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- W2170629713 abstract "Abstract Tracheas from albino mice were excised and cut into rings approximately 1 mm thick. After pre-incubation overnight in Medium 199 with 5% calf serum, they were placed into fresh medium. Cadmium acetate, copper sulphate or nickel chloride was added separately or in combination, to a final concentration of 10-200 μM. Each metal was also added (10 μM) to serum-free Medium 199. The percentage (0-100) of the inner intact epithelium and the rate (0-4) of the ciliary beat in each ring were assessed before and during a 4-hour incubation period and multiplied to yield a relative activity index (0-400). Cadmium acetate at 10-100 μM depressed the ciliary activity significantly more than 10-100 μM copper sulphate (each level P<0.05). With 200 μM a 50% reduction of the activity index was observed after approximately 35 min. with cadmium acetate and 85 min. with copper sulphate. At 10-200 μM, cadmium acetate was significantly more suppressive than nickel chloride (each level P<0.05). When comparing the inhibitory effects of copper sulphate and nickel chloride a significant difference appeared at 10-100 μM (each level P<0.01), but not at 200 μM (P>0.05). With 200 μM nickel chloride a 50% reduction in the activity index was observed after 90 min. There was a tendency towards reduced toxicity of cadmium acetate when combined with copper sulphate and of copper sulphate when combined with nickel chloride. In serum-free medium metal toxicity was increased." @default.
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- W2170629713 date "2009-03-13" @default.
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- W2170629713 title "Effect of Cadmium Acetate, Copper Sulphate and Nickel Chloride on Organ Cultures of Mouse Trachea" @default.
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- W2170629713 doi "https://doi.org/10.1111/j.1600-0773.1979.tb02305.x" @default.
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