FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171036717> ?p ?o ?g. }

• W2171036717 endingPage "2541" @default.
• W2171036717 startingPage "2541" @default.
• W2171036717 abstract "Cell-free protein synthesis (CFPS) is an alternative approach to cell-based recombinant protein production. It involves in vitro transcription and translation in a cell-free medium. In this work, we implemented CFPS in a plastic array device. Each unit in the array consisted of an inner well and an outer well. Two synthesis steps, gene transcription and protein translation, took place in the inner well, in which a cell-free medium was used to provide ribosomes and additional components necessary for protein synthesis. The outer well was concentric to the inner well and it functioned as a nutrient reservoir. A nanoporous membrane was sandwiched between the inner and outer wells for retaining the synthesized proteins and removing the reaction byproducts. A microfluidic channel was employed to connect these two wells for supplying fresh nutrients for longer reaction time and higher expression yield. Synthesis of luciferase was shown to last 8 times longer and yield 10 times more proteins than in a conventional container. The device also enables more than 2 orders of magnitude reduction in reagent consumption compared to a bench-top instrument. The effects of the membrane pore size and microfluidic channel on the protein production yield were also studied. The array device has potential to become a platform for parallel protein expression for proteomics applications, matching high-throughput gene discovery." @default.
• W2171036717 created "2016-06-24" @default.
• W2171036717 creator A5014869521 @default.
• W2171036717 creator A5020886212 @default.
• W2171036717 creator A5030095106 @default.
• W2171036717 creator A5033803675 @default.
• W2171036717 date "2010-01-01" @default.
• W2171036717 modified "2023-10-14" @default.
• W2171036717 title "Protein synthesis in a device with nanoporous membranes and microchannels" @default.
• W2171036717 cites W1491684093 @default.
• W2171036717 cites W1587481053 @default.
• W2171036717 cites W1963961954 @default.
• W2171036717 cites W1970659557 @default.
• W2171036717 cites W1979915494 @default.
• W2171036717 cites W1982214572 @default.
• W2171036717 cites W1984168659 @default.
• W2171036717 cites W1995151269 @default.
• W2171036717 cites W1995524985 @default.
• W2171036717 cites W1999922729 @default.
• W2171036717 cites W2001516479 @default.
• W2171036717 cites W2004674071 @default.
• W2171036717 cites W2018129784 @default.
• W2171036717 cites W2027912527 @default.
• W2171036717 cites W2031819849 @default.
• W2171036717 cites W2034731045 @default.
• W2171036717 cites W2062876104 @default.
• W2171036717 cites W2070412459 @default.
• W2171036717 cites W2072145399 @default.
• W2171036717 cites W2075002407 @default.
• W2171036717 cites W2080259922 @default.
• W2171036717 cites W2081682162 @default.
• W2171036717 cites W2081710292 @default.
• W2171036717 cites W2082783652 @default.
• W2171036717 cites W2084292270 @default.
• W2171036717 cites W2090560901 @default.
• W2171036717 cites W2091404180 @default.
• W2171036717 cites W2092975262 @default.
• W2171036717 cites W2112758080 @default.
• W2171036717 cites W2121127710 @default.
• W2171036717 cites W2146561267 @default.
• W2171036717 doi "https://doi.org/10.1039/c005233g" @default.
• W2171036717 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20730191" @default.
• W2171036717 hasPublicationYear "2010" @default.
• W2171036717 type Work @default.
• W2171036717 sameAs 2171036717 @default.
• W2171036717 citedByCount "8" @default.
• W2171036717 countsByYear W21710367172012 @default.
• W2171036717 countsByYear W21710367172013 @default.
• W2171036717 countsByYear W21710367172014 @default.
• W2171036717 countsByYear W21710367172015 @default.
• W2171036717 countsByYear W21710367172021 @default.
• W2171036717 crossrefType "journal-article" @default.
• W2171036717 hasAuthorship W2171036717A5014869521 @default.
• W2171036717 hasAuthorship W2171036717A5020886212 @default.
• W2171036717 hasAuthorship W2171036717A5030095106 @default.
• W2171036717 hasAuthorship W2171036717A5033803675 @default.
• W2171036717 hasConcept C104317684 @default.
• W2171036717 hasConcept C105580179 @default.
• W2171036717 hasConcept C12554922 @default.
• W2171036717 hasConcept C134121241 @default.
• W2171036717 hasConcept C144647389 @default.
• W2171036717 hasConcept C149364088 @default.
• W2171036717 hasConcept C171250308 @default.
• W2171036717 hasConcept C185592680 @default.
• W2171036717 hasConcept C191897082 @default.
• W2171036717 hasConcept C191908910 @default.
• W2171036717 hasConcept C192562407 @default.
• W2171036717 hasConcept C2778665413 @default.
• W2171036717 hasConcept C3675279 @default.
• W2171036717 hasConcept C41625074 @default.
• W2171036717 hasConcept C48940184 @default.
• W2171036717 hasConcept C55493867 @default.
• W2171036717 hasConcept C60644358 @default.
• W2171036717 hasConcept C8673954 @default.
• W2171036717 hasConcept C86803240 @default.
• W2171036717 hasConceptScore W2171036717C104317684 @default.
• W2171036717 hasConceptScore W2171036717C105580179 @default.
• W2171036717 hasConceptScore W2171036717C12554922 @default.
• W2171036717 hasConceptScore W2171036717C134121241 @default.
• W2171036717 hasConceptScore W2171036717C144647389 @default.
• W2171036717 hasConceptScore W2171036717C149364088 @default.
• W2171036717 hasConceptScore W2171036717C171250308 @default.
• W2171036717 hasConceptScore W2171036717C185592680 @default.
• W2171036717 hasConceptScore W2171036717C191897082 @default.
• W2171036717 hasConceptScore W2171036717C191908910 @default.
• W2171036717 hasConceptScore W2171036717C192562407 @default.
• W2171036717 hasConceptScore W2171036717C2778665413 @default.
• W2171036717 hasConceptScore W2171036717C3675279 @default.
• W2171036717 hasConceptScore W2171036717C41625074 @default.
• W2171036717 hasConceptScore W2171036717C48940184 @default.
• W2171036717 hasConceptScore W2171036717C55493867 @default.
• W2171036717 hasConceptScore W2171036717C60644358 @default.
• W2171036717 hasConceptScore W2171036717C8673954 @default.
• W2171036717 hasConceptScore W2171036717C86803240 @default.
• W2171036717 hasIssue "19" @default.
• W2171036717 hasLocation W21710367171 @default.
• W2171036717 hasLocation W21710367172 @default.
• W2171036717 hasOpenAccess W2171036717 @default.

• next