FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171382425> ?p ?o ?g. }

• W2171382425 endingPage "507" @default.
• W2171382425 startingPage "495" @default.
• W2171382425 abstract "A rat epididymal protein of 27 kDa was identified using neonatal tolerization. This study reports the production and characterization of a polyclonal antiserum to this protein. ELISA was used to demonstrate that this antiserum reacts strongly with epididymal sperm proteins, but has little or no reactivity with testicular proteins. Western blot analysis revealed that this polyclonal antiserum recognized a 27 kDa protein extracted from the corpus epididymidis as well as from spermatozoa from the corpus and cauda epididymides, and immunostaining revealed the presence of the protein in the corpus to cauda epididymides. Stronger reactivity was observed in the supranuclear region and stereocilla of principal cells of the corpus epididymidis and in the luminal content of the corpus and cauda epididymides. The testicular section showed no reactivity. Treatment with the antiserum resulted in time- and dose-dependent agglutination of rat spermatozoa. By indirect immunofluorescence, the antiserum localized proteins in the mid-piece region of rat spermatozoa. Studies were carried out to determine the age at which the protein first became apparent during postnatal development. The protein was expressed from day 40 onwards, as demonstrated by western blot analysis. The androgen regulation of this protein was ascertained by castration and supplementation studies. Expression of this protein showed a decline starting at day 14 after castration and by day 21 the protein was absent; however, androgen replacement resulted in the reappearance of the protein. The results of these studies indicate that the protein identified is specific to the epididymis, and is regulated by development and androgens. The importance of epididymis-specific proteins that are regulated by androgens in sperm maturation is discussed, and the need to ascertain the sequence of the protein and clone the cognate gene is indicated." @default.
• W2171382425 created "2016-06-24" @default.
• W2171382425 creator A5007321704 @default.
• W2171382425 creator A5014155001 @default.
• W2171382425 creator A5045351679 @default.
• W2171382425 creator A5089773630 @default.
• W2171382425 date "2003-04-01" @default.
• W2171382425 modified "2023-10-18" @default.
• W2171382425 title "Postnatal development and testosterone dependence of a rat epididymal protein identified by neonatal tolerization" @default.
• W2171382425 cites W1596682143 @default.
• W2171382425 cites W162689908 @default.
• W2171382425 cites W1775749144 @default.
• W2171382425 cites W1973938025 @default.
• W2171382425 cites W1981559854 @default.
• W2171382425 cites W2004907268 @default.
• W2171382425 cites W2005336167 @default.
• W2171382425 cites W2012184460 @default.
• W2171382425 cites W2014915865 @default.
• W2171382425 cites W2026489420 @default.
• W2171382425 cites W2044456805 @default.
• W2171382425 cites W2045026009 @default.
• W2171382425 cites W2046557362 @default.
• W2171382425 cites W2046659306 @default.
• W2171382425 cites W2055563544 @default.
• W2171382425 cites W2073735914 @default.
• W2171382425 cites W2081607413 @default.
• W2171382425 cites W2089518190 @default.
• W2171382425 cites W2101108802 @default.
• W2171382425 cites W2114045571 @default.
• W2171382425 cites W2127120875 @default.
• W2171382425 cites W2143944292 @default.
• W2171382425 cites W2148824721 @default.
• W2171382425 cites W2148931507 @default.
• W2171382425 cites W2149144525 @default.
• W2171382425 cites W2163634502 @default.
• W2171382425 cites W2270311910 @default.
• W2171382425 cites W2302033019 @default.
• W2171382425 cites W2410169143 @default.
• W2171382425 cites W2411215854 @default.
• W2171382425 cites W2412061998 @default.
• W2171382425 cites W2413491149 @default.
• W2171382425 cites W2417741869 @default.
• W2171382425 cites W2418371621 @default.
• W2171382425 cites W94651834 @default.
• W2171382425 doi "https://doi.org/10.1530/rep.0.1250495" @default.
• W2171382425 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12683920" @default.
• W2171382425 hasPublicationYear "2003" @default.
• W2171382425 type Work @default.
• W2171382425 sameAs 2171382425 @default.
• W2171382425 citedByCount "12" @default.
• W2171382425 countsByYear W21713824252012 @default.
• W2171382425 countsByYear W21713824252015 @default.
• W2171382425 countsByYear W21713824252022 @default.
• W2171382425 countsByYear W21713824252023 @default.
• W2171382425 crossrefType "journal-article" @default.
• W2171382425 hasAuthorship W2171382425A5007321704 @default.
• W2171382425 hasAuthorship W2171382425A5014155001 @default.
• W2171382425 hasAuthorship W2171382425A5045351679 @default.
• W2171382425 hasAuthorship W2171382425A5089773630 @default.
• W2171382425 hasBestOaLocation W21713824251 @default.
• W2171382425 hasConcept C104317684 @default.
• W2171382425 hasConcept C126322002 @default.
• W2171382425 hasConcept C134018914 @default.
• W2171382425 hasConcept C153911025 @default.
• W2171382425 hasConcept C159654299 @default.
• W2171382425 hasConcept C16685009 @default.
• W2171382425 hasConcept C203014093 @default.
• W2171382425 hasConcept C204232928 @default.
• W2171382425 hasConcept C2776415932 @default.
• W2171382425 hasConcept C2778236600 @default.
• W2171382425 hasConcept C2779256933 @default.
• W2171382425 hasConcept C2779279991 @default.
• W2171382425 hasConcept C2781087480 @default.
• W2171382425 hasConcept C2781254305 @default.
• W2171382425 hasConcept C35866371 @default.
• W2171382425 hasConcept C4224716 @default.
• W2171382425 hasConcept C55493867 @default.
• W2171382425 hasConcept C71315377 @default.
• W2171382425 hasConcept C71924100 @default.
• W2171382425 hasConcept C85265743 @default.
• W2171382425 hasConcept C86803240 @default.
• W2171382425 hasConcept C91523082 @default.
• W2171382425 hasConcept C956191 @default.
• W2171382425 hasConceptScore W2171382425C104317684 @default.
• W2171382425 hasConceptScore W2171382425C126322002 @default.
• W2171382425 hasConceptScore W2171382425C134018914 @default.
• W2171382425 hasConceptScore W2171382425C153911025 @default.
• W2171382425 hasConceptScore W2171382425C159654299 @default.
• W2171382425 hasConceptScore W2171382425C16685009 @default.
• W2171382425 hasConceptScore W2171382425C203014093 @default.
• W2171382425 hasConceptScore W2171382425C204232928 @default.
• W2171382425 hasConceptScore W2171382425C2776415932 @default.
• W2171382425 hasConceptScore W2171382425C2778236600 @default.
• W2171382425 hasConceptScore W2171382425C2779256933 @default.
• W2171382425 hasConceptScore W2171382425C2779279991 @default.
• W2171382425 hasConceptScore W2171382425C2781087480 @default.
• W2171382425 hasConceptScore W2171382425C2781254305 @default.
• W2171382425 hasConceptScore W2171382425C35866371 @default.

• next