FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170282548> ?p ?o ?g. }

• W2170282548 abstract "Enzyme dynamics has recently been shown to be crucial for structure-function relationship. Among various structure dynamics analysis platforms, HDX (hydrogen deuterium exchange) mass spectrometry stands out as an efficient and high-throughput way to analyze protein dynamics upon ligand binding. Despite the potential, limited research has employed the HDX mass spec platform to probe regional structure dynamics of enzymes. In particular, the technique has never been used for analyzing cell wall degrading enzymes. We hereby used xylanase as a model to explore the potential of HDX mass spectrometry for studying cell wall degrading enzymes. HDX mass spectrometry revealed significant intrinsic dynamics for the xylanase enzyme. Different regions of the enzymes are differentially stabilized in the apo enzyme. The comparison of substrate-binding enzymes revealed that xylohexaose can significantly stabilize the enzyme. Several regions including those near the reaction centres were significantly stabilized during the xylohexaose binding. As compared to xylohexaose, xylan induced relatively less protection in the enzyme, which may be due to the insolubility of the substrate. The structure relevance of the enzyme dynamics was discussed with reference to the three dimensional structure of the enzyme. HDX mass spectrometry revealed strong dynamics-function relevance and such relevance can be explored for the future enzyme improvement. Ligand-binding can lead to the significant stabilization at both regional and global level for enzymes like xylanase. HDX mass spectrometry is a powerful high-throughput platform to identify the key regions protected during the ligand binding and to explore the molecular mechanisms of the enzyme function. The HDX mass spectrometry analysis of cell wall degrading enzymes has provided a novel platform to guide the rational design of enzymes." @default.
• W2170282548 created "2016-06-24" @default.
• W2170282548 creator A5011896955 @default.
• W2170282548 creator A5032786350 @default.
• W2170282548 creator A5052052772 @default.
• W2170282548 creator A5060862551 @default.
• W2170282548 creator A5075854198 @default.
• W2170282548 creator A5082016747 @default.
• W2170282548 date "2010-10-01" @default.
• W2170282548 modified "2023-10-16" @default.
• W2170282548 title "Enzyme structure dynamics of xylanase I from Trichoderma longibrachiatum" @default.
• W2170282548 cites W1980871893 @default.
• W2170282548 cites W1996373816 @default.
• W2170282548 cites W2008609697 @default.
• W2170282548 cites W2008818819 @default.
• W2170282548 cites W2013719504 @default.
• W2170282548 cites W2015188850 @default.
• W2170282548 cites W2015356564 @default.
• W2170282548 cites W2023115763 @default.
• W2170282548 cites W2023912581 @default.
• W2170282548 cites W2041870135 @default.
• W2170282548 cites W2046002103 @default.
• W2170282548 cites W2046790331 @default.
• W2170282548 cites W2048822589 @default.
• W2170282548 cites W2052049981 @default.
• W2170282548 cites W2053839044 @default.
• W2170282548 cites W2055749522 @default.
• W2170282548 cites W2058155379 @default.
• W2170282548 cites W2066961392 @default.
• W2170282548 cites W2071068229 @default.
• W2170282548 cites W2071789190 @default.
• W2170282548 cites W2077757749 @default.
• W2170282548 cites W2077765442 @default.
• W2170282548 cites W2079606633 @default.
• W2170282548 cites W2079959579 @default.
• W2170282548 cites W2083964850 @default.
• W2170282548 cites W2085178886 @default.
• W2170282548 cites W2090341987 @default.
• W2170282548 cites W2091609997 @default.
• W2170282548 cites W2114320763 @default.
• W2170282548 cites W2145419706 @default.
• W2170282548 cites W2155258555 @default.
• W2170282548 cites W2157005876 @default.
• W2170282548 cites W2157271776 @default.
• W2170282548 cites W2161135434 @default.
• W2170282548 doi "https://doi.org/10.1186/1471-2105-11-s6-s12" @default.
• W2170282548 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3026359" @default.
• W2170282548 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20946595" @default.
• W2170282548 hasPublicationYear "2010" @default.
• W2170282548 type Work @default.
• W2170282548 sameAs 2170282548 @default.
• W2170282548 citedByCount "11" @default.
• W2170282548 countsByYear W21702825482012 @default.
• W2170282548 countsByYear W21702825482013 @default.
• W2170282548 countsByYear W21702825482015 @default.
• W2170282548 countsByYear W21702825482017 @default.
• W2170282548 countsByYear W21702825482018 @default.
• W2170282548 countsByYear W21702825482022 @default.
• W2170282548 countsByYear W21702825482023 @default.
• W2170282548 crossrefType "journal-article" @default.
• W2170282548 hasAuthorship W2170282548A5011896955 @default.
• W2170282548 hasAuthorship W2170282548A5032786350 @default.
• W2170282548 hasAuthorship W2170282548A5052052772 @default.
• W2170282548 hasAuthorship W2170282548A5060862551 @default.
• W2170282548 hasAuthorship W2170282548A5075854198 @default.
• W2170282548 hasAuthorship W2170282548A5082016747 @default.
• W2170282548 hasBestOaLocation W21702825481 @default.
• W2170282548 hasConcept C116569031 @default.
• W2170282548 hasConcept C12554922 @default.
• W2170282548 hasConcept C147597530 @default.
• W2170282548 hasConcept C162356407 @default.
• W2170282548 hasConcept C167569220 @default.
• W2170282548 hasConcept C170493617 @default.
• W2170282548 hasConcept C181199279 @default.
• W2170282548 hasConcept C185592680 @default.
• W2170282548 hasConcept C2775859485 @default.
• W2170282548 hasConcept C2775981520 @default.
• W2170282548 hasConcept C43617362 @default.
• W2170282548 hasConcept C55493867 @default.
• W2170282548 hasConcept C59593255 @default.
• W2170282548 hasConcept C86803240 @default.
• W2170282548 hasConceptScore W2170282548C116569031 @default.
• W2170282548 hasConceptScore W2170282548C12554922 @default.
• W2170282548 hasConceptScore W2170282548C147597530 @default.
• W2170282548 hasConceptScore W2170282548C162356407 @default.
• W2170282548 hasConceptScore W2170282548C167569220 @default.
• W2170282548 hasConceptScore W2170282548C170493617 @default.
• W2170282548 hasConceptScore W2170282548C181199279 @default.
• W2170282548 hasConceptScore W2170282548C185592680 @default.
• W2170282548 hasConceptScore W2170282548C2775859485 @default.
• W2170282548 hasConceptScore W2170282548C2775981520 @default.
• W2170282548 hasConceptScore W2170282548C43617362 @default.
• W2170282548 hasConceptScore W2170282548C55493867 @default.
• W2170282548 hasConceptScore W2170282548C59593255 @default.
• W2170282548 hasConceptScore W2170282548C86803240 @default.
• W2170282548 hasIssue "S6" @default.
• W2170282548 hasLocation W21702825481 @default.
• W2170282548 hasLocation W21702825482 @default.
• W2170282548 hasLocation W21702825483 @default.
• W2170282548 hasLocation W21702825484 @default.

• next