FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2170816478> ?p ?o ?g. }

• W2170816478 endingPage "1568" @default.
• W2170816478 startingPage "1561" @default.
• W2170816478 abstract "Presentation of antigenic peptides by MHC II molecules is required to initiate CD4 Th cell responses. Some peptides, however, because of low affinity for MHC II, are not efficiently presented. A segment of the MHC II chaperon molecule, invariant chain (Ii), is known to bind early in biosynthesis with low affinity to the peptide binding groove. Here we have exploited the properties of Ii to manipulate the MHC II-loading pathway and to present low-affinity sequences. We used a deletion mutant of Ii where the promiscuous binding site to MHC II, which is adjacent to the groove binding segment, was deleted. A recombinant Ii (rIi) chimera, derived from this construct, was made in which the class II binding segment was exchanged for wild-type or single amino acid substitution variants of an HLA-DR1-restricted sequence from influenza matrix protein (MAT), which leads to MHC II allotype-specific binding. This rIi was expressed in antigen-presenting cells (APC) and introduced the MAT sequence into the MHC II-processing pathway. As expected, rIiMAT elicited antigen-specific, DR1-restricted T cell cytokine production and proliferation. Significantly, rIiMAT, that binds the HLA-DR4 allele with low affinity, elicited DR4-restricted IL-2 production but not proliferation. In contrast, exogenously provided MAT peptide failed to elicit any responses from DR4-restricted T cells. Compatible results were obtained with a single amino acid substitution variant (MATT), which binds with high affinity to DR4 but low affinity to DR1. We conclude that loading of MHC II with antigenic peptides from endogenously synthesized rIi chimeras allows presentation of low-affinity sequences that cannot be presented if provided exogenously as peptides. Ii fusion proteins containing low-affinity antigenic sequences might be useful for vaccination with tumor antigens to overcome deficiencies in antigen presentation." @default.
• W2170816478 created "2016-06-24" @default.
• W2170816478 creator A5004847558 @default.
• W2170816478 creator A5010998605 @default.
• W2170816478 creator A5041058094 @default.
• W2170816478 creator A5044813060 @default.
• W2170816478 creator A5048977579 @default.
• W2170816478 date "2000-11-01" @default.
• W2170816478 modified "2023-09-29" @default.
• W2170816478 title "Invariant chains with the class II binding site replaced by a sequence from influenza virus matrix protein constrain low-affinity sequences to MHC II presentation" @default.
• W2170816478 cites W1494541966 @default.
• W2170816478 cites W1584454554 @default.
• W2170816478 cites W1588864421 @default.
• W2170816478 cites W1590813353 @default.
• W2170816478 cites W1598424666 @default.
• W2170816478 cites W1734730736 @default.
• W2170816478 cites W1918119607 @default.
• W2170816478 cites W1937758884 @default.
• W2170816478 cites W1965546174 @default.
• W2170816478 cites W1966023886 @default.
• W2170816478 cites W1970654655 @default.
• W2170816478 cites W1980008292 @default.
• W2170816478 cites W1982319833 @default.
• W2170816478 cites W1992359551 @default.
• W2170816478 cites W1995482121 @default.
• W2170816478 cites W2001737867 @default.
• W2170816478 cites W2018518355 @default.
• W2170816478 cites W2021187987 @default.
• W2170816478 cites W2027196213 @default.
• W2170816478 cites W2035960239 @default.
• W2170816478 cites W2046202438 @default.
• W2170816478 cites W2052885171 @default.
• W2170816478 cites W2066844508 @default.
• W2170816478 cites W2086404169 @default.
• W2170816478 cites W2088167128 @default.
• W2170816478 cites W2095352046 @default.
• W2170816478 cites W2095843724 @default.
• W2170816478 cites W2104901056 @default.
• W2170816478 cites W2107857115 @default.
• W2170816478 cites W2145100331 @default.
• W2170816478 cites W2147047072 @default.
• W2170816478 cites W2153284406 @default.
• W2170816478 cites W2157352972 @default.
• W2170816478 cites W2164887882 @default.
• W2170816478 cites W2171230527 @default.
• W2170816478 cites W2322011954 @default.
• W2170816478 cites W4239338792 @default.
• W2170816478 cites W4381156449 @default.
• W2170816478 doi "https://doi.org/10.1093/intimm/12.11.1561" @default.
• W2170816478 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11058576" @default.
• W2170816478 hasPublicationYear "2000" @default.
• W2170816478 type Work @default.
• W2170816478 sameAs 2170816478 @default.
• W2170816478 citedByCount "10" @default.
• W2170816478 countsByYear W21708164782013 @default.
• W2170816478 countsByYear W21708164782019 @default.
• W2170816478 countsByYear W21708164782020 @default.
• W2170816478 crossrefType "journal-article" @default.
• W2170816478 hasAuthorship W2170816478A5004847558 @default.
• W2170816478 hasAuthorship W2170816478A5010998605 @default.
• W2170816478 hasAuthorship W2170816478A5041058094 @default.
• W2170816478 hasAuthorship W2170816478A5044813060 @default.
• W2170816478 hasAuthorship W2170816478A5048977579 @default.
• W2170816478 hasBestOaLocation W21708164781 @default.
• W2170816478 hasConcept C119997001 @default.
• W2170816478 hasConcept C147483822 @default.
• W2170816478 hasConcept C153911025 @default.
• W2170816478 hasConcept C154317977 @default.
• W2170816478 hasConcept C170627219 @default.
• W2170816478 hasConcept C185592680 @default.
• W2170816478 hasConcept C197695756 @default.
• W2170816478 hasConcept C202751555 @default.
• W2170816478 hasConcept C207936829 @default.
• W2170816478 hasConcept C2778814158 @default.
• W2170816478 hasConcept C54355233 @default.
• W2170816478 hasConcept C55493867 @default.
• W2170816478 hasConcept C83464605 @default.
• W2170816478 hasConcept C86803240 @default.
• W2170816478 hasConceptScore W2170816478C119997001 @default.
• W2170816478 hasConceptScore W2170816478C147483822 @default.
• W2170816478 hasConceptScore W2170816478C153911025 @default.
• W2170816478 hasConceptScore W2170816478C154317977 @default.
• W2170816478 hasConceptScore W2170816478C170627219 @default.
• W2170816478 hasConceptScore W2170816478C185592680 @default.
• W2170816478 hasConceptScore W2170816478C197695756 @default.
• W2170816478 hasConceptScore W2170816478C202751555 @default.
• W2170816478 hasConceptScore W2170816478C207936829 @default.
• W2170816478 hasConceptScore W2170816478C2778814158 @default.
• W2170816478 hasConceptScore W2170816478C54355233 @default.
• W2170816478 hasConceptScore W2170816478C55493867 @default.
• W2170816478 hasConceptScore W2170816478C83464605 @default.
• W2170816478 hasConceptScore W2170816478C86803240 @default.
• W2170816478 hasIssue "11" @default.
• W2170816478 hasLocation W21708164781 @default.
• W2170816478 hasLocation W21708164782 @default.
• W2170816478 hasOpenAccess W2170816478 @default.
• W2170816478 hasPrimaryLocation W21708164781 @default.
• W2170816478 hasRelatedWork W2023194132 @default.

• next