Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170241485> ?p ?o ?g. }
- W2170241485 abstract "Although the ability of estradiol to enhance pituitary sensitivity to GnRH is established, the underlying mechanism(s) remain undefined. Herein, we find that approximately 9,100 bp of 5' flanking region from the ovine GnRH receptor (oGnRHR) gene is devoid of transcriptional activity in gonadotrope-derived cell lines and is not responsive to either estradiol or GnRH. In stark contrast, this same 9,100 bp promoter fragment directed tissue-specific expression of luciferase in multiple lines of transgenic mice. To test for hormonal regulation of the 9,100-bp promoter, ovariectomized transgenic females were treated with a GnRH antiserum alone or in combination with estradiol. Treatment with antiserum alone reduced pituitary expression of luciferase by 80%. Pituitary expression of luciferase in animals receiving both antiserum and estradiol was approximately 50-fold higher than animals receiving antiserum alone. The estradiol response of the -9,100-bp promoter was equally demonstrable in males. In addition, a GnRH analog (D-Ala-6-GnRH) that does not cross-react with the GnRH antiserum restored pituitary expression of luciferase in males passively immunized against GnRH to levels not different from castrate controls. Finally, treatment with both estradiol and D-Ala-6-GnRH increased pituitary expression of luciferase to a level greater than the sum of the individual treatments suggesting synergistic activation of the transgene by these two hormones. Thus, despite the complete absence of transcriptional activity and hormonal responsiveness in vitro, 9,100 bp of proximal promoter from the oGnRHR gene is capable of directing tissue-specific expression and is robustly responsive to both GnRH and estradiol in transgenic mice. To begin to refine the functional boundaries of the critical cis-acting elements, we next constructed transgenic mice harboring a transgene consisting of 2,700 bp of 5' flanking region from the oGnRHR gene fused to luciferase. As with the -9,100 bp promoter, expression of luciferase in the -2,700 lines was primarily confined to the pituitary gland, brain and testes. Furthermore, the passive immunization-hormonal replacement paradigms described above revealed both GnRH and estradiol responsiveness of the -2,700-bp promoter. Thus, 2,700 bp of proximal promoter from the oGnRHR gene is sufficient for tissue-specific expression as well as GnRH and estradiol responsiveness. Given the inability to recapitulate estradiol regulation of GnRHR gene expression in vitro, transgenic mice may represent one of the few viable avenues for ultimately defining the molecular mechanisms underlying estradiol regulation of GnRHR gene expression." @default.
- W2170241485 created "2016-06-24" @default.
- W2170241485 creator A5002211191 @default.
- W2170241485 creator A5021393325 @default.
- W2170241485 creator A5028759921 @default.
- W2170241485 creator A5047349188 @default.
- W2170241485 creator A5060153842 @default.
- W2170241485 creator A5072942159 @default.
- W2170241485 date "2000-03-01" @default.
- W2170241485 modified "2023-09-24" @default.
- W2170241485 title "Responsiveness of the Ovine Gonadotropin-Releasing Hormone Receptor Gene to Estradiol and Gonadotropin-Releasing Hormone Is Not Detectable in Vitro But Is Revealed in Transgenic Mice*" @default.
- W2170241485 cites W1492242941 @default.
- W2170241485 cites W1964752171 @default.
- W2170241485 cites W1977918766 @default.
- W2170241485 cites W1979158254 @default.
- W2170241485 cites W1979699644 @default.
- W2170241485 cites W1986920739 @default.
- W2170241485 cites W1992478303 @default.
- W2170241485 cites W1994061467 @default.
- W2170241485 cites W1995339707 @default.
- W2170241485 cites W1998334964 @default.
- W2170241485 cites W2002526474 @default.
- W2170241485 cites W2006729630 @default.
- W2170241485 cites W2014653843 @default.
- W2170241485 cites W2016065996 @default.
- W2170241485 cites W2017325384 @default.
