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• W2170875797 abstract "The transient existence of small RNAs free of binding to the RNA chaperone Hfq is part of the normal dynamic lifecycle of a sRNA. Small RNAs are extremely labile when not associated with Hfq, but the mechanism by which Hfq stabilizes sRNAs has been elusive. In this work we have found that polynucleotide phosphorylase (PNPase) is the major factor involved in the rapid degradation of small RNAs, especially those that are free of binding to Hfq. The levels of MicA, GlmY, RyhB, and SgrS RNAs are drastically increased upon PNPase inactivation in Hfq − cells. In the absence of Hfq, all sRNAs are slightly shorter than their full-length species as result of 3′-end trimming. We show that the turnover of Hfq-free small RNAs is growth-phase regulated, and that PNPase activity is particularly important in stationary phase. Indeed, PNPase makes a greater contribution than RNase E, which is commonly believed to be the main enzyme in the decay of small RNAs. Lack of poly(A) polymerase I (PAP I) is also found to affect the rapid degradation of Hfq-free small RNAs, although to a lesser extent. Our data also suggest that when the sRNA is not associated with Hfq, the degradation occurs mainly in a target-independent pathway in which RNase III has a reduced impact. This work demonstrated that small RNAs free of Hfq binding are preferably degraded by PNPase. Overall, our data highlight the impact of 3′-exonucleolytic RNA decay pathways and re-evaluates the degradation mechanisms of Hfq-free small RNAs." @default.
• W2170875797 created "2016-06-24" @default.
• W2170875797 creator A5012604599 @default.
• W2170875797 creator A5039556725 @default.
• W2170875797 creator A5056800453 @default.
• W2170875797 creator A5084693518 @default.
• W2170875797 date "2012-02-21" @default.
• W2170875797 modified "2023-10-15" @default.
• W2170875797 title "The crucial role of PNPase in the degradation of small RNAs that are not associated with Hfq" @default.
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• W2170875797 doi "https://doi.org/10.1261/rna.029413.111" @default.
• W2170875797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3312570" @default.
• W2170875797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22355164" @default.
• W2170875797 hasPublicationYear "2012" @default.
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