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• W2171216076 abstract "Prunus necrotic ring spot virus (PNRSV) was isolate d for the first time in Egypt from naturally infected rose plants collected from the e xperimental farm of the Faculty of Agriculture, Cairo University). Observed symptoms circumvented nring spots on leaves, bud failure, and color breaking of petals. The virus was transmitted mechanically. The purified virus had Amax and A min at 260 and 240 nm respectively. The 260/280 ratio was 1.56. Yield of purified virus from infected Gompherina globosa was 0.182 mg/g tissue. Electron micrograph of the purified virus showed spherical (23-nm) as well as bacilliform vir us particles (42x23 nm). The induced antiserum from the purified virus was successfully used to de tect PNRSV in rose plants in several locations in Egypt. The full length of the replicase gene of PNR SV was successfully amplified by reverse transcription-polymerase chain reaction (RT-PCR) us ing different sets of specific primers. A sensitive and specific IC-RT-PCR protocol was used for the detection of PNRSV from rose tissues. Sequence analysis of PNRSV/rep gene of the rose iso late indicated 60 % similarity to that of PNRSV-AF278534 and NC-004362." @default.
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• W2171216076 date "2008-01-01" @default.
• W2171216076 modified "2023-09-27" @default.
• W2171216076 title "Biological, serological and molecular studies on Prunus necrotic ring spot virus infecting Rosa hybrida L. in Egypt" @default.
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