- W2170241485 cites W2017399638 @default.
- W2170241485 cites W2026110198 @default.
- W2170241485 cites W2029978569 @default.
- W2170241485 cites W2030932806 @default.
- W2170241485 cites W2032728378 @default.
- W2170241485 cites W2038133272 @default.
- W2170241485 cites W2041827938 @default.
- W2170241485 cites W2049757959 @default.
- W2170241485 cites W2055136049 @default.
- W2170241485 cites W2056723048 @default.
- W2170241485 cites W2060990872 @default.
- W2170241485 cites W2065019754 @default.
- W2170241485 cites W2065796880 @default.
- W2170241485 cites W2071920802 @default.
- W2170241485 cites W2072881211 @default.
- W2170241485 cites W2078872477 @default.
- W2170241485 cites W2090977762 @default.
- W2170241485 cites W2091095324 @default.
- W2170241485 cites W2091371511 @default.
- W2170241485 cites W2092279256 @default.
- W2170241485 cites W2092328389 @default.
- W2170241485 cites W2092486725 @default.
- W2170241485 cites W2094796209 @default.
- W2170241485 cites W2108932643 @default.
- W2170241485 cites W2112787832 @default.
- W2170241485 cites W2116567395 @default.
- W2170241485 cites W2127783662 @default.
- W2170241485 cites W2140112004 @default.
- W2170241485 cites W2140440723 @default.
- W2170241485 cites W2163173672 @default.
- W2170241485 cites W230394473 @default.
- W2170241485 cites W238302046 @default.
- W2170241485 doi "https://doi.org/10.1210/endo.141.3.7391" @default.
- W2170241485 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10698176" @default.
- W2170241485 hasPublicationYear "2000" @default.
- W2170241485 type Work @default.
- W2170241485 sameAs 2170241485 @default.
- W2170241485 citedByCount "34" @default.
- W2170241485 countsByYear W21702414852012 @default.
- W2170241485 countsByYear W21702414852013 @default.
- W2170241485 countsByYear W21702414852014 @default.
- W2170241485 countsByYear W21702414852015 @default.
- W2170241485 countsByYear W21702414852016 @default.
- W2170241485 countsByYear W21702414852017 @default.
- W2170241485 countsByYear W21702414852018 @default.
- W2170241485 countsByYear W21702414852019 @default.
- W2170241485 crossrefType "journal-article" @default.
- W2170241485 hasAuthorship W2170241485A5002211191 @default.
- W2170241485 hasAuthorship W2170241485A5021393325 @default.
- W2170241485 hasAuthorship W2170241485A5028759921 @default.
- W2170241485 hasAuthorship W2170241485A5047349188 @default.
- W2170241485 hasAuthorship W2170241485A5060153842 @default.
- W2170241485 hasAuthorship W2170241485A5072942159 @default.
- W2170241485 hasBestOaLocation W21702414851 @default.
- W2170241485 hasConcept C102230213 @default.
- W2170241485 hasConcept C104317684 @default.
- W2170241485 hasConcept C111335760 @default.
- W2170241485 hasConcept C126322002 @default.
- W2170241485 hasConcept C134018914 @default.
- W2170241485 hasConcept C141035611 @default.
- W2170241485 hasConcept C150194340 @default.
- W2170241485 hasConcept C159654299 @default.
- W2170241485 hasConcept C170493617 @default.
- W2170241485 hasConcept C203014093 @default.
- W2170241485 hasConcept C2778575703 @default.
- W2170241485 hasConcept C2778782382 @default.
- W2170241485 hasConcept C2778894405 @default.
- W2170241485 hasConcept C2779029589 @default.
- W2170241485 hasConcept C54009773 @default.
- W2170241485 hasConcept C55493867 @default.
- W2170241485 hasConcept C71315377 @default.
- W2170241485 hasConcept C71924100 @default.
- W2170241485 hasConcept C8264082 @default.
- W2170241485 hasConcept C86803240 @default.