Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170752642> ?p ?o ?g. }
- W2170752642 endingPage "923" @default.
- W2170752642 startingPage "918" @default.
- W2170752642 abstract "OBJECTIVES: The mechanism by which antiphospholipid antibodies are associated with pregnancy loss and thromboembolism has not been established. We previously showed that annexin-V, a phospholipid-binding protein with potent anticoagulant activity, is present on the apical membranes of the syncytiotrophoblasts that line placental villi and that this protein is reduced, by immunohistochemistry, on placentas of patients with antiphospholipid antibodies. We therefore investigated whether annexin-V in apical membranes of placental villi is quantitatively reduced by antiphospholipid antibody immunoglobulin G. STUDY DESIGN: Placentas were obtained from an index patient with antiphospholipid syndrome with intrauterine growth restriction and from a patient with an uncomplicated pregnancy who were both delivered by cesarean section. Apical villous membranes were isolated and annexin-V levels were measured by enzyme-linked immunosorbent assay. We then studied the effects of antiphospholipid immunoglobulin G on placental villous apical annexin-V in vitro. Antiphospholipid immunoglobulin G was isolated from the sera of five different patients with antiphospholipid antibody syndrome along with five paired control immunoglobulin Gs. Short-term cultures were established from normal placental villi and were exposed to the antibodies, after which isolated apical membranes and culture media were immunoassayed for annexin-V levels. RESULTS: Measurements of apical membrane–associated annexin-V from the antiphospholipid placenta showed significantly less apical membrane–associated annexin-V than did the normal placenta (mean ± SEM: 4.9 ± 0.4 μg/gm villi for antiphospholipid placenta vs 10.2 ± 0.6 μg/gm villi for control, p < 0.001, n = 4). Exposure of placental villous cultures to five different antiphospholipid immunoglobulin Gs for 24 hours resulted in significant reduction of the levels of apical membrane annexin-V (mean ± SEM: 3.9 ± 0.3 μg/gm villi) compared with paired controls (5.1 ± 0.3 μg/gm villi, p = 0.02). Villi incubated with the different antiphospholipid immunoglobulin Gs had significantly less annexin-V in conditioned media (mean ± SEM: 45.1 ± 4.9 ng/gm villi) compared with the paired normal immunoglobulin G control levels (72.6 ± 11.4 ng/gm villi, p = 0.03). CONCLUSIONS: Antiphospholipid immunoglobulin G reduces the levels of syncytiotrophoblast apical membrane–associated annexin-V in placental villi and the release of annexin-V into surrounding media. Reduction of this anticoagulant protein at the maternal-fetal interface may account for the pregnancy loss observed in patients with antiphospholipid syndrome. Short-term culture of placental villi may offer an in vitro model to further study the mechanism of this effect of antiphospholipid antibodies. (Am J Obstet Gynecol 1997;177:918-23.)" @default.
- W2170752642 created "2016-06-24" @default.
- W2170752642 creator A5001956462 @default.
- W2170752642 creator A5005680652 @default.
- W2170752642 creator A5020083084 @default.
- W2170752642 creator A5032273830 @default.
- W2170752642 creator A5064012253 @default.
- W2170752642 creator A5081304304 @default.
- W2170752642 date "1997-10-01" @default.
- W2170752642 modified "2023-09-26" @default.
- W2170752642 title "Antiphospholipid immunoglobulin G antibodies reduce annexin-V levels on syncytiotrophoblast apical membranes and in culture media of placental villi" @default.
- W2170752642 cites W1644153661 @default.
- W2170752642 cites W1966165995 @default.
- W2170752642 cites W1996041636 @default.
- W2170752642 cites W2002611018 @default.
- W2170752642 cites W2004703863 @default.
- W2170752642 cites W2009713220 @default.
- W2170752642 cites W2015315729 @default.
- W2170752642 cites W2031073662 @default.
- W2170752642 cites W2074956393 @default.
- W2170752642 cites W2292475374 @default.
- W2170752642 doi "https://doi.org/10.1016/s0002-9378(97)70294-1" @default.
- W2170752642 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9369845" @default.
- W2170752642 hasPublicationYear "1997" @default.
- W2170752642 type Work @default.
- W2170752642 sameAs 2170752642 @default.
- W2170752642 citedByCount "108" @default.
- W2170752642 countsByYear W21707526422012 @default.
