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• W2170453968 abstract "Presence of E. coli is a useful indicator of fecal contamination in estuarine shellfish. Polymerase chain reaction using primers for uidA were used to detect DNA released from dead E. coli cells and from heatkilled cells in seeded oyster tissue homogenate. Two different methodologies were adopted for DNA extraction from the seeded oyster homogenate: Chelex™ 100 method and a modified cell-lysis method. Results showed PCR amplification of DNA purified by the modified cell-lysis method was able to eliminate detection of cell-free DNA or heat-killed non-viable cells at various concentrations tested as opposed to the Chelex™ 100 method. DNA-PCR detection was positive for uidA using the Chelex™ 100 DNA extraction method on oyster samples with high concentrations (6-100µg) of cell-free DNA or heat-killed cells (1 X 10 6 cells/ml). This suggests that a difference in DNA extraction techniques can eliminate false-positive results by PCR, thus making it a reliable and rapid diagnostic tool to differentiate viable and non-viable cells in shellfish samples. It would greatly enhance the microbiological safety of shellfish and the quality of risk-assessment involved in the detection of viable microbial pathogens in seafood samples." @default.
• W2170453968 created "2016-06-24" @default.
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• W2170453968 date "2002-01-01" @default.
• W2170453968 modified "2023-09-27" @default.
• W2170453968 title "Evaluation of PCR Amplification-based Detection of Heat-killed Escherichia coli and Cell-free DNA in Shellfish" @default.
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