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- W2170808152 abstract "ABSTRACT The processing and methylation of precursor rRNA is mediated by the box C/D small nucleolar RNAs (snoRNAs). These snoRNAs differ from most cellular RNAs in that they are not exported to the cytoplasm. Instead, these RNAs are actively retained in the nucleus where they assemble with proteins into mature small nucleolar ribonucleoprotein particles and are targeted to their intranuclear site of action, the nucleolus. In this study, we have identified the cis -acting sequences responsible for the nuclear retention of U3 box C/D snoRNA by analyzing the nucleocytoplasmic distributions of an extensive panel of U3 RNA variants after injection of the RNAs into Xenopus oocyte nuclei. Our data indicate the importance of two conserved sequence motifs in retaining U3 RNA in the nucleus. The first motif is comprised of the conserved box C′ and box D sequences that characterize the box C/D family. The second motif contains conserved box sequences B and C. Either motif is sufficient for nuclear retention, but disruption of both motifs leads to mislocalization of the RNAs to the cytoplasm. Variant RNAs that are not retained also lack 5′ cap hypermethylation and fail to associate with fibrillarin. Furthermore, our results indicate that nuclear retention of U3 RNA does not simply reflect its nucleolar localization. A fragment of U3 containing the box B/C motif is not localized to nucleoli but retained in coiled bodies. Thus, nuclear retention and nucleolar localization are distinct processes with differing sequence requirements." @default.
- W2170808152 created "2016-06-24" @default.
- W2170808152 creator A5002716376 @default.
- W2170808152 creator A5020042552 @default.
- W2170808152 creator A5036392835 @default.
- W2170808152 creator A5064359286 @default.
- W2170808152 date "1999-12-01" @default.
- W2170808152 modified "2023-10-15" @default.
- W2170808152 title "Nuclear Retention Elements of U3 Small Nucleolar RNA" @default.
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- W2170808152 doi "https://doi.org/10.1128/mcb.19.12.8412" @default.
- W2170808152 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/84939" @default.
- W2170808152 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10567566" @default.
- W2170808152 hasPublicationYear "1999" @default.
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