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• W2170996911 abstract "Anoxygenic photosynthetic electron transport from sulphide, culminating in either H2 evolution or CO2 photoassimilation, was shown to include the segment from plastoquinone to ferredoxin in the cyanobacterium Oscillatoria limnetica. Both sulphide-dependent H2 evolution and CO2 photoassimilation were inhibited by plastoquinone analogues. In the former reaction, the block was bypassed by reduced N,N,N′,N′ -tetramethyl-p-phenylenediamine (TMPD). The link between this segment of electron transport and the hydrogenase enzyme was shown to limit the rate of sulphide-dependent H2 evolution. The rate of flow of electrons through this pathway was lower than would be expected either from the amounts of available enzyme, as measured by the in vitro oxidation of reduced methyl viologen, or from rates of electron transport to CO2 photoassimilation. When the strong reductant sodium dithionite was added to intact cells, the resulting low redox potential significantly improved photosynthetic sulphide-dependent H2 evolution. Hydrogenase activity in cell free extracts was similarly affected by dithionite. It is suggested that ambient redox potential controls electron flow through the hydrogenase, so that surplus reducing power is removed via H2 evolution." @default.
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• W2170996911 date "1983-10-01" @default.
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• W2170996911 title "Low Redox Potential Promotes Sulphide- and Light-dependent Hydrogen Evolution in Oscillatoria limnetica" @default.
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• W2170996911 doi "https://doi.org/10.1099/00221287-129-10-3091" @default.
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