Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170698679> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W2170698679 abstract "Publisher Summary This chapter discusses the nicotinamide adenine dinucleotide (NAD + ) kinase from sea urchin eggs. NAD + kinase activity can be measured fluorometrically, by reducing the nicotinamide adenine dinucleotide phosphate (NADP + ) formed to NADPH, with glucose 6-phosphate and glucose 6-phosphate dehydrogenase. NAD + kinase can be stored at 4° as a slurry in 60% saturated (NH 4 ) 2 SO 4 for at least 2 weeks. In solution, between pH 5.5 and 7.0, it is stable at 4° for at least 48 hr. Activity is lost irreversibly below pH 5.5. Dialysis at 4° for 18 h against buffer at pH 8.5 results in the loss of 80% for the activity. This activity can be recovered, by a subsequent 18-hr dialysis, against pH 6.2 buffer. Maximum activity is seen at pH 6.5. Activity decreases sharply below pH 6.0 and above pH 7.0. A divalent cation is required for the activity. With 2 × l0 –3 M NAD + and 10 –3 M adenosine triphosphate (ATP), maximum activity is seen, with 5 × 10 –4 M Mn 2+ . Increasing Mn 2+ concentration results in inhibition. Mg 2+ at 10 –3 M gives 60% of the maximum activity, seen with Mn 2+ , but does not inhibit at higher concentrations. Ca 2+ ,at 5 × 10 –3 M, gives 33% of the maximum activity, seen with Mn 2+ ." @default.
- W2170698679 created "2016-06-24" @default.
- W2170698679 creator A5047247088 @default.
- W2170698679 date "1980-01-01" @default.
- W2170698679 modified "2023-09-24" @default.
- W2170698679 title "[15] NAD+ kinase from sea urchin eggs" @default.
- W2170698679 cites W1556923930 @default.
- W2170698679 cites W2078349619 @default.
- W2170698679 doi "https://doi.org/10.1016/0076-6879(80)66445-3" @default.
- W2170698679 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6246383" @default.
- W2170698679 hasPublicationYear "1980" @default.
- W2170698679 type Work @default.
- W2170698679 sameAs 2170698679 @default.
- W2170698679 citedByCount "6" @default.
- W2170698679 crossrefType "book-chapter" @default.
- W2170698679 hasAuthorship W2170698679A5047247088 @default.
- W2170698679 hasConcept C151730666 @default.
- W2170698679 hasConcept C178790620 @default.
- W2170698679 hasConcept C181199279 @default.
- W2170698679 hasConcept C185592680 @default.
- W2170698679 hasConcept C203014093 @default.
- W2170698679 hasConcept C2776317432 @default.
- W2170698679 hasConcept C2776901170 @default.
- W2170698679 hasConcept C2776908312 @default.
- W2170698679 hasConcept C2777132085 @default.
- W2170698679 hasConcept C2777399203 @default.
- W2170698679 hasConcept C2777989768 @default.
- W2170698679 hasConcept C2779564974 @default.
- W2170698679 hasConcept C2779728014 @default.
- W2170698679 hasConcept C2993802102 @default.
- W2170698679 hasConcept C38485361 @default.
- W2170698679 hasConcept C55493867 @default.
- W2170698679 hasConcept C74884574 @default.
- W2170698679 hasConcept C75520062 @default.
- W2170698679 hasConcept C86803240 @default.
- W2170698679 hasConcept C89560881 @default.
- W2170698679 hasConceptScore W2170698679C151730666 @default.
- W2170698679 hasConceptScore W2170698679C178790620 @default.
- W2170698679 hasConceptScore W2170698679C181199279 @default.
- W2170698679 hasConceptScore W2170698679C185592680 @default.
- W2170698679 hasConceptScore W2170698679C203014093 @default.
- W2170698679 hasConceptScore W2170698679C2776317432 @default.
- W2170698679 hasConceptScore W2170698679C2776901170 @default.
- W2170698679 hasConceptScore W2170698679C2776908312 @default.
- W2170698679 hasConceptScore W2170698679C2777132085 @default.
- W2170698679 hasConceptScore W2170698679C2777399203 @default.
- W2170698679 hasConceptScore W2170698679C2777989768 @default.
- W2170698679 hasConceptScore W2170698679C2779564974 @default.
- W2170698679 hasConceptScore W2170698679C2779728014 @default.
- W2170698679 hasConceptScore W2170698679C2993802102 @default.
- W2170698679 hasConceptScore W2170698679C38485361 @default.
- W2170698679 hasConceptScore W2170698679C55493867 @default.
- W2170698679 hasConceptScore W2170698679C74884574 @default.
- W2170698679 hasConceptScore W2170698679C75520062 @default.
- W2170698679 hasConceptScore W2170698679C86803240 @default.
- W2170698679 hasConceptScore W2170698679C89560881 @default.
- W2170698679 hasLocation W21706986791 @default.
- W2170698679 hasLocation W21706986792 @default.
- W2170698679 hasOpenAccess W2170698679 @default.
- W2170698679 hasPrimaryLocation W21706986791 @default.
- W2170698679 hasRelatedWork W1542883299 @default.
- W2170698679 hasRelatedWork W1585891632 @default.
- W2170698679 hasRelatedWork W2015799885 @default.
- W2170698679 hasRelatedWork W2079340094 @default.
- W2170698679 hasRelatedWork W2093763987 @default.
- W2170698679 hasRelatedWork W2099199907 @default.
- W2170698679 hasRelatedWork W2113816177 @default.
- W2170698679 hasRelatedWork W2170698679 @default.
- W2170698679 hasRelatedWork W2171340535 @default.
- W2170698679 hasRelatedWork W4236954531 @default.
- W2170698679 isParatext "false" @default.
- W2170698679 isRetracted "false" @default.
- W2170698679 magId "2170698679" @default.
- W2170698679 workType "book-chapter" @default.