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• W2171325607 abstract "Abstract Background The turbot ( Scophthalmus maximus ; Scophthalmidae; Pleuronectiformes) is a flatfish species of great relevance for marine aquaculture in Europe. In contrast to other cultured flatfish, very few genomic resources are available in this species. Aeromonas salmonicida and Philasterides dicentrarchi are two pathogens that affect turbot culture causing serious economic losses to the turbot industry. Little is known about the molecular mechanisms for disease resistance and host-pathogen interactions in this species. In this work, thousands of ESTs for functional genomic studies and potential markers linked to ESTs for mapping (microsatellites and single nucleotide polymorphisms (SNPs)) are provided. This information enabled us to obtain a preliminary view of regulated genes in response to these pathogens and it constitutes the basis for subsequent and more accurate microarray analysis. Results A total of 12584 cDNAs partially sequenced from three different cDNA libraries of turbot ( Scophthalmus maximus ) infected with Aeromonas salmonicida , Philasterides dicentrarchi and from healthy fish were analyzed. Three immune-relevant tissues (liver, spleen and head kidney) were sampled at several time points in the infection process for library construction. The sequences were processed into 9256 high-quality sequences, which constituted the source for the turbot EST database. Clustering and assembly of these sequences, revealed 3482 different putative transcripts, 1073 contigs and 2409 singletons. BLAST searches with public databases detected significant similarity (e-value ≤ 1e-5) in 1766 (50.7%) sequences and 816 of them (23.4%) could be functionally annotated. Two hundred three of these genes (24.9%), encoding for defence/immune-related proteins, were mostly identified for the first time in turbot. Some ESTs showed significant differences in the number of transcripts when comparing the three libraries, suggesting regulation in response to these pathogens. A total of 191 microsatellites, with 104 having sufficient flanking sequences for primer design, and 1158 putative SNPs were identified from these EST resources in turbot. Conclusion A collection of 9256 high-quality ESTs was generated representing 3482 unique turbot sequences. A large proportion of defence/immune-related genes were identified, many of them regulated in response to specific pathogens. Putative microsatellites and SNPs were identified. These genome resources constitute the basis to develop a microarray for functional genomics studies and marker validation for genetic linkage and QTL analysis in turbot." @default.
• W2171325607 created "2016-06-24" @default.
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• W2171325607 date "2008-09-25" @default.
• W2171325607 modified "2023-10-09" @default.
• W2171325607 title "Expressed sequence tags (ESTs) from immune tissues of turbot (Scophthalmus maximus) challenged with pathogens" @default.
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• W2171325607 cites W1974829152 @default.
• W2171325607 cites W1980208188 @default.
• W2171325607 cites W1982437222 @default.
• W2171325607 cites W1986825290 @default.
• W2171325607 cites W1988713956 @default.
• W2171325607 cites W1988832109 @default.
• W2171325607 cites W1995933541 @default.
• W2171325607 cites W1999790144 @default.
• W2171325607 cites W2005541898 @default.
• W2171325607 cites W2008169330 @default.
• W2171325607 cites W2010741070 @default.
• W2171325607 cites W2018300574 @default.
• W2171325607 cites W2019354318 @default.
• W2171325607 cites W2035755870 @default.
• W2171325607 cites W2038835012 @default.
• W2171325607 cites W2048553024 @default.
• W2171325607 cites W2048775827 @default.
• W2171325607 cites W2052229577 @default.
• W2171325607 cites W2054557284 @default.
• W2171325607 cites W2055043387 @default.
• W2171325607 cites W2058304642 @default.
• W2171325607 cites W2064912476 @default.
• W2171325607 cites W2065267686 @default.
• W2171325607 cites W2065618297 @default.
• W2171325607 cites W2066110492 @default.
• W2171325607 cites W2067515946 @default.
• W2171325607 cites W2072649421 @default.
• W2171325607 cites W2080042397 @default.
• W2171325607 cites W2081825977 @default.
• W2171325607 cites W2087770569 @default.
• W2171325607 cites W2091366683 @default.
• W2171325607 cites W2092225512 @default.
• W2171325607 cites W2094448442 @default.
• W2171325607 cites W2096888246 @default.
• W2171325607 cites W2102063582 @default.
• W2171325607 cites W2103017472 @default.
• W2171325607 cites W2107925769 @default.
• W2171325607 cites W2119319311 @default.
• W2171325607 cites W2119501580 @default.
• W2171325607 cites W2119923823 @default.
• W2171325607 cites W2121016876 @default.
• W2171325607 cites W2125439994 @default.
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• W2171325607 doi "https://doi.org/10.1186/1746-6148-4-37" @default.
• W2171325607 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2569028" @default.
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