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• W2170702502 abstract "To identify human intronic sequences associated with 5' splice site recognition, we performed a systematic search for motifs enriched in introns downstream of both constitutive and alternative cassette exons. Significant enrichment was observed for U-rich motifs within 100 nucleotides downstream of 5' splice sites of both classes of exons, with the highest enrichment between positions +6 and +30. Exons adjacent to U-rich intronic motifs contain lower frequencies of exonic splicing enhancers and higher frequencies of exonic splicing silencers, compared with exons not followed by U-rich intronic motifs. These findings motivated us to explore the possibility of a widespread role for U-rich motifs in promoting exon inclusion. Since cytotoxic granule-associated RNA binding protein (TIA1) and TIA1-like 1 (TIAL1; also known as TIAR) were previously shown in vitro to bind to U-rich motifs downstream of 5' splice sites, and to facilitate 5' splice site recognition in vitro and in vivo, we investigated whether these factors function more generally in the regulation of splicing of exons followed by U-rich intronic motifs. Simultaneous knockdown of TIA1 and TIAL1 resulted in increased skipping of 36/41 (88%) of alternatively spliced exons associated with U-rich motifs, but did not affect 32/33 (97%) alternatively spliced exons that are not associated with U-rich motifs. The increase in exon skipping correlated with the proximity of the first U-rich motif and the overall U-richness of the adjacent intronic region. The majority of the alternative splicing events regulated by TIA1/TIAL1 are conserved in mouse, and the corresponding genes are associated with diverse cellular functions. Based on our results, we estimate that approximately 15% of alternative cassette exons are regulated by TIA1/TIAL1 via U-rich intronic elements." @default.
• W2170702502 created "2016-06-24" @default.
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• W2170702502 date "2008-05-02" @default.
• W2170702502 modified "2023-10-02" @default.
• W2170702502 title "A systematic analysis of intronic sequences downstream of 5′ splice sites reveals a widespread role for U-rich motifs and TIA1/TIAL1 proteins in alternative splicing regulation" @default.
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• W2170702502 cites W1971158461 @default.
• W2170702502 cites W1971278266 @default.
• W2170702502 cites W1975406504 @default.
• W2170702502 cites W1975687045 @default.
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• W2170702502 cites W1985991078 @default.
• W2170702502 cites W1986113553 @default.
• W2170702502 cites W1988692621 @default.
• W2170702502 cites W1994823144 @default.
• W2170702502 cites W1994940904 @default.
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• W2170702502 cites W2015809578 @default.
• W2170702502 cites W2019518393 @default.
• W2170702502 cites W2023648355 @default.
• W2170702502 cites W2024267851 @default.
• W2170702502 cites W2026671856 @default.
• W2170702502 cites W2028279880 @default.
• W2170702502 cites W2030409202 @default.
• W2170702502 cites W2031172990 @default.
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• W2170702502 cites W2043435109 @default.
• W2170702502 cites W2045300068 @default.
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• W2170702502 cites W2080290909 @default.
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• W2170702502 cites W2083827172 @default.
• W2170702502 cites W2087815448 @default.
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• W2170702502 doi "https://doi.org/10.1101/gr.073155.107" @default.
• W2170702502 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2493427" @default.
• W2170702502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18456862" @default.
• W2170702502 hasPublicationYear "2008" @default.
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