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- W2170293208 abstract "Autographa californica nuclear polyhedrosis virus (AcNPV) was used as an expression vector for human beta interferon. By using specially constructed plasmids, the protein-coding sequences for interferon were linked to the AcNPV promoter for the gene encoding for polyhedrin, the major occlusion protein. The interferon gene was inserted at various locations relative to the AcNPV polyhedrin transcriptional and translational signals, and the interferon-polyhedrin hybrid genes were transferred to infectious AcNPV expression vectors. Biologically active interferon was produced, and greater than 95% was secreted from infected insect cells. A maximum of ca. 5 X 10(6) U of interferon activity was produced by 10(6) infected cells. These results demonstrate that AcNPV should be suitable for use as a eucaryotic expression vector for the production of products from cloned genes." @default.
- W2170293208 created "2016-06-24" @default.
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- W2170293208 date "1983-12-01" @default.
- W2170293208 modified "2023-10-03" @default.
- W2170293208 title "Production of human beta interferon in insect cells infected with a baculovirus expression vector." @default.
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- W2170293208 doi "https://doi.org/10.1128/mcb.3.12.2156" @default.
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