FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2171431330> ?p ?o ?g. }

• W2171431330 endingPage "13" @default.
• W2171431330 startingPage "13" @default.
• W2171431330 abstract "Retinal phosducin (Phd) and phosducin-like protein 1 (PhLP1) selectively bind G-protein beta/gamma subunits (Gbetagamma). Our laboratory has recently identified two phosducin-like orphan proteins (PhLOP1 and PhLOP2) that lack the ability to interact with Gbetagamma. In search of potential functional protein partner(s) for these phosducin orphans, we examined their protein-protein interactions using a yeast two-hybrid screen.A bovine retina yeast expression cDNA library was screened with the GAL4 DNA binding domain (BD) fusion of PhLOP1. Quantitative analysis of the selected positives with PhLOP1 and other Phd isoforms was assessed by growth and beta-galactosidase activity. Further molecular, biochemical, and immunological detection methods utilizing glutathione S-transferase (GST)-Phd isoform fusion proteins and the potential partner were also performed.A member of the superfamily of putative ATPases was selected in the yeast two hybrid screen. Further characterization identified a direct interaction of this putative ATPase with PhLOP1, as well as Phd and PhLP1, but not with PhLOP2. A database search verified this ATPase as a bovine orthologue of the yeast SUG1 (ySUG1), a putative transcriptional mediator and a subunit of the 26S proteasome complex. Our experiments reveal that the carboxy-terminus of PhLOP1 is essential for the protein-protein interaction with SUG1, but it alone is not sufficient to mediate SUG1 interaction.Based on these experimental results, Phd, PhLP1 and PhLOP1 have protein-protein interaction with SUG1. PhLOP1, a truncated amino-terminal splice variant of Phd, is the best candidate for the interaction with SUG1 among the four Phd isoforms studied, which suggests a potential function for PhLOP1." @default.
• W2171431330 created "2016-06-24" @default.
• W2171431330 creator A5027422232 @default.
• W2171431330 creator A5071772212 @default.
• W2171431330 date "1998-08-11" @default.
• W2171431330 modified "2023-09-27" @default.
• W2171431330 title "Interaction of phosducin and phosducin isoforms with a 26S proteasomal subunit, SUG1." @default.
• W2171431330 cites W1494429877 @default.
• W2171431330 cites W1508171779 @default.
• W2171431330 cites W1554470867 @default.
• W2171431330 cites W1562107474 @default.
• W2171431330 cites W1570444949 @default.
• W2171431330 cites W1918899468 @default.
• W2171431330 cites W1964634901 @default.
• W2171431330 cites W1964931840 @default.
• W2171431330 cites W1968707041 @default.
• W2171431330 cites W1971626991 @default.
• W2171431330 cites W1981513994 @default.
• W2171431330 cites W1992518796 @default.
• W2171431330 cites W1999255494 @default.
• W2171431330 cites W2001768785 @default.
• W2171431330 cites W2006393374 @default.
• W2171431330 cites W2009433942 @default.
• W2171431330 cites W2011796598 @default.
• W2171431330 cites W2015318317 @default.
• W2171431330 cites W2028876723 @default.
• W2171431330 cites W2029475936 @default.
• W2171431330 cites W2030803127 @default.
• W2171431330 cites W2038580923 @default.
• W2171431330 cites W2042258280 @default.
• W2171431330 cites W2043937551 @default.
• W2171431330 cites W2047369216 @default.
• W2171431330 cites W2049471507 @default.
• W2171431330 cites W2049679687 @default.
• W2171431330 cites W2050145162 @default.
• W2171431330 cites W2065440455 @default.
• W2171431330 cites W2072163638 @default.
• W2171431330 cites W2074662488 @default.
• W2171431330 cites W2077848026 @default.
• W2171431330 cites W2078528548 @default.
• W2171431330 cites W2079696832 @default.
• W2171431330 cites W2080382431 @default.
• W2171431330 cites W2080892506 @default.
• W2171431330 cites W2082290760 @default.
• W2171431330 cites W2087148381 @default.
• W2171431330 cites W2090406117 @default.
• W2171431330 cites W2094168150 @default.
• W2171431330 cites W2138408156 @default.
• W2171431330 cites W2142946131 @default.
• W2171431330 cites W2149920358 @default.
• W2171431330 cites W2411914739 @default.
• W2171431330 cites W2413424377 @default.
• W2171431330 cites W2601067341 @default.
• W2171431330 cites W2613616550 @default.
• W2171431330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9701609" @default.
• W2171431330 hasPublicationYear "1998" @default.
• W2171431330 type Work @default.
• W2171431330 sameAs 2171431330 @default.
• W2171431330 citedByCount "6" @default.
• W2171431330 countsByYear W21714313302017 @default.
• W2171431330 crossrefType "journal-article" @default.
• W2171431330 hasAuthorship W2171431330A5027422232 @default.
• W2171431330 hasAuthorship W2171431330A5071772212 @default.
• W2171431330 hasConcept C104292427 @default.
• W2171431330 hasConcept C104317684 @default.
• W2171431330 hasConcept C11804247 @default.
• W2171431330 hasConcept C123894998 @default.
• W2171431330 hasConcept C153911025 @default.
• W2171431330 hasConcept C174851938 @default.
• W2171431330 hasConcept C187882448 @default.
• W2171431330 hasConcept C189482833 @default.
• W2171431330 hasConcept C2779222958 @default.
• W2171431330 hasConcept C40767141 @default.
• W2171431330 hasConcept C53345823 @default.
• W2171431330 hasConcept C55493867 @default.
• W2171431330 hasConcept C86803240 @default.
• W2171431330 hasConcept C95444343 @default.
• W2171431330 hasConceptScore W2171431330C104292427 @default.
• W2171431330 hasConceptScore W2171431330C104317684 @default.
• W2171431330 hasConceptScore W2171431330C11804247 @default.
• W2171431330 hasConceptScore W2171431330C123894998 @default.
• W2171431330 hasConceptScore W2171431330C153911025 @default.
• W2171431330 hasConceptScore W2171431330C174851938 @default.
• W2171431330 hasConceptScore W2171431330C187882448 @default.
• W2171431330 hasConceptScore W2171431330C189482833 @default.
• W2171431330 hasConceptScore W2171431330C2779222958 @default.
• W2171431330 hasConceptScore W2171431330C40767141 @default.
• W2171431330 hasConceptScore W2171431330C53345823 @default.
• W2171431330 hasConceptScore W2171431330C55493867 @default.
• W2171431330 hasConceptScore W2171431330C86803240 @default.
• W2171431330 hasConceptScore W2171431330C95444343 @default.
• W2171431330 hasLocation W21714313301 @default.
• W2171431330 hasOpenAccess W2171431330 @default.
• W2171431330 hasPrimaryLocation W21714313301 @default.
• W2171431330 hasRelatedWork W1978299773 @default.
• W2171431330 hasRelatedWork W1983834588 @default.
• W2171431330 hasRelatedWork W2026524456 @default.
• W2171431330 hasRelatedWork W2062675956 @default.

• next