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• W2170311423 abstract "Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production." @default.
• W2170311423 created "2016-06-24" @default.
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• W2170311423 date "2015-09-15" @default.
• W2170311423 modified "2023-10-14" @default.
• W2170311423 title "Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose" @default.
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• W2170311423 doi "https://doi.org/10.1186/s13068-015-0324-x" @default.
• W2170311423 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4570510" @default.
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