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- W2170176202 abstract "Abstract: We have established a DNA typing system for the HLA‐B5 serologically cross‐reactive group (CREG) by means of a two‐step PCR amplification with nested sequence‐specific primers (nPCR‐SSP). The present study provides a low resolution definition of the HLA‐B5 CREG, i.e. identifying polymorphism equivalent to serology. Two different primer combinations allow group‐specific amplification of all HLA‐B5 CREG alleles and other related HLA class I alleles from genomic DNA. The amplified DNA is subjected to a second amplification step using eleven nested primer pairs. This assay permits the detection of the HLA‐B5 CREG specificities B35, B51, B52, B53, and B7801 in all homozygous and heterozygous combinations. Sensitivity and specificity as judged by a blind quality control study investigating a reference panel (n=50) is 100%. Extension of this approach should allow rapid DNA typing of all serologically defined HLA‐B specificities by nPCR‐SSP." @default.
- W2170176202 created "2016-06-24" @default.
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- W2170176202 date "1995-01-01" @default.
- W2170176202 modified "2023-09-30" @default.
- W2170176202 title "Low resolution DNA typing of the HLA-B5 cross-reactive group by nested PCR-SSP" @default.
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- W2170176202 doi "https://doi.org/10.1111/j.1399-0039.1995.tb02411.x" @default.
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