FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171067064> ?p ?o ?g. }

• W2171067064 endingPage "500" @default.
• W2171067064 startingPage "491" @default.
• W2171067064 abstract "The presence of multiple cell types in bone marrow stromal populations complicates interpretation of cytokine and hormone effects on the osteoprogenitors present, indicating a need for a method for purification of the osteoprogenitor population. Flow cytometric sorting of 7 day primary rat bone marrow stromal cell cultures was performed on the basis of alkaline phosphatase (AP) expression with an antibody against AP (RBM 211.13). The resultant AP(high), AP(low), or control cells were plated to determine osteoprogenitor, macrophage, and adipocyte distribution and frequency. Approximately 50% of osteoprogenitor/bone nodule-forming cells were lost during processing/sorting when compared with unsorted controls. Nevertheless, within the AP(high) fraction, the numbers of AP-positive colonies and osteoprogenitors (bone nodules) were significantly enriched compared with the unfractionated control; the increase in osteoprogenitor frequency ranged from approximately 2 to 100-fold. There were few assayable osteoprogenitors in either the AP(high) or AP(low) fractions in the absence of dexamethasone (dex), suggesting that RBM stroma contains largely dex-dependent osteoprogenitor populations, and that dex may regulate osteoprogenitors subsequent to the upregulation of AP. Osteoprogenitor/bone nodule numbers in either the AP(high) or AP(low) fraction did not follow a linear relationship with decreasing plating density. The AP(high) fraction of cells was depleted for adipocyte and macrophage colonies. In contrast, within the AP(low) fraction of cells, adipocyte and macrophage colonies were consistently enriched. We conclude that flow-cytometric sorting of RBM stromal populations according to high or low AP expression is an effective technique for enrichment of AP-positive colonies and osteoprogenitors/bone nodule-forming cells." @default.
• W2171067064 created "2016-06-24" @default.
• W2171067064 creator A5031900884 @default.
• W2171067064 creator A5056773555 @default.
• W2171067064 date "1997-12-01" @default.
• W2171067064 modified "2023-10-15" @default.
• W2171067064 title "Cell sorting enriches osteogenic populations in rat bone marrow stromal cell cultures" @default.
• W2171067064 cites W1211597803 @default.
• W2171067064 cites W1494887369 @default.
• W2171067064 cites W1555330200 @default.
• W2171067064 cites W1574963476 @default.
• W2171067064 cites W1586829831 @default.
• W2171067064 cites W1872911786 @default.
• W2171067064 cites W1965174761 @default.
• W2171067064 cites W1972830850 @default.
• W2171067064 cites W1976538215 @default.
• W2171067064 cites W1980187813 @default.
• W2171067064 cites W1982408947 @default.
• W2171067064 cites W1982486177 @default.
• W2171067064 cites W198503490 @default.
• W2171067064 cites W1986276800 @default.
• W2171067064 cites W1990854759 @default.
• W2171067064 cites W1993214982 @default.
• W2171067064 cites W1995613052 @default.
• W2171067064 cites W2001783191 @default.
• W2171067064 cites W2005057936 @default.
• W2171067064 cites W2008021821 @default.
• W2171067064 cites W2008941474 @default.
• W2171067064 cites W2010825711 @default.
• W2171067064 cites W2019480285 @default.
• W2171067064 cites W2020616952 @default.
• W2171067064 cites W2022324982 @default.
• W2171067064 cites W2037152054 @default.
• W2171067064 cites W2043308964 @default.
• W2171067064 cites W2043651158 @default.
• W2171067064 cites W2048130804 @default.
• W2171067064 cites W2055690022 @default.
• W2171067064 cites W2062504097 @default.
• W2171067064 cites W2067112519 @default.
• W2171067064 cites W2074681052 @default.
• W2171067064 cites W2080473972 @default.
• W2171067064 cites W2087030052 @default.
• W2171067064 cites W2091423691 @default.
• W2171067064 cites W2091799660 @default.
• W2171067064 cites W2093927588 @default.
• W2171067064 cites W2097053416 @default.
• W2171067064 cites W2100931541 @default.
• W2171067064 cites W2101146104 @default.
• W2171067064 cites W2121390857 @default.
• W2171067064 cites W2122888633 @default.
• W2171067064 cites W2127684770 @default.
• W2171067064 cites W2129812644 @default.
• W2171067064 cites W2131598671 @default.
• W2171067064 cites W2146288764 @default.
• W2171067064 cites W2166460029 @default.
• W2171067064 cites W2170178982 @default.
• W2171067064 cites W2378595245 @default.
• W2171067064 cites W2467132418 @default.
• W2171067064 cites W4232470425 @default.
• W2171067064 cites W4251504254 @default.
• W2171067064 cites W4292330637 @default.
• W2171067064 cites W84497161 @default.
• W2171067064 doi "https://doi.org/10.1016/s8756-3282(97)00197-x" @default.
• W2171067064 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9430238" @default.
• W2171067064 hasPublicationYear "1997" @default.
• W2171067064 type Work @default.
• W2171067064 sameAs 2171067064 @default.
• W2171067064 citedByCount "49" @default.
• W2171067064 countsByYear W21710670642012 @default.
• W2171067064 countsByYear W21710670642013 @default.
• W2171067064 countsByYear W21710670642014 @default.
• W2171067064 countsByYear W21710670642015 @default.
• W2171067064 countsByYear W21710670642016 @default.
• W2171067064 countsByYear W21710670642017 @default.
• W2171067064 countsByYear W21710670642019 @default.
• W2171067064 countsByYear W21710670642020 @default.
• W2171067064 crossrefType "journal-article" @default.
• W2171067064 hasAuthorship W2171067064A5031900884 @default.
• W2171067064 hasAuthorship W2171067064A5056773555 @default.
• W2171067064 hasConcept C126322002 @default.
• W2171067064 hasConcept C153911025 @default.
• W2171067064 hasConcept C16930146 @default.
• W2171067064 hasConcept C185592680 @default.
• W2171067064 hasConcept C202751555 @default.
• W2171067064 hasConcept C203014093 @default.
• W2171067064 hasConcept C2778260815 @default.
• W2171067064 hasConcept C2778630268 @default.
• W2171067064 hasConcept C2780007613 @default.
• W2171067064 hasConcept C2908647359 @default.
• W2171067064 hasConcept C502942594 @default.
• W2171067064 hasConcept C553184892 @default.
• W2171067064 hasConcept C55493867 @default.
• W2171067064 hasConcept C71924100 @default.
• W2171067064 hasConcept C86803240 @default.
• W2171067064 hasConcept C95444343 @default.
• W2171067064 hasConcept C99454951 @default.
• W2171067064 hasConceptScore W2171067064C126322002 @default.
• W2171067064 hasConceptScore W2171067064C153911025 @default.

• next