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• W2170800254 abstract "A gene bank of random fragments of chromosomal DNA from Treponema denticola ATCC 33520 was constructed in the bacteriophage vector λL47.1. The gene bank was plated on Escherichia coli C600 and screened for the presence of plaques in which enzyme activity was expressed, using overlays of fluorogenic synthetic substrates and a two-step procedure in which immunological screening was followed by enzyme assays of immunopositive lysates. Recombinants expressing trypsin-like, chymotrypsin-like and proline iminopeptidase activities were found. The gene for the trypsin-like activity was subcloned into plasmid pJDC9 and maintained in E. coli." @default.
• W2170800254 created "2016-06-24" @default.
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• W2170800254 creator A5056980040 @default.
• W2170800254 creator A5091534447 @default.
• W2170800254 date "1991-10-01" @default.
• W2170800254 modified "2023-10-18" @default.
• W2170800254 title "Cloning and expression of protease genes from Treponema denticola in Escherichia coli" @default.
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• W2170800254 doi "https://doi.org/10.1111/j.1399-302x.1991.tb00490.x" @default.
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