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• W2170985871 abstract "Escherichia coli DNA-directed RNA polymerase is shown to contain a novel phosphorolytic error correction activity which removes erroneous nucleotides, as rNDPs, from the 3′-end of the growing transcript. The activity we describe is biochemically similar to poly-nucleotide phosphorylase (PNP), yet in contrast to PNP is activated by Mn2+. We demonstrate that the activity, which is mediated by Pi, is dependent on the presence of an incorrectly incorporated nucleotide at the leading 3′-end of the transcript. The correction activity we describe exhibits a 4 × 104-fold preference for the excision of incorrect nucleotides from the transcript. These findings suggest the possibility that RNA phosphorolysis may play a critical role in the process of transcriptionai proofreading." @default.
• W2170985871 created "2016-06-24" @default.
• W2170985871 creator A5046955485 @default.
• W2170985871 creator A5055056347 @default.
• W2170985871 date "1994-04-01" @default.
• W2170985871 modified "2023-09-26" @default.
• W2170985871 title "Phosphorolytic error correction during transcription" @default.
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• W2170985871 doi "https://doi.org/10.1111/j.1365-2958.1994.tb01001.x" @default.
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