170170036 cites W2094450904 @default.
• W2170170036 cites W2110108812 @default.
• W2170170036 cites W2117169037 @default.
• W2170170036 cites W2120308930 @default.
• W2170170036 cites W2123580807 @default.
• W2170170036 cites W2132848582 @default.
• W2170170036 cites W2133633020 @default.
• W2170170036 cites W2136662809 @default.
• W2170170036 cites W2137981468 @default.
• W2170170036 cites W2140047847 @default.
• W2170170036 cites W2142743808 @default.
• W2170170036 cites W2144031001 @default.
• W2170170036 cites W2144306195 @default.
• W2170170036 cites W2145771743 @default.
• W2170170036 cites W2151534353 @default.
• W2170170036 cites W2151839966 @default.
• W2170170036 cites W2170423033 @default.
• W2170170036 cites W2268058582 @default.
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• W2170170036 doi "https://doi.org/10.1016/j.joca.2008.05.010" @default.
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