FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170222722> ?p ?o ?g. }

• W2170222722 endingPage "4460" @default.
• W2170222722 startingPage "4455" @default.
• W2170222722 abstract "Tetraploid embryo complementation assay has shown that mouse ES cells alone are capable of supporting embryonic development and adult life of mice. Newly established F(1) hybrid ES cells allow the production of ES cell-derived animals at a high enough efficiency to directly make ES cell-based genetics feasible. Here we report the establishment and characterization of 12 new F(1) hybrid ES cell lines and the use of one of the best (G4) in a gain- and loss-of-function genetic study, where the in vivo phenotypes were assessed directly from ES cell-derived embryos. We found the generation of G4 ES cell-derived animals to be very efficient. Furthermore, even after two consecutive rounds of genetic modifications, the majority of transgenic lines retained the original potential of the parental lines; with 10-40% of chimeras producing ES cell-derived animals/embryos. Using these genetically altered ES cells, this success rate, in most cases, permitted the derivation of a sufficient number of mutants for initial phenotypic analyses only a few weeks after the establishment of the cell lines. Although the experimental design has to take into account a moderate level of uncontrolled damage on ES cell lines, our proof-of-principle experiment provides useful data to assist future designs harnessing the power of this technology to accelerate our understanding of gene function." @default.
• W2170222722 created "2016-06-24" @default.
• W2170222722 creator A5006203859 @default.
• W2170222722 creator A5011105258 @default.
• W2170222722 creator A5016729586 @default.
• W2170222722 creator A5028658969 @default.
• W2170222722 creator A5047463852 @default.
• W2170222722 creator A5067509033 @default.
• W2170222722 creator A5077858999 @default.
• W2170222722 creator A5091646302 @default.
• W2170222722 date "2007-03-13" @default.
• W2170222722 modified "2023-10-16" @default.
• W2170222722 title "Developmental and adult phenotyping directly from mutant embryonic stem cells" @default.
• W2170222722 cites W1516387975 @default.
• W2170222722 cites W1549831272 @default.
• W2170222722 cites W1587959615 @default.
• W2170222722 cites W1601094160 @default.
• W2170222722 cites W1809041433 @default.
• W2170222722 cites W1856781683 @default.
• W2170222722 cites W1966697925 @default.
• W2170222722 cites W1967517562 @default.
• W2170222722 cites W1985564501 @default.
• W2170222722 cites W1986559037 @default.
• W2170222722 cites W1999596958 @default.
• W2170222722 cites W2006844115 @default.
• W2170222722 cites W2008530548 @default.
• W2170222722 cites W2014721239 @default.
• W2170222722 cites W2023630351 @default.
• W2170222722 cites W2024957663 @default.
• W2170222722 cites W2027700219 @default.
• W2170222722 cites W2028352245 @default.
• W2170222722 cites W2038226174 @default.
• W2170222722 cites W2039854579 @default.
• W2170222722 cites W2042055907 @default.
• W2170222722 cites W2043630138 @default.
• W2170222722 cites W2045247659 @default.
• W2170222722 cites W2045899482 @default.
• W2170222722 cites W2048162805 @default.
• W2170222722 cites W2057159461 @default.
• W2170222722 cites W2061104342 @default.
• W2170222722 cites W2066386509 @default.
• W2170222722 cites W2072820298 @default.
• W2170222722 cites W2094212758 @default.
• W2170222722 cites W2100098337 @default.
• W2170222722 cites W2102911949 @default.
• W2170222722 cites W2109197670 @default.
• W2170222722 cites W2116887824 @default.
• W2170222722 cites W2118737272 @default.
• W2170222722 cites W2140612321 @default.
• W2170222722 cites W2141046053 @default.
• W2170222722 cites W2158316748 @default.
• W2170222722 cites W2162254279 @default.
• W2170222722 cites W2162405918 @default.
• W2170222722 cites W2181264335 @default.
• W2170222722 cites W2269271610 @default.
• W2170222722 doi "https://doi.org/10.1073/pnas.0609277104" @default.
• W2170222722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1838622" @default.
• W2170222722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17360545" @default.
• W2170222722 hasPublicationYear "2007" @default.
• W2170222722 type Work @default.
• W2170222722 sameAs 2170222722 @default.
• W2170222722 citedByCount "199" @default.
• W2170222722 countsByYear W21702227222012 @default.
• W2170222722 countsByYear W21702227222013 @default.
• W2170222722 countsByYear W21702227222014 @default.
• W2170222722 countsByYear W21702227222015 @default.
• W2170222722 countsByYear W21702227222016 @default.
• W2170222722 countsByYear W21702227222017 @default.
• W2170222722 countsByYear W21702227222018 @default.
• W2170222722 countsByYear W21702227222019 @default.
• W2170222722 countsByYear W21702227222020 @default.
• W2170222722 countsByYear W21702227222021 @default.
• W2170222722 countsByYear W21702227222022 @default.
• W2170222722 countsByYear W21702227222023 @default.
• W2170222722 crossrefType "journal-article" @default.
• W2170222722 hasAuthorship W2170222722A5006203859 @default.
• W2170222722 hasAuthorship W2170222722A5011105258 @default.
• W2170222722 hasAuthorship W2170222722A5016729586 @default.
• W2170222722 hasAuthorship W2170222722A5028658969 @default.
• W2170222722 hasAuthorship W2170222722A5047463852 @default.
• W2170222722 hasAuthorship W2170222722A5067509033 @default.
• W2170222722 hasAuthorship W2170222722A5077858999 @default.
• W2170222722 hasAuthorship W2170222722A5091646302 @default.
• W2170222722 hasBestOaLocation W21702227221 @default.
• W2170222722 hasConcept C102230213 @default.
• W2170222722 hasConcept C104317684 @default.
• W2170222722 hasConcept C127716648 @default.
• W2170222722 hasConcept C143065580 @default.
• W2170222722 hasConcept C145103041 @default.
• W2170222722 hasConcept C1491633281 @default.
• W2170222722 hasConcept C188082640 @default.
• W2170222722 hasConcept C196843134 @default.
• W2170222722 hasConcept C28328180 @default.
• W2170222722 hasConcept C30278631 @default.
• W2170222722 hasConcept C51456166 @default.
• W2170222722 hasConcept C54009773 @default.
• W2170222722 hasConcept C54355233 @default.
• W2170222722 hasConcept C81885089 @default.

• next