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- W2171477438 abstract "To investigate the mechanism of spliceosome assembly in vivo, we performed chromatin immunoprecipitation (ChIP) analysis of U1, U2, and U5 small nuclear ribonucleoprotein particles (snRNPs) to intron-containing yeast (S. cerevisiae) genes. The snRNPs display patterns that indicate a cotranscriptional assembly model: U1 first, then U2, and the U4/U6•U5 tri-snRNP followed by U1 destabilization. cis-splicing mutations also support a role of U2 and/or the tri-snRNP in U1 destabilization. Moreover, they indicate that splicing efficiency has a major impact on cotranscriptional snRNP recruitment and suggest that cotranscriptional recruitment of U2 or the tri-snRNP is required to commit the pre-mRNA to splicing. Branchpoint (BP) mutations had a major effect on the U1 pattern, whereas 5′ splice site (5′ss) mutations had a stronger effect on the U2 pattern. A 5′ss-U1 snRNA complementation experiment suggests that pairing between U1 and the 5′ss occurs after U1 recruitment and contributes to a specific U1:substrate conformation required for efficient U2 and tri-snRNP recruitment." @default.
- W2171477438 created "2016-06-24" @default.
- W2171477438 creator A5066684109 @default.
- W2171477438 creator A5068402917 @default.
- W2171477438 date "2005-07-01" @default.
- W2171477438 modified "2023-10-10" @default.
- W2171477438 title "Cotranscriptional Spliceosome Assembly Dynamics and the Role of U1 snRNA:5′ss Base Pairing in Yeast" @default.
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- W2171477438 doi "https://doi.org/10.1016/j.molcel.2005.05.006" @default.
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