- W2170752642 countsByYear W21707526422013 @default.
- W2170752642 countsByYear W21707526422014 @default.
- W2170752642 countsByYear W21707526422015 @default.
- W2170752642 countsByYear W21707526422016 @default.
- W2170752642 countsByYear W21707526422017 @default.
- W2170752642 countsByYear W21707526422019 @default.
- W2170752642 countsByYear W21707526422020 @default.
- W2170752642 countsByYear W21707526422021 @default.
- W2170752642 countsByYear W21707526422022 @default.
- W2170752642 countsByYear W21707526422023 @default.
- W2170752642 crossrefType "journal-article" @default.
- W2170752642 hasAuthorship W2170752642A5001956462 @default.
- W2170752642 hasAuthorship W2170752642A5005680652 @default.
- W2170752642 hasAuthorship W2170752642A5020083084 @default.
- W2170752642 hasAuthorship W2170752642A5032273830 @default.
- W2170752642 hasAuthorship W2170752642A5064012253 @default.
- W2170752642 hasAuthorship W2170752642A5081304304 @default.
- W2170752642 hasConcept C126322002 @default.
- W2170752642 hasConcept C134018914 @default.
- W2170752642 hasConcept C142724271 @default.
- W2170752642 hasConcept C153911025 @default.
- W2170752642 hasConcept C159654299 @default.
- W2170752642 hasConcept C16685009 @default.
- W2170752642 hasConcept C172680121 @default.
- W2170752642 hasConcept C203014093 @default.
- W2170752642 hasConcept C2776874634 @default.
- W2170752642 hasConcept C2776953305 @default.
- W2170752642 hasConcept C2779234561 @default.
- W2170752642 hasConcept C2779315647 @default.
- W2170752642 hasConcept C2780670749 @default.
- W2170752642 hasConcept C2780898057 @default.
- W2170752642 hasConcept C2780899479 @default.
- W2170752642 hasConcept C3018436504 @default.
- W2170752642 hasConcept C529295009 @default.
- W2170752642 hasConcept C54355233 @default.
- W2170752642 hasConcept C553184892 @default.
- W2170752642 hasConcept C71924100 @default.
- W2170752642 hasConcept C86803240 @default.
- W2170752642 hasConcept C88634738 @default.
- W2170752642 hasConceptScore W2170752642C126322002 @default.
- W2170752642 hasConceptScore W2170752642C134018914 @default.
- W2170752642 hasConceptScore W2170752642C142724271 @default.
- W2170752642 hasConceptScore W2170752642C153911025 @default.
- W2170752642 hasConceptScore W2170752642C159654299 @default.
- W2170752642 hasConceptScore W2170752642C16685009 @default.
- W2170752642 hasConceptScore W2170752642C172680121 @default.
- W2170752642 hasConceptScore W2170752642C203014093 @default.
- W2170752642 hasConceptScore W2170752642C2776874634 @default.
- W2170752642 hasConceptScore W2170752642C2776953305 @default.
- W2170752642 hasConceptScore W2170752642C2779234561 @default.
- W2170752642 hasConceptScore W2170752642C2779315647 @default.
- W2170752642 hasConceptScore W2170752642C2780670749 @default.
- W2170752642 hasConceptScore W2170752642C2780898057 @default.
- W2170752642 hasConceptScore W2170752642C2780899479 @default.
- W2170752642 hasConceptScore W2170752642C3018436504 @default.
- W2170752642 hasConceptScore W2170752642C529295009 @default.
- W2170752642 hasConceptScore W2170752642C54355233 @default.
- W2170752642 hasConceptScore W2170752642C553184892 @default.
- W2170752642 hasConceptScore W2170752642C71924100 @default.
- W2170752642 hasConceptScore W2170752642C86803240 @default.
- W2170752642 hasConceptScore W2170752642C88634738 @default.
- W2170752642 hasIssue "4" @default.
- W2170752642 hasLocation W21707526421 @default.
- W2170752642 hasLocation W21707526422 @default.
- W2170752642 hasOpenAccess W2170752642 @default.
- W2170752642 hasPrimaryLocation W21707526421 @default.
- W2170752642 hasRelatedWork W1866641948 @default.
- W2170752642 hasRelatedWork W2071754429 @default.
- W2170752642 hasRelatedWork W2073891285 @default.
- W2170752642 hasRelatedWork W2075318388 @default.