FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170682242> ?p ?o ?g. }

• W2170682242 endingPage "3779" @default.
• W2170682242 startingPage "3767" @default.
• W2170682242 abstract "The varicella-zoster virus (VZV) IE62 protein is the major transcriptional activator. IE62 is capable of associating with DNA both nonspecifically and in a sequence-specific manner via a consensus binding site (5'-ATCGT-3'). However, the function of the consensus site is poorly understood, since IE62 efficiently transactivates promoter elements lacking this sequence. In the work presented here, sequence analysis of the VZV genome revealed the presence of 245 IE62 consensus sites throughout the genome. Some 54 sites were found to be present within putative VZV promoters. Electrophoretic mobility shift assay (EMSA) experiments using an IE62 fragment containing the IE62 DNA-binding domain and duplex oligonucleotides that did or did not contain the IE62 consensus binding sequence yielded K(D) (equilibrium dissociation constant) values in the nanomolar range. Further, the IE62 DNA binding domain was shown to have a 5-fold-increased affinity for its consensus site compared to nonconsensus sequences. The effect of consensus site presence and position on IE62-mediated activation of native VZV and model promoters was examined using site-specific mutagenesis and transfection and superinfection reporter assays. In all promoters examined, the consensus sequence functioned as a distance-dependent repressive element. Protein recruitment assays utilizing the VZV gI promoter indicated that the presence of the consensus site increased the recruitment of IE62 but not Sp1. These data suggest a model where the IE62 consensus site functions to down-modulate IE62 activation, and interaction of IE62 with this sequence may result in loss or decrease of the ability of IE62 to recruit cellular factors needed for full promoter activation." @default.
• W2170682242 created "2016-06-24" @default.
• W2170682242 creator A5041400773 @default.
• W2170682242 creator A5063817051 @default.
• W2170682242 creator A5077600818 @default.
• W2170682242 creator A5084805116 @default.
• W2170682242 date "2010-04-15" @default.
• W2170682242 modified "2023-10-16" @default.
• W2170682242 title "Role of the IE62 Consensus Binding Site in Transactivation by the Varicella-Zoster Virus IE62 Protein" @default.
• W2170682242 cites W1494012774 @default.
• W2170682242 cites W1503919049 @default.
• W2170682242 cites W1520461591 @default.
• W2170682242 cites W1590213056 @default.
• W2170682242 cites W1603100906 @default.
• W2170682242 cites W1603246164 @default.
• W2170682242 cites W1647721669 @default.
• W2170682242 cites W1671632674 @default.
• W2170682242 cites W1763401036 @default.
• W2170682242 cites W1788895899 @default.
• W2170682242 cites W1939294652 @default.
• W2170682242 cites W1954109446 @default.
• W2170682242 cites W1966211185 @default.
• W2170682242 cites W1971209484 @default.
• W2170682242 cites W1972677202 @default.
• W2170682242 cites W1974353442 @default.
• W2170682242 cites W1975911092 @default.
• W2170682242 cites W1983004239 @default.
• W2170682242 cites W1987805321 @default.
• W2170682242 cites W1992578867 @default.
• W2170682242 cites W2002141689 @default.
• W2170682242 cites W2002296591 @default.
• W2170682242 cites W2013138697 @default.
• W2170682242 cites W2021458438 @default.
• W2170682242 cites W2029689060 @default.
• W2170682242 cites W2030823261 @default.
• W2170682242 cites W2030988239 @default.
• W2170682242 cites W2037180441 @default.
• W2170682242 cites W2053168726 @default.
• W2170682242 cites W2060131368 @default.
• W2170682242 cites W2060484288 @default.
• W2170682242 cites W2066276180 @default.
• W2170682242 cites W2073073419 @default.
• W2170682242 cites W2078812139 @default.
• W2170682242 cites W2079314708 @default.
• W2170682242 cites W2079771845 @default.
• W2170682242 cites W2080231602 @default.
• W2170682242 cites W2082080939 @default.
• W2170682242 cites W2084640063 @default.
• W2170682242 cites W2087267626 @default.
• W2170682242 cites W2095963288 @default.
• W2170682242 cites W2096538271 @default.
• W2170682242 cites W2099038638 @default.
• W2170682242 cites W2101202684 @default.
• W2170682242 cites W2124789623 @default.
• W2170682242 cites W2127758278 @default.
• W2170682242 cites W2127996327 @default.
• W2170682242 cites W2129816467 @default.
• W2170682242 cites W2141698737 @default.
• W2170682242 cites W2146123085 @default.
• W2170682242 cites W2154462408 @default.
• W2170682242 cites W2154947167 @default.
• W2170682242 cites W2166369241 @default.
• W2170682242 doi "https://doi.org/10.1128/jvi.02522-09" @default.
• W2170682242 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2849489" @default.
• W2170682242 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20130051" @default.
• W2170682242 hasPublicationYear "2010" @default.
• W2170682242 type Work @default.
• W2170682242 sameAs 2170682242 @default.
• W2170682242 citedByCount "16" @default.
• W2170682242 countsByYear W21706822422012 @default.
• W2170682242 countsByYear W21706822422014 @default.
• W2170682242 countsByYear W21706822422015 @default.
• W2170682242 countsByYear W21706822422016 @default.
• W2170682242 countsByYear W21706822422017 @default.
• W2170682242 countsByYear W21706822422018 @default.
• W2170682242 countsByYear W21706822422019 @default.
• W2170682242 countsByYear W21706822422021 @default.
• W2170682242 countsByYear W21706822422022 @default.
• W2170682242 crossrefType "journal-article" @default.
• W2170682242 hasAuthorship W2170682242A5041400773 @default.
• W2170682242 hasAuthorship W2170682242A5063817051 @default.
• W2170682242 hasAuthorship W2170682242A5077600818 @default.
• W2170682242 hasAuthorship W2170682242A5084805116 @default.
• W2170682242 hasBestOaLocation W21706822422 @default.
• W2170682242 hasConcept C101762097 @default.
• W2170682242 hasConcept C104317684 @default.
• W2170682242 hasConcept C107824862 @default.
• W2170682242 hasConcept C150194340 @default.
• W2170682242 hasConcept C153911025 @default.
• W2170682242 hasConcept C167625842 @default.
• W2170682242 hasConcept C54985914 @default.
• W2170682242 hasConcept C55493867 @default.
• W2170682242 hasConcept C86339819 @default.
• W2170682242 hasConcept C86803240 @default.
• W2170682242 hasConcept C94966510 @default.
• W2170682242 hasConceptScore W2170682242C101762097 @default.
• W2170682242 hasConceptScore W2170682242C104317684 @default.
• W2170682242 hasConceptScore W2170682242C107824862 @default.

